Relative Host Resistance to Black Spot Disease in Field Pea (<i>Pisum sativum</i>) is Determined by Individual Pathogens

Tran, Hieu Sy; You, Ming Pei; Khan, Tanveer; Barbetti, Martin J.

doi:10.1094/pdis-06-14-0655-re

Cited by 15 publications

(27 citation statements)

References 45 publications

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“…exigua (Li et al ., ) and Phoma glomerata (Tran et al ., ). Of these latter pathogens, Phoma koolunga has recently been shown to be widespread across Australia in association with field pea black spot (Davidson et al ., ; Tran et al ., ) and also to be a highly virulent pathogen to field pea (Tran et al ., ).…”

Section: Introductionmentioning

confidence: 81%

“…Comparison conidial germination assays in sterile distilled water on glass slides were also implemented. Because resistances against P. koolunga are different on leaves and stem (Tran et al ., ), different susceptible and resistant genotypes were used for observation on each kind of tissue. The pea genotypes used for study of the infection processes were chosen from a screening of 40 pea genotypes for resistance of leaf and stem to D. pinodes , P. pinodella and P. koolunga in 2014 conducted by Tran et al .…”

Section: Methodsmentioning

confidence: 97%

“…Tran et al . () reported that among the three main pathogens of the black spot complex in Western Australia tested on field pea stems, i.e. D. pinodes , P. pinodella and P. koolunga , the latter was the most virulent.…”

Section: Introductionmentioning

confidence: 97%

“…In addition, P. koolunga caused significantly higher levels of disease on leaves than D. pinodes (Tran et al . (), challenging the long held opinion that D. pinodes is universally the most important pathogen in the black spot complex (Bretag et al ., ; Tivoli & Banniza, ). Further, P. koolunga causes disease to the below ground parts of field pea, similar to D. pinodes and P. pinodella , with Tran et al .…”

Section: Introductionmentioning

confidence: 99%

“…While there have been no studies of the infection process for P. koolunga , recent studies by Tran et al . (), highlighting differential resistant and susceptible host leaf and stem responses to this pathogen, have opened the way for studies into the infection process for this pathogen on field pea. Therefore, this is the first study to investigate the infection process of P. koolunga on leaf and stem of both resistant and susceptible pea genotypes.…”

Section: Introductionmentioning

confidence: 99%

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Infection process of Phoma koolunga on stem and leaf tissue of resistant and susceptible field pea (Pisum sativum)

et al. 2016

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This study investigates the infection process of Phoma koolunga on field pea (Pisum sativum) stems and leaves using different susceptible and resistant pea genotypes for each tissue, viz. 05P778‐BSR‐701 (resistant) and 06P830‐(F5)‐BSR‐5 (susceptible) for stems and ATC 866 (resistant) and ATC 5347 (susceptible) for leaves. On both resistant and susceptible genotypes, light and scanning electron microscopy showed P. koolunga conidia infect stem and leaf tissues directly via appressoria or stomatal penetration, but with more infections involving formation of appressoria on stems than on leaves. On leaves of the resistant genotype, at 72 h post‐inoculation, P. koolunga penetrated more frequently via stomata (5.2 conidia per 36 893 μm2) than by formation of appressoria (1.8 conidia); yet no such difference was observed on stems of the resistant genotype. In contrast, at the same time point, the number of conidia infecting the susceptible genotype by formation of appressoria on either stems (135 conidia) or leaves (11.3 conidia) was significantly greater than via stomata (8 and 7.3 conidia, stems and leaves, respectively). Mean germ tube length of germinating P. koolunga conidia on both stems (29.8 μm) and leaves (32.9 μm) of the resistant genotype was less than on the susceptible genotype (40.5 and 63.7 μm, stem and leaves, respectively). In addition, there were differences in the number of germ tubes emerging from conidia on resistant and susceptible genotypes. These are the first insights into the nature of leaf and stem resistance mechanisms operating in field pea against P. koolunga.

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