Relative Quantification of Canine CD56 mRNA Expression by Real-time Polymerase Chain Reaction Method in Normal Tissues and Activated Lymphocytes

Hoshino, Yuki; Tajima, Motoshi; Takagi, Satoshi; Osaki, Tomohiro; Okumura, Masahiro; Fujinaga, Toru

doi:10.1292/jvms.70.309

Cited by 3 publications

(2 citation statements)

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“…RNA was extracted by using RNeasy Mini Kits (Qiagen, Les Ulis, France) according to the manufacturer's instructions. cDNA was synthesized according to a previously reported method [21]. Relative quantification was performed by a previously reported method in order to determine the level of CD56 mRNA expression [21].…”

Section: Comparison Of Pbmcs and Activated Lymphocytesmentioning

confidence: 99%

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Phenotypic Analysis and Effects of Sequential Administration of Activated Canine Lymphocytes on Healthy Beagles

et al. 2008

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ABSTRACT. We attempted to accumulate the basic data for evaluation of activated lymphocyte therapy for small animal medicine. The peripheral blood mononuclear cells (PBMCs) collected from healthy dogs were activated using anti-CD3 antibody and human recombinant (hr) interleukin (IL)-2 and reactivated using hr interferon (IFN)-α and hr IL-2. The property of obtained cells was compared with PBMCs. The number of cells was shown to have increased approximately > 50 -fold by cultivation. The proportion of CD8 + cells was significantly increased, the cytotoxicity of the cultured cells was revealed to have been reinforced. Additionally, CD56 mRNA levels tended to have increased. The cells obtained by this method were confirmed to be activated lymphocytes. Furthermore, we investigated the effects of sequential administration of the obtained cells to healthy dogs. By sequential administration of the activated lymphocytes, the cell proliferative activity, proportion of CD4 + cells and CD8 + cells, and serum IFN-γ concentration were shown to have increased, and no severe adverse effects were observed. Consequently, activated lymphocytes could be induced using anti-CD3 antibody and IL-2 in healthy dogs, and sequential administration of activated lymphocytes reinforced the recipient's immunity.

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Section: Comparison Of Pbmcs and Activated Lymphocytesmentioning

confidence: 99%

“…In previous reports, canine CD56-one of the markers of human NK cells [37]-mRNA increased in stimulated lymphocytes [4,21]. The usage of relative quantification CD56 mRNA method was investigated during activated lymphocyte therapy in this study.…”

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confidence: 93%