Relevance of Oxygen Free Radicals and Antioxidants in Sperm Production and Function

Ogbuewu, I. P.; Aladi, N. O.; Etuk, I. F.; Opara, M. N.; Uchegbu, M. C.; Okoli, I. C.; Iloeje, M. U.

doi:10.3923/rjvs.2010.138.164

Cited by 33 publications

(33 citation statements)

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“…The fish oil in the two groups stored at AT persistent 135 days, which may aggravate lipid oxidation further. The oxidized fish oil would damage normal cells, cause liver inflammation, impede hepatocyte to assimilate exogenous fatty acids, and then enhance decomposition of glycogen (Ogbuewu et al, ). Interestingly, TCHOL in serum of fish fed with T AT+135d+EQ and T AT+135d+HEQ had no significant differences with fish fed with T F+EQ and T F .…”

Section: Discussionmentioning

confidence: 99%

Effects of fish oil with difference oxidation degree on growth performance and expression abundance of antioxidant and fat metabolism genes in orange spotted grouper,Epinephelus coioides

et al. 2018

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The present study was designed to assess the effects of fish oil with different oxidation degree on growth performance, serum biochemistry parameters and expressive abundance of oxidative stress and fat metabolism genes of orange spotted grouper Epinephelus coioides. The oxidized fish oil was conducted as follows: storage temperature: 4°C, ambient temperature (AT, [31.5 ± 3.5]°C); storage time: 45, 90, 135 days; antioxidant contents: 30 mg/kg (ethoxyquin [EQ]), 300 mg/kg Higher EQ (HEQ). According to the different treated conditions, 14 kinds of fish oil with different oxidation degree were obtained: T F+EQ [positive control (fresh oil + EQ)],T AT+135d+EQ , T AT+135d+HEQ . Groupers were fed isonitrogenous and isolipidic diets containing 14 kinds of fish oil for 8 weeks, respectively. The results showed that survival, weight gain rate and thermal growth coefficient decreased as oxidation degree of dietary fish oil increased (p < 0.05). Higher serum total protein, triglyceride and glucose were observed with ascending oxidation degree of fish oil (p < 0.05). The genes expression levels of catalase, superoxide dismutase and glutathione peroxidase were up-regulated with dietary oxidized level increasing (p < 0.05). In addition, the similar status also appeared in expression of peroxisome proliferator-activated receptor gamma (PPARγ), hormone-sensitive lipase (HSL) and fatty acid synthase (FAS) genes. In conclusion, the fish oil would show negative influence on the fish health until peroxide value and p-anisidine value in oil exceed 12.96 meq/kg and 20.89. The best storage condition for fish oil is 4°C, 45 days and 30 mg/kg EQ which could keep fish oil available property to grouper. K E Y W O R D Santioxidant, fat metabolism, grouper, growth, oxidized fish oil

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Section: Discussionmentioning

confidence: 99%

Effects of fish oil with difference oxidation degree on growth performance and expression abundance of antioxidant and fat metabolism genes in orange spotted grouper,Epinephelus coioides

et al. 2018

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“…Main effects of cold shock on sperm cells are able to reduce motility and viability, changes in permeability and lipid components in the membrane. The numbers of motile undergo reduction accompanied by enzyme release, ion movement through membrane, and changes in lipid contents such as phospholipids and cholesterol that play significant roles in maintaining the integrity of plasma membrane, as well as reduced capability of sperm cells in controlling Ca 2+ movement (Ogbuewu et al, 2010).…”

Section: Decreasing Of Pasundan Bull Semen Quality During Freezingmentioning

confidence: 99%

“…Although different from AndroMed ® , TS can be used as semen diluents in the future because it also contains soy lecithin. Lecithin extracted from soybeans is appropriate choice for lecithin of semen diluents in the future (Aires et al, 2003) and able to suppress oxidative stress (Ogbuewu et al, 2010), but further research is required to obtain buffer composition with similar quality to AndroMed ® .…”

Section: Decreasing Of Pasundan Bull Semen Quality During Freezingmentioning

confidence: 99%

Freezing Capability of Pasundan Bull Sperm Using Tris-Egg Yolk, Tris-Soy, and Andromed® Diluents

