Reliability of coprological diagnosis of Anoplocephala perfoliata infection

Meana, Aránzazu; Luzón, Mónica; Corchero, J.; Gómez-Bautista, Mercedes

doi:10.1016/s0304-4017(97)00145-3

Cited by 59 publications

(40 citation statements)

References 11 publications

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“…Whilst these tests are inexpensive and demand no sophisticated equipment, they are time consuming, messy and lack sensitivity. Validation studies of various coprological methods have reported sensitivities of 11-61% [38,47].…”

Section: An Immunodiagnostic Assay For Equine Tapeworm Infectionmentioning

confidence: 99%

Recent developments in research into the Cyathostominae and Anoplocephala perfoliata

et al. 2004

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-Intestinal helminths are an important cause of equine disease. Of