Relevance of surface characteristics in the adhesiveness of polymicrobial biofilms to crown restoration materials

Abstract

We used a polymicrobial (PM) biofilm model to examine associations of bacterial adhesiveness with surface characteristics of various dental materials. Four types of dental materials (apatite pellet, zirconia, ceramic, and composite resin) with rough and mirror surfaces were used. Surface roughness, surface free energy, zeta potential, and colony-forming units (CFUs) of the biofilm formations were measured. Biofilms were cultured for 24 h under anaerobic conditions, plated onto blood agar medium, and anaerobically cultured for 4 days. After culturing, CFU per mm 2 was calculated, and samples were observed under a scanning electron microscope. Means and standard deviations of the experimental data were estimated, and one-way ANOVA and Tukey multiple comparison assays were performed. Pearson correlation coefficients were obtained for the CFU and surface characteristics. Surface roughness and surface free energy appeared to affect generation of PM biofilms on oral materials, and zeta potential was involved in generation of PM biofilms on mirrorground oral materials.

Peri-implant marginal bone changes with implant-supported metal-ceramic or monolithic zirconia single crowns: A retrospective clinical study of 1 to 5 years

“…In the present study, the PI of the metal-ceramic group was significantly higher than that of the monolithic zirconia group, possibly because of the antimicrobial activity of zirconia, reported in previous studies. 32,33 Although the PPD and BOP of the metal-ceramic group were higher than those of the monolithic zirconia group, no significant differences were observed, which was consistent with the results of a previous study. 24 Data on peri-implant soft tissue in this study were obtained from the most recent follow-up, and additional follow-up data are needed to verify this result.…”

Influence of Dental Prosthesis and Restorative Materials Interface on Oral Biofilms

“…The receptors of salivary pellicle offer binding sites for floating initial bacteria cells to attach to these surfaces and form microcolonies. As time goes by, the bacteria cells aggregate, proliferate, and grow into a mushroom-shaped mature biofilm, firmly attaching to these surfaces [ 23 , 24 ]. Therefore, bacterial cells within the biofilm do not exist as independent entities but, rather, as a coordinated, metabolically integrated microbial community [ 22 ].…”

Comparative Study on the Degree of Bacterial Biofilm Formation of Dental Bridges Made from Three Types of Materials

“…Saliva-derived pellicles immediately form on the surfaces of thoroughly cleaned teeth and attract bacteria through chemical or electrostatic interactions or by antigens on the surfaces of bacterial cells. The most common organisms present in oral biofilms are the initial colonizer Streptococcus, early colonizer Veillonella, middle colonizer Porphyromonas gingivalis, and Fusobacterium nucleatum [1][2][3][4]. The ability of individual bacteria to generate biofilms increases dramatically in the presence of the Veillonella genus.…”