Relevance of charge balance and hyaluronic acid on alginate‐chitosan sponge microstructure and its influence on fibroblast growth

Abstract

The study of biomaterials by electrical charge scaling to explore the role of net charge on biocompatibility and suitability for tissue regeneration has been limited as has the search for products that could improve this first-rate variable. In the present study, we prepared sponges composed of chitosan/alginate (CS/ALG) with or without hyaluronic acid (HA) by mixing polymer stock solutions of different net electric charge ratios (n(+/) n(-) ), and then lyophilizing them to obtain porous materials. The electric charge ratios n(+/) n(-) studied were 0.3, 0.8, 1.0, and 2.5 for CS/ALG and 0.3, 1.0, 1.9, and 3.7 for CS/ALG/HA sponges. Under these conditions a role for net electric charge balance over sponge microstructure rearrangement, protection to dissolution, cellular proliferation, and cell-cell interactions was apparent, effects that were enhanced by copolymer modification with HA. Mass balance, electric charge, and specific products that influence both such as HA, have a potential in biomaterials for wound healing. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2537-2543, 2016.

Hyaluronic acid—Based wound dressings: A review

“…Such structural variation is responsible for enhancing the O 2 supply, nutritional flow and cell proliferation at the wound site (Bružauskaitė, Bironaitė, Bagdonas, & Bernotienė, 2016). Moreover, the cell proliferation rate has increased when they were incubated with sponges containing HA (Orellana et al, 2016).…”

Combining Materials Obtained by 3D-Printing and Electrospinning from Commercial Polylactide Filament to Produce Biocompatible Composites

“…Although we used a commercial 3D-printer filament to print the scaffolds, in all the composites cultured with 3T3 cells we observed cells attached to the main 3D-printed structure (Figure 13A,B), showing the potential of commercial PLA to produce biocompatible materials [62]. The morphology of cells found spread over the surface of 3D-printed pieces of the composite are a blend between some apoptotic cells showing a round shape and a shrunk nucleus, besides a well-formed and interconnected network of fibroblasts linked by polarized filipodia protrusions [63][64][65]. Some cells are found in the fibrous layer of the composites after 5 days of culture (Figure 13C,D).…”

Aerogels made of chitosan and chondroitin sulfate at high degree of neutralization: Biological properties toward wound healing

“…Although the nanoparticles show a negative zeta potential that ensures their stability, most of the polymeric mass is confined in the nanoparticles, so that only a slight excess of one of the charged components is responsible for the zeta potential observed. Studies on proliferation of fibroblasts in sponges containing a high excess of negative polymeric charges show low rate of cellular growth, but after neutralization with cationic charges such as provided by CS the cells are stimulated to proliferation . As mentioned, the molecular assembling based in electrostatic forces also avoids the use of cross‐linkers, frequently related with toxic and allergic reactions .…”

“…Studies on proliferation of fibroblasts in sponges containing a high excess of negative polymeric charges show low rate of cellular growth, but after neutralization with cationic charges such as provided by CS the cells are stimulated to proliferation. 29 As mentioned, the molecular assembling based in electrostatic forces also avoids the use of cross-linkers, frequently related with toxic and allergic reactions. 30 By other side, the low density of the materials could also contribute to minimizes the chances of adverse reactions.…”