Relative gene expression of fatty acid synthesis genes at 60 days postpartum in bovine mammary epithelial cells of Surti and Jafarabadi buffaloes

Abstract

Aim:Aim of the study was to study the relative gene expression of genes associated with fatty acid synthesis at 60 days postpartum (pp) in bovine mammary epithelial cells (MECs) of Surti and Jafarabadi buffaloes.Materials and Methods:A total of 10 healthy Surti and Jafarabadi buffaloes of each breed were selected at random from Livestock Research Station, Navsari and Cattle Breeding Farm, Junagadh, Gujarat, respectively, for this study. Milk sample was collected from each selected buffalo at day 60 pp from these two breeds to study relative gene expression of major milk fat genes using non-invasive approach of obtaining primary bovine MECs (pBMEC) from milk samples.Results:In this study overall, the relative expression of the six major milk lipogenic genes butyrophilin subfamily 1 member A1 (BTN1A1), stearoyl-CoA desaturase (SCD), lipoprotein lipase (LPL), glycerol-3-phosphate acyltransferase mitochondrial (GPAM), acetyl-coenzyme A carboxylase alpha (ACACA), and lipin (LPIN) did not show changes in expression patterns at 60th day of lactation in both Surti and Jafarabadi buffaloes.Conclusion:The pBMEC can be successfully recovered from 1500 ml of milk of Surti and Jafarabadi buffaloes using antibody-mediated magnetic bead separation and can be further used for recovering RNA for down step quantification of major milk lipogenic gene expression. The relative expression of the six major milk lipogenic genes BTN1A1, SCD, LPL, GPAM, ACACA, and LPIN did not show changes in expression patterns in both Surti and Jafarabadi buffaloes, suggesting expression levels of lipogenic genes are maintained almost uniform till peak lactation without any significant difference.

Mammary fat globules as a source of mRNA to model alterations in the expression of some milk component genes during lactation in bovines

“…According to our data, LPL gene expression is highest in Egyptian Buffalo compared to the two HF breeds specially at early lactation stage compared to peak stage. These findings were consistent with those of M Janmeda, V Kharadi, G Pandya, B Brahmkshtri, U Ramani and K Tyagi [ 43 ], who found that, in Surti buffalo, LPL expression was highest on 15th day after calving, then decreased till day 60. Non-esterified free Fatty acids (NEFA) and lipoproteins supply long chain fatty acids (LCFA) to mammary cells, this may explain why the LPL gene is expressed at a higher level during early lactation.…”

“…As well as, E Sandri, K Harvatine and D Oliveira [ 20 ] found that LPL gene expression in Holstein cattle was highest during the peak [DIM = 60 days] stage of lactation and subsequently dropped during the late stage of lactation. Jaffarabadi buffalo, on the other hand, showed no variation in LPL expression between days 15 and 60 of lactation (M Janmeda, V Kharadi, G Pandya, B Brahmkshtri, U Ramani and K Tyagi [ 43 ]. Similarities between the lactation curve and LPL expression suggest a potential role for this gene in sustaining milk synthesis.…”