Release of lipoxygenase products of arachidonic acid from freshly isolated human keratinocytes

Grabbe, Jürgen; Czarnetzki, Beate M.; Mardin, Mithat

doi:10.1007/bf00511071

Cited by 47 publications

(6 citation statements)

References 13 publications

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“…Other reports have found higher amounts of LTB4 released from human alveolar macrophages than from human peripheral PMN's (24). Human airway epithelium has a synthesizing capacity for LTB4 (19), and keratinocytes have also been demonstrated to synthesize LTB4, though in smaller amounts (18). LTB4 synthesis was stimulated during a specific allergic reaction in vitro (25), although this could not be confirmed in vivo (8).…”

Section: Discussionmentioning

confidence: 99%

Chemotactic Activity of LTB₄ in Man

Bisgaard¹,

Helqvist²,

Boudet³

et al. 1986

Allergy

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An improved skin window chamber technique has been developed and used for a quantitative study of the chemotactic effect of leukotriene B4 (LTB4). LTB4 (0.5 microM) was exposed to a skin window on the forearm of eight healthy volunteers, while phosphate buffered saline served as control in a skin window on the other forearm. Skin window exudates and samples of blood draining the skin window areas were collected after 1, 2, 4, 8, and 24 h. The samples were quantitated for the different types of leukocytes as well as the intra- and extracellular concentration of the eosinophilic cationic protein and lactoferrin as markers of eosinophil and neutrophil granulocytes. A significantly increased migration of neutrophil granulocytes into the skin window chamber containing LTB4 was found from the 2nd to the 8th hour after the initial LTB4 exposure. The eosinophils reached a significant peak at the 4th hour. The rise in the actual number of eosinophil cells did not reach significance, whereas measurements of the eosinophilic cationic protein in the cellular fraction of the exudate exhibited a significant increase as a reflection of the number of eosinophils. This highlights the potential clinical value of eosinophilic cationic protein measurements to reveal eosinophilia instead of the traditional eosinophil counts. Extracellular eosinophilic cationic protein and lactoferrin did not change significantly in the LTB4-exposed skin window, implying that LTB4 does not activate the eosinophils and neutrophils to exocytosis of their enzymes. The present in vivo results support the concept of LTB4 being a potent chemoattractant to neutrophil and less so to eosinophil granulocytes in humans, a chemoattractant that recruits the leukocytes but does not seem to activate them.

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Section: Discussionmentioning

confidence: 99%

Chemotactic Activity of LTB₄ in Man

Bisgaard¹,

Helqvist²,

Boudet³

et al. 1986

Allergy

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“…LTA 4 can be further conjugated to GSH and generate a series of peptido-leukotrienes, or, following transcelluar metabolism, contribute to the formation of lipoxins (LX) [65]. Cutaneous 5-LOX activity is rather low and has been associated mainly with epidermal keratinocytes [66,67], Langerhans cells [68,69] and infiltrating leukocytes [65]. Interestingly, 5-LOX activity is increased when keratinocytes differentiate [70].…”

Section: Lipoxygenase-derived Mediatorsmentioning

confidence: 99%

Eicosanoids in skin inflammation

Nicolaou

2013

Prostaglandins, Leukotrienes and Essential Fatty Acids

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SummaryEicosanoids play an integral part in homeostatic mechanisms related to skin health and structural integrity. They also mediate inflammatory events developed in response to environmental factors, such as exposure to ultraviolet radiation, and inflammatory and allergic disorders, including psoriasis and atopic dermatitis. This review article discusses biochemical aspects related to cutaneous eicosanoid metabolism, the contribution of these potent autacoids to skin inflammation and related conditions, and considers the importance of nutritional supplementation with bioactives such as omega-3 and omega-6 polyunsaturated fatty acids and plant-derived antioxidants as means of addressing skin health issues.2

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“…9 Murine keratinocytes have been shown to produce LTB4. 10 However, studies in human keratinocytes remain mixed with one study that showed a lack of expression of 5-LOX or the ability for endogenous LTB4 production, 11 while others noted expression of 5-LOX in the epidermis 12 and documented LTB4 13 and 5-HETE release. 13 Regardless, keratinocytes can produce LTB4 in the presence of LTA4 as they express the LTA4 hydrolase necessary for the synthesis of LTB4.…”

Section: Overview Of Eicosanoidsmentioning

confidence: 99%

Eicosanoids and Keratinocytes in Wound Healing

Sivamani

2014

Advances in Wound Care

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Release of lipoxygenase products of arachidonic acid from freshly isolated human keratinocytes

Cited by 47 publications

References 13 publications

Chemotactic Activity of LTB₄ in Man

Chemotactic Activity of LTB₄ in Man

Eicosanoids in skin inflammation

Eicosanoids and Keratinocytes in Wound Healing

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Release of lipoxygenase products of arachidonic acid from freshly isolated human keratinocytes

Cited by 47 publications

References 13 publications

Chemotactic Activity of LTB4 in Man

Chemotactic Activity of LTB4 in Man

Eicosanoids in skin inflammation

Eicosanoids and Keratinocytes in Wound Healing

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Product

Resources

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Chemotactic Activity of LTB₄ in Man

Chemotactic Activity of LTB₄ in Man