Relaxin elicits renoprotective actions accompanied by increasing bile acid levels in streptozotocin-induced diabetic mice

Leo, Chen Huei; Ou, Jamie Li Min; Ong, Eng Shi; Qin, Cheng Xue; Ritchie, Rebecca H.; Parry, Laura J.; Ng, Hooi Hooi

doi:10.1016/j.biopha.2023.114578

Cited by 9 publications

(2 citation statements)

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“…The common features of metabolic syndrome include abdominal obesity, atherogenic dyslipidemia, hypertension, and insulin resistance accompanied by hyperglycemia, which contributes to oxidative stress, a pro-inflammatory and prothrombotic condition [31,50]. Cardiovascular disease, type 2 diabetes, and fatty liver are some of the comorbidities linked to the metabolic syndrome [50].…”

Section: Transcriptomic Profiling and Molecular Signatures Of Mpe Tre...mentioning

confidence: 99%

“…As green extraction methods are more environmentally friendly to organic solvents, some of the earlier studies have determined the extraction yield and efficiency of phytochemicals (e.g., α-Mangostin) extracted from the mangosteen pericarp [28,29]. However, the effect of these extracts on the cellular targets and their associated molecular signatures remains unclear in endothelial cells, which are important regulators of vascular homeostasis [30,31]. Thus, the aim of the present study is to use PHWE to extract phytochemical compounds from mangosteen pericarp and determine the biological effects of mangosteen pericarp extract (MPE) in endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

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Pressurized Hot Water Extraction of Mangosteen Pericarp and Its Associated Molecular Signatures in Endothelial Cells

Tan,

Shanmugham,

Chin

et al. 2023

Antioxidants

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The mangosteen (Garcinia mangostana L.) pericarp is known to be rich in potent bioactive phytochemical compounds such as xanthones, which possess pharmacologically important antioxidant activity and beneficial cardiometabolic properties. Mangosteen pericarp is typically classified as unavoidable food waste and discarded, despite being rich in bioactive phytochemical compounds that therefore present an exciting opportunity for valorization. Thus, this study aims to extract phytochemical compounds from mangosteen pericarp using pressurized hot water extraction (PHWE) and determine its biological effects in endothelial cells using RNA sequencing. Liquid chromatography with MS/MS (LC/MSMS) and UV detection (LC/UV) was subsequently used to identify three key phytochemical compounds extracted from the mangosteen pericarp: α-Mangostin, γ-Mangostin, and Gartanin. Within the tested range of extraction temperatures by PHWE, our results demonstrated that an extraction temperature of 120 °C yielded the highest concentrations of α-Mangostin, γ-Mangostin, and Gartanin with a concomitant improvement in antioxidant capacity compared to other extraction temperatures. Using global transcriptomic profiling and bioinformatic analysis, the treatment of endothelial cells with mangosteen pericarp extracts (120 °C PHWE) for 48 h caused 408 genes to be differentially expressed. Furthermore, our results demonstrated that key biological processes related to “steroid biosynthesis and metabolism”, likely involving the activation of the AMPK signaling pathway, were upregulated by mangosteen pericarp extract treatment. In conclusion, our study suggests a green extraction method to valorize phytochemical compounds from mangosteen pericarp as a natural product with potential beneficial effects on cardiometabolic health.

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Section: Transcriptomic Profiling and Molecular Signatures Of Mpe Tre...mentioning

confidence: 99%