Relative importance of seed‐tuber and soilborne inoculum in causing black dot disease of potato

Lees, A. K.; Brierley, J. L.; Stewart, Jennifer A.; Hilton, Anne; Wale, S. J.; Gladders, P.; Bradshaw, N. J.; Peters, J. C.

doi:10.1111/j.1365-3059.2010.02284.x

Cited by 19 publications

(29 citation statements)

References 19 publications

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“…Lees et al . () reported that in controlled environment experiments black dot severity was higher in the damp treatment compared to the treatment with restricted water (half that given to damp treatments). Olanya et al .…”

Section: Discussionmentioning

confidence: 95%

Factors affecting the development and control of black dot on potato tubers

et al. 2014

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Field trials were carried out over a 4 year period (2004)(2005)(2006)(2007) to determine the effect of agronomic factors, specifically cultivar resistance, irrigation, crop duration and chemical control (in-furrow application of azoxystrobin), on black dot development on potato tubers grown in fields where soilborne inoculum of Colletotrichum coccodes was present. In 2004In , 2005In and 2006, two field trials were performed each year and in 2007, 19 mini-field trials were carried out across Scotland and England. Cultivar resistance was clearly demonstrated to be an effective method of reducing black dot disease severity on tubers (described here as the percentage of unmarketable tubers, i.e. those with symptoms covering a surface area of >10%). In the four field trials carried out in 2004 and 2005, in irrigated and fungicide-untreated plots, 43Á8% of tubers of cv. Maris Piper were unmarketable, compared with 17Á0% of tubers of cv. Sante. Assessments of disease development on underground plant parts (stems, stolons and roots) revealed that cultivar resistance acted only at the tuber level, as disease symptoms on other parts were often high irrespective of published disease resistance ratings. Irrigation increased the severity of disease on tubers in two trials (England 2004 and 2006), but its effect was less significant when rainfall was high. Delaying harvest by 2 weeks increased disease severity in all six trials, whilst application of azoxystrobin consistently reduced black dot severity. There were significant interactions between factors. The results clearly show how black dot disease severity can be reduced through an integrated approach to disease management.

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Section: Discussionmentioning

confidence: 95%

Factors affecting the development and control of black dot on potato tubers

et al. 2014

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“…Twenty‐seven soils sampled from potato fields in 2006 (as detailed by Lees et al. , 2010) were tested using the soil DNA extraction method of Brierley et al.…”

Section: Methodsmentioning

confidence: 99%

Development of a quantitative real‐time PCR assay for Phytophthora infestans and its applicability to leaf, tuber and soil samples

et al. 2012

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A sensitive real-time polymerase chain reaction (PCR) assay was developed for the quantification of Phytophthora infestans, the cause of foliar and tuber late blight in potato. A primer pair (PinfTQF ⁄ PinfTQR) and a fluorogenic probe (PinfTQPR) were designed to perform a quantitative assay for the detection of P. infestans in leaves, tubers and soils. The assay was shown to be specific to P. infestans and the very closely taxonomically related non-potato pathogen species P. mirabilis, P. phaseoli and P. ipomoea, but did not detect the potato pathogens P. erythroseptica and P. nicotianae. The assay was able to reliably detect P. infestans DNA at 100 fg per reaction and was effective in quantifying P. infestans in infected leaf tissue from 24 h after inoculation and also in infected symptomless tubers and diseased tubers. Attempts to detect oospores of P. infestans in naturally and artificially infested soil samples are described and compared with baiting tests and previous literature. It was not possible to detect oospores in soil samples due to problems with DNA extraction from the oospores themselves. However, the assay was shown to detect even very low levels of asexual inoculum (sporangia and mycelium) in soil. This work assembles all the necessary features of a quantitative P. infestans assay, which have previously been somewhat disparate: the sensitivity, specificity and quantitation are fully validated, the assay is shown to work in common applications in leaf and tuber tissue and the problems with P. infestans oospore detection are explored and tested experimentally.

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“…Therefore the denser sampling strategy is most cost-effective with regard to the potential for a more reliable prediction. The sampling density of 25 cores/sampling points per ha is similar to sampling densities used for detection and quantification of other soil-borne pathogens, like Colletotrichum coccodes and Rhizoctonia solani in potatoes in the UK (Lees et al 2010;Peters et al 2011).…”

Section: Discussionmentioning

confidence: 98%

Detection and prediction of post harvest carrot diseases

et al. 2011

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Specific PCR primers were developed for identifying two post harvest pathogens, Mycocentrospora acerina and Fibularhizoctonia carotae, which cause liquorice rot and crater rot respectively, during prolonged low temperature storage of carrots. The methods allow routine detection of less than 0.3 pg of M. acerina DNA and less than 0.03 pg F. carotae DNA, even in the presence of large excess of plant or soil DNA. Standard PCR and quantitative PCR gave similar results and either method could be used in a practical situation. Experiments were carried out testing these methods on different types of carrot tissue-and soil-samples. Soil was sampled before sowing, and soil adhering to the roots or root tissue was sampled at different times during the growing season or at harvest. Soil adhering to the carrots at harvest had the best predictive ability for liquorice rot development during storage (R 2 predicted 74.9% using standard PCR), but samples taken during the growing season also gave reasonably good predictive ability values. PCR data from soil samples taken in the spring were not as good as a predictor for this disease. A dense sampling strategy using 20 m between sampling points generally gave better correlation between PCR data and disease data than using 40 m between the sampling points. Use of the developed methods in an IPM strategy for liquorice rot is discussed. For crater rot the correlation between PCR data and disease data was generally poor for all types of samples. These results are discussed in relation to the biology of F. carotae.

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Relative importance of seed‐tuber and soilborne inoculum in causing black dot disease of potato

Cited by 19 publications

References 19 publications

Factors affecting the development and control of black dot on potato tubers

Factors affecting the development and control of black dot on potato tubers

Development of a quantitative real‐time PCR assay for Phytophthora infestans and its applicability to leaf, tuber and soil samples

Detection and prediction of post harvest carrot diseases

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