Relative Incidence of
            <i>Alteromonas putrefaciens</i>
            and
            <i>Pseudomonas putrefaciens</i>
            in Ground Beef

Parker, Louise L.; Levin, Robert E.

doi:10.1128/aem.45.3.796-799.1983

Cited by 14 publications

(3 citation statements)

References 24 publications

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“…Amino acids or mixtures of peptides have been employed as electron donors for S. putrefaciens growing by iron reduction (29). S. putrefaciens has long been associated with fish pathologies and the spoilage of proteinaceous foods (14,41,51). Thus, amino acids may play an unappreciated role in the anaerobic metabolism of these bacteria.…”

Section: Discussionmentioning

confidence: 99%

Growth of the facultative anaerobe Shewanella putrefaciens by elemental sulfur reduction

Moser

Nealson

1996

Appl Environ Microbiol

123

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The growth of bacteria by dissimilatory elemental sulfur reduction is generally associated with obligate anaerobes and thermophiles in particular. Here we describe the sulfur-dependent growth of the facultatively anaerobic mesophile Shewanella putrefaciens. Six of nine representative S. putrefaciens isolates from a variety of environments proved able to grow by sulfur reduction, and strain MR-1 was chosen for further study. Growth was monitored in a minimal medium (usually with 0.05% Casamino Acids added as a growth stimulant) containing 30 mM lactate and limiting concentrations of elemental sulfur. When mechanisms were provided for the removal of the metabolic end product, H2S, measurable growth was obtained at sulfur concentrations of from 2 to 30 mM. Initial doubling times were ca. 1.5 h and substrate independent over the range of sulfur concentrations tested. In the cultures with the highest sulfur concentrations, cell numbers increased by greater than 400-fold after 48 h, reaching a maximum density of 6.8 x 10(8) cells ml-1. Yields were determined as total cell carbon and ranged from 1.7 to 5.9 g of C mol of S(0) consumed-1 in the presence of the amino acid supplement and from 0.9 to 3.4 g of C mol of S(0-1) in its absence. Several lines of evidence indicate that cell-to-sulfur contact is not required for growth. Approaches for the culture of sulfur-metabolizing bacteria and potential ecological implications of sulfur reduction in Shewanella-like heterotrophs are discussed.

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Section: Discussionmentioning

confidence: 99%

Growth of the facultative anaerobe Shewanella putrefaciens by elemental sulfur reduction

Moser

Nealson

1996

Appl Environ Microbiol

123

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“…One additional reason for their ability to spoil food seems to be their capacity to produce off-flavoring volatile compounds such as NH3, trimethylamine (TMA), and sulfides (18,25). Strains of A. putrefaciens can be distinguished from strains of Pseudomonas putrefaciens by their lower guanine-plus-cytosine (G + C) content, which has a mean value of about 49 and 58 mol%, respectively (21).…”

mentioning

confidence: 99%

Amino acid and lactate catabolism in trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735

Ringø¹,

Stenberg²,

Strøm³

1984

Appl Environ Microbiol

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The nonfermentative Alteromonas putrefaciens NCMB 1735 grew anaerobically in defined media with trimethylamine oxide as external electron acceptor. All amino acids tested, except taurine and those with a cyclic or aromatic side chain, were utilized during trimethylamine oxide-dependent anaerobic growth. Lactate, serine, and cysteine (which are easily converted to pyruvate) and glutamate and aspartate (which are easily converted to tricarboxylic acid cycle intermediates) were metabolized at the fastest rate. Growth with lactate as growth-limiting substrate gave rise to the formation of 40'mol% acetate, whereas serine and cysteine were nearly completely oxidized to CO2. Molar growth yields with the latter substrates were the same and were 50% higher than with lactate. This showed that more ATP was formed when acetyl coenzyme A entered the, tricarboxylic acid cycle than when it was converted via acetyl phosphate to acetate. Also, growth with formate as substrate indicated that the reduction of trimethylamine oxide to trimethylamine was coupled with energy conservation by a respiratory mechanism.

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“…It is a common psychrotrophic spoilage organism with pink colonies; it degrades cysteine, and under anaerobic conditions H,S is released with obvious effects on both meat color and odor (Gill and Newton 1979;Ringo et al 1984). It is associated with spoilage of red meats, poultry, and fish (Gallo et al 1988;Gill and Tan 1980;Gill 1983;Jsrgensen and Huss 1989;Mast and Stephens 1973;McMeekin and Patterson 1975;Parker and Levin 1983;Seelye and Yearbury 1979;Viehweg et al 1989). No previous studies of the radiation resistance of S. putrefaciens were found.…”

Section: Introductionmentioning

confidence: 99%

INACTIVATION OF SHEWANELLA PUTREFACIENS BY GAMMA IRRADIATION OF RED MEAT AND POULTRY

Thayer

Boyd

1996

Journal of Food Safety

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The resistance of Shewanella putrefaciens ATCC 8071, 8072, and 8073 to gamma radiation was determined in the presence and absence of air on mechanically deboned chicken meat (MDCM). The presence or absence of air (oxygen) did not significantly influence resistance to gamma radiation at 5C, and it was very sensitive with a D10 value of 0.11 ± 0.002 kilogray (kGy) on MDCM. A high percentage of cells surviving irradiation were shown by impedance measurements to have suffered injury. The bacteria were significantly more resistant to gamma radiation at temperatures below the freezing point. At a dose of 0.8 kGy lowering the temperature of irradiation by 10 degrees increased the survival of this food spoilage organism by a 1.66 log10. The type of meat (hamburger, ground beef round, ground pork, and ground turkey breast) did not significantly alter resistance of S. putrefaciens to gamma radiation under identical conditions (D10 value = 0.18 ± 0.01 kGy). The minimum radiation dose currently approved for poultry in the USA, 1.5 kGy, should eliminate S. putrefaciens from meats.

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Relative Incidence of Alteromonas putrefaciens and Pseudomonas putrefaciens in Ground Beef

Cited by 14 publications

References 24 publications

Growth of the facultative anaerobe Shewanella putrefaciens by elemental sulfur reduction

Growth of the facultative anaerobe Shewanella putrefaciens by elemental sulfur reduction

Amino acid and lactate catabolism in trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735

INACTIVATION OF SHEWANELLA PUTREFACIENS BY GAMMA IRRADIATION OF RED MEAT AND POULTRY

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