Baharum¹,

Arifiantini²,

Yusuf³

2017

J Kedokt Hewan

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The aims of this study were to investigate the freezing capability of Pasundan bull spermatozoa in Tris-egg yolk (TEY), Tris-soy (TS), and AndroMed ® as diluents. Semen were collected twice a week from four Pasundan bulls aged 3-5 years old using an artificial vagina and evaluated macro-and microscopically. Semen had ≥70% sperm motility, ≥800x10 6 /mL sperm concentration, and less than 20% sperm abnormalities were divided into three parts and each of them diluted with TEY, TS, or AndroMed ® . After an equilibration step at 5° C for four hours, diluted semen were packaged in 0.25 mL straw, frozen in liquid nitrogen for ten minutes and kept in liquid nitrogen container until examination. Motility test on fresh, diluted, equilibrated, and after-thawed semen was done using Androvision ® . The results showed that after thawing motility of sperm diluted in AndroMed ® (58.64±0.72%) was higher than in TEY (49.45±1.22%) and TS (39.34±6.33%). Sperm motility of Pasundan bulls diluted in these three diluents reduced around 33.27±2.45% during freezing process. ____________________________________________________________________________________________________________________ ABSTRAKPenelitian ini bertujuan mengetahui kemampuan bertahan spermatozoa sapi pasundan dalam proses pembekuan menggunakan pengencer tris kuning telur (TKT), tris soya (TS), dan AndroMed ® . Empat ekor pejantan sapi pasundan dengan umur sekitar 3-5 tahun dikoleksi semennya menggunakan vagina buatan dua kali dalam seminggu. Semen hasil koleksi segera dievaluasi secara makroskopis dan mikroskopis. Semen dengan motilitas ≥70%, konsentrasi ≥800x10 6 /ml, dan abnormalitas spermatozoa ≤20% dibagi tiga bagian, masing-masing diencerkan menjadi 100x10 6 /ml, menggunakan pengencer TKT, TS, atau AndroMed ® , diekuilibrasi pada suhu 5° C selama empat jam, dan dikemas dalam straw 0,25 ml. Semen yang telah dikemas dibekukan dalam uap nitrogen cair selama sepuluh menit dan disimpan dalam kontainer untuk pengujian lebih lanjut. Pengujian motilitas dilakukan menggunakan Androvision ® pada semen segar, setelah pengenceran, setelah ekuilibrasi, dan post thawing. Hasil penelitian menunjukkan motilitas spermatozoa post thawing dalam pengencer AndroMed ® (58,64±0,72%) lebih tinggi dibandingkan pengencer TS (39,34±6,33%), namun tidak berbeda dengan TKT (49,45±1,22%). Pengencer TKT tidak memperlihatkan perbedaan (P>0,05) dengan TS. Penurunan motilitas selama proses pembekuan pada sapi pasundan adalah sebesar 33,27±2,45%. ____________________________________________________________________________________________________________________ Kata kunci: andromed, freezing capability, sapi pasundan, tris kuning telur, tris soya

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“…However, oxidative stress has been associated with male infertility (Bansal and Bilaspuri, 2010;El-Tohamy, 2012). The toxicity of ROS is responsible for several cellular alterations (Soares et al, 2014) also, productions of ROS decrease sperm motility (Ogbuewu et al, 2010). Because of lipid peroxidation, the plasma membrane decrease fluidity and integrity (Bansal and Bilaspuri, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…ROS can induce sperm cells damage, causing oxidative damage to the cell membrane, starting lipid peroxidation cascade, through of attack of polyunsaturated fatty acids (Ogbuewu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Oxidative Status of Boar Semen during Storage

Zanella¹,

Zanella²,

Poetini³

et al. 2016

American Journal of Biochemistry and Biotechnology

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Abstract:The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 µM. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In conclusion, LT diluent with the addition of antioxidant as Trolox can reduce the oxidative stress in sperm cells, which could be used to protect spermatozoa against oxidative damage, preventing motility losses in swine semen.

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Relevance of Oxygen Free Radicals and Antioxidants in Sperm Production and Function

Cited by 33 publications

References 0 publications

Effects of fish oil with difference oxidation degree on growth performance and expression abundance of antioxidant and fat metabolism genes in orange spotted grouper,Epinephelus coioides

Effects of fish oil with difference oxidation degree on growth performance and expression abundance of antioxidant and fat metabolism genes in orange spotted grouper,Epinephelus coioides

Freezing Capability of Pasundan Bull Sperm Using Tris-Egg Yolk, Tris-Soy, and Andromed® Diluents

Oxidative Status of Boar Semen during Storage

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