Release of gentamicin from a tricalcium phosphate bone implant

Silverman, Lance D.; Lukashova, Lyudmila; Herman, Oren T.; Lane, Joseph M.; Boskey, Adele L.

doi:10.1002/jor.20284

Cited by 65 publications

(38 citation statements)

References 24 publications

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“…35 This implant surface with a growth factor coating not only offers the advantage of a high localized drug concentration but is also an effective way of delivering growth factors directly to the percutaneous site. Several previous studies have tried to fabricate implant surfaces with growth factor coatings by adsorbing antibiotics or growth factors directly to the implant surface with bone cements 36,37 or by loading agents in collagen sponges, porous coatings, or polymer-based matrices. 38,39 However, all of these approaches have shortcomings, which include chemical instability and local inflammatory reactions due to material composition.…”

Section: Discussionmentioning

confidence: 99%

Increased fibroblast functionality on CNN2-loaded titania nanotubes

Wei¹,

Wu²,

Feng³

et al. 2012

IJN

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Infection and epithelial downgrowth are major problems associated with maxillofacial percutaneous implants. These complications are mainly due to the improper closure of the implant-skin interface. Therefore, designing a percutaneous implant that better promotes the formation of a stable soft tissue biologic seal around percutaneous sites is highly desirable. Additionally, the fibroblast has been proven to play an important role in the formation of biologic seals. In this study, titania nanotubes were filled with 11.2 kDa C-terminal CCN2 (connective tissue growth factor) fragment, which could exert full CCN2 activity to increase the biological functionality of fibroblasts. This drug delivery system was fabricated on a titanium implant surface. CCN2 was loaded into anodized titania nanotubes using a simplified lyophilization method and the loading efficiency was approximately 80%. Then, the release kinetics of CCN2 from these nanotubes was investigated. Furthermore, the influence of CCN2-loaded titania nanotubes on fibroblast functionality was examined. The results revealed increased fibroblast adhesion at 0.25, 0.5, 1, 2, 4, and 24 hours, increased fibroblast viability over the course of 5 days, as well as enhanced actin cytoskeleton organization on CCN2-loaded titania nanotubes surfaces compared to uncoated, unmodified counterparts. Therefore, the results from this in vitro study demonstrate that CCN2-loaded titania nanotubes have the ability to increase fibroblast functionality and should be further studied as a method of promoting the formation of a stable soft tissue biologic seal around percutaneous sites.

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Section: Discussionmentioning

confidence: 99%

Increased fibroblast functionality on CNN2-loaded titania nanotubes

Wei¹,

Wu²,

Feng³

et al. 2012

IJN

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“…Whilst the chosen parameters are intended to mimic an in-vivo scenario in terms of quantity of material used, volume of eluent, and eluent exchange, the model does not take into account factors such as clot formation, which will have an effect on the diffusion of antibiotic into the surgical site, and protein binding [51], which will affect the antimicrobial efficacy of the antibiotics released into the extracellular matrix. Silverman et al [52] attempted to mimic the in-vivo environment by determining the effect of clot formation around gentamicin-impregnated b-tricalcium phosphate granules on antibiotic elution, with results showing that clotted blood slowed drug release.…”

Section: Discussionmentioning

confidence: 99%

Local Release of Antibiotics for Surgical Site Infection Management Using High-Purity Calcium Sulfate: An In Vitro Elution Study

Aiken

Cooper

Florance

et al. 2015

Surgical Infections

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Background: The aim of this study was to characterize the elution of four antibiotics from pharmaceuticalgrade calcium sulfate beads and show that the eluted antibiotics retained efficacy. Methods: Calcium sulfate was combined with gentamicin, tobramycin, vancomycin, or rifampicin (ratio: 20 g of calcium sulfate, to 240 mg, 500 mg, 900 mg, and 600 mg of antibiotic, respectively). Three grams of beads were immersed in 4 mL of sterile phosphate-buffered saline (PBS) at 37°C. At each time point (4, 8, 24 h; 2, 7, 14, 28, 42 d), eluates were removed for analysis by liquid chromatography-mass spectrometry. The antimicrobial efficacy of antibiotics combined with calcium sulfate beads after 42 d was tested by a modified KirbyBauer disc diffusion assay. Results: All samples showed a generally exponential decay in the eluted antibiotic concentration. At the first time point, both gentamicin and tobramycin had eluted to a peak concentration of approximately 10,000 mcg/mL. For rifampicin, the peak concentration occurred at 24 h, whereas for vancomycin, it occurred at 48 h. The eluted concentrations exceeded the minimum inhibitory concentration for common periprosthetic joint infection pathogens for the entire span of the 42 study days. Mass spectrometry confirmed all antibiotics were unchanged when eluted from the calcium sulfate carrier. Antimicrobial efficacy was unaltered after 42 d in combination with calcium sulfate at 37°C. Conclusions: Pharmaceutical-grade calcium sulfate has the potential for targeted local release of tobramycin, gentamicin, vancomycin, and rifampicin over a clinically meaningful time period.

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“…Hydroxyapatite [Ca 10 (PO 4 ) 6 (OH) 2 : HAP] is one of the attractive biomaterials due to its similarity in properties of bone that includes chemical composition, biodegradability, bioactivity, and osteoconductivity.…”

Section: Introductionmentioning

confidence: 99%

Densification behavior of hydroxyapatite green pellets prepared by different methods

Kim

Yanagisawa

Onda

et al. 2015

J. Ceram. Soc. Japan

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The densification behavior of the green pellets with different densities prepared from hydroxyapatite powders consisting of plate-like particles was investigated. The green pellets were prepared by two methods, hydrothermal hot-pressing and uniaxial pressing, at different compression pressures. The hydrothermal hot-pressing gave the green pellets with densities higher than those using with uniaxial pressing. The shrinkage of the green pellets was observed by calcination at temperature above 800°C. The green pellets with higher bulk density gave the sintered compacts with higher density. When the calcination temperature was increased, densification proceeded with the increase in pore diameter and the decrease of pore volume. Even at temperature below 700°C, the size of pores increased with the increase in calcination temperature without shrinkage of the green pellets. Thus, the green compacts of the same density but with different pore sizes distribution prepared by hydrothermal hot-pressing and uniaxial pressing, had similar pore size distribution after calcination at 700°C, As a result, they had the same densification behavior by calcination at high temperature above 800°C. The densification behavior was found to be dependent on the density of green pellets, but not on the method of preparation of green pellets.

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Release of gentamicin from a tricalcium phosphate bone implant

Cited by 65 publications

References 24 publications

Increased fibroblast functionality on CNN2-loaded titania nanotubes

Increased fibroblast functionality on CNN2-loaded titania nanotubes

Local Release of Antibiotics for Surgical Site Infection Management Using High-Purity Calcium Sulfate: An In Vitro Elution Study

Densification behavior of hydroxyapatite green pellets prepared by different methods

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