Release of enzymes by plant tissue cultures

Straus, Jacob; Campbell, William A.

doi:10.1016/0024-3205(63)90037-7

Cited by 41 publications

(15 citation statements)

References 11 publications

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“…Working with different plant organs and tissue cultures, Straus (1962) demonstrated the presence of a cell wall-located sucrose-hydrolyzing system in at least one strain of tobacco cultures. Later work from the same laboratory (Straus and Campbell, 1963) showed that the cell walls of a variety of tissue cultures were able to release enzymes such as phosphatase, amylase, and peroxidase into the medium. The major metabolic role of the cell wall is, however, considered to be its sucrose-hydrolyzing capacity due to the presence of the enzyme invertase (EC 3.2.1.26).…”

Section: Role Of the Cell Wallmentioning

confidence: 97%

The Metabolism and Utilization of Carbohydrates by Suspension Cultures of Plant Cells

Stepan-Sarkissian

Fowler

1986

Carbohydrate Metabolism in Cultured Cells

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Section: Role Of the Cell Wallmentioning

confidence: 97%

The Metabolism and Utilization of Carbohydrates by Suspension Cultures of Plant Cells

Stepan-Sarkissian

Fowler

1986

Carbohydrate Metabolism in Cultured Cells

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“…Subsequent work with virusinfected and also with healthy cells showed that peroxidase, acid phosphatase, indoleacetic acid oxidase, amylase and glucanase were present in nutrient media of numerous species [11,16,21,22,26]. Despite these early findings and in contrast to microbial and animal cell cultures, more recent studies on the distribution of enzymic activities in plant cell cultures are limited [10,29,30].…”

Section: Introductionmentioning

confidence: 99%

Isozymes in nutrient medium of suspension-cultured apple cells (Malus sylvestris Mill.)

Berger

Drawert

Kunz

1988

Plant Cell Tiss Organ Cult

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The time course of the activities of esterase, ct-galactosidase, and fl-glucosidase in cell sap and nutrient medium in in vitro cultured apple cells (Malus sylvestris Mill.) was studied.The corresponding isozyme patterns and the intracellular and extracellular isozyme patterns of acid phosphatase and polyphenol oxidase were compared using isozyme visualization methods adapted to ultra-thin-layer isoelectric focusing. Neither quantitative (total activity) nor qualitative (isozyme pattern) data were congruent for cell saps and nutrient media. Malate dehydrogenase, malic enzyme, and glutamate dehydrogenase occurred in cell sap only. The extracellular activities probably originate to a great part from a programmed release by intact cells. Nutrient media of plant cell cultures constitute a rich source of active plant isozymes.

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“…Of particular relevance to the present work is a report (36) of the secretion of peroxi(lase, aci(l phosphatase, indolylacetic aci(l oxidase, and amylase into the culture media bathing plant tissue cultures. In addition, reports have appeared (2,6,14) of the occurrence of an exocellular invertase, possibly involved in sucrose metabolism by roots.…”

mentioning

confidence: 97%

Exocellular Enzymes of Corn Roots

Chang

Bandurski

1964

Plant Physiol.

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Little attention has been given to the occurrence of exocellular hydrolytic enzymes associated with the roots of higher plants. Such enzymes, if present, could solubilize macromolecular constituents of organic soils, permitting uptake and utilization by the plant of the resultant low molecular weight organic compounds. The present work supports this concept by demonstrating that several exocellular hydrolytic enzymes are associated with the roots of intact corn seedlings. Distinction has been made between exocellular enzymes released into the culture media and enzymes apparently bound to the root surface.A previous paper from this laboratory (17) reviewedl earlier studies and presented evidence for the occurrence of exocellular enzymnes associated with the primary cell w all of corn coleoptiles. Of particular relevance to the present work is a report (36) of the secretion of peroxi(lase, aci(l phosphatase, indolylacetic aci(l oxidase, and amylase into the culture media bathing plant tissue cultures. In addition, reports have appeared (2, 6, 14) of the occurrence of an exocellular invertase, possibly involved in sucrose metabolism by roots.In the present work it is reporte(I that invertase, cellobiase, adenosine tri phosphatase, pyrophosphatase, ancl nuclease activity is observed when appropriate substrates are added to nutrient solution bathing the roots of intact seedling corn plants. In addition, invertase and nuclease are (letectecl as soluble enzymes in the nutrient solution. Materials and MethodsCorn seeds (Zea ways L. var. Michigan hybrid 350) were soaked in running water overnight, then surface sterilized by soaking in 1% sodium p-toluenesulfonchloramide (chlorazenie) for 5 minutes and finally, washed thoroughly wxith sterile glass distilled water. The soaked seeds were germinate(l between sterilized paper towels in a moist chamber at room temperature an(l used at an age of 4 to 5 days.Two incubation methods were used, A) the substrate was addle(l to the nutrient in which the seedling roots were growing, an(l B) the seedlings were removed from the nutrient and the substrate then added. MWethod A measured total enzymatic activity of the roots while method BP measure(l activity of enzymes present in solution. 60For procedure A, 8 intact seedlings, each 80 mml in length, were washed with glass distilled water, then incubated at room temperature in 15 X 100 mm tubes. The tubes contained seedlings, substrate, and necessary cofactors, in 13 nml of inorganic nutrient solution (37). Aseptic air was supplied at the bottom of the tube by means of a fine glass tube. At intervals, 1 nl of reaction mixture was removed an(l the amount of substrate hydrolyzed(l determined by standard methods with minor modifications.For procedure BP 8 seedlings were suspended in tubes containing 13 nml of inorganic nutrient solution. Samples of 1 nml of the nutrient were removed from the tubes, at varying time intervals, and incubated with the appropriate substrate and cofactors. Rates of reaction were determined as for B above.No(direct...

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Release of enzymes by plant tissue cultures

Cited by 41 publications

References 11 publications

The Metabolism and Utilization of Carbohydrates by Suspension Cultures of Plant Cells

The Metabolism and Utilization of Carbohydrates by Suspension Cultures of Plant Cells

Isozymes in nutrient medium of suspension-cultured apple cells (Malus sylvestris Mill.)

Exocellular Enzymes of Corn Roots

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