The Metabolism and Utilization of Carbohydrates by Suspension Cultures of Plant Cells

Promotion of fungal growth and underlying physiochemical changes of polysaccharides in Rigidoporus ulmarius, an edible Basidiomycete mushroom

“…This suggests that fungal growth of R. ulmarius was sensitive to both sucrose and glucose. This could be due to sucrose playing a balancing role in cell growth through hydrolysis by invertase and sucrose synthase, and the resultant hexose enters the glycolytic and pentose phosphate pathways (Stepan-Sarkissian & Fowler, 1986). Tang, Zhu, Li, Mi, and Li (2008) reported that during the submerged fermentation of the mushroom, Tuber sinense (Chinese truffle), in shaking flasks, sucrose, glucose, and fructose were identified as the three best carbon sources for mycelial growth and polysaccharide production.…”

Enhancement of polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense by optimization of medium compositions and feeding of sucrose

“…3 also showed that PLB growth in medium with sucrose was higher than that in the medium with glucose or fructose. This reason might be that sucrose could supply a balanced carbon source for cell growth by hydrolysis of invertase and sucrose synthase, and the resulted hexose can directly participate in glycolytic and pentose phosphate pathways [27,28].…”

Synthesis of saikosaponins in adventitious roots of Bupleurum chinense by semi-continuous culture

“…In plant tissue culture medium, sugars (sucrose in particular) are the most predominant components, and play a critical role as osmoticums as well as carbon sources (Sarkissian and Fowler 1986;Conner and Falloon 1993). Supplementation of sucrose (29-88 mM) to culture media under both ambient and elevated CO 2 conditions led to a reduction in shoot and root performance of in vitro plantlets (Suriyan et al 2011).…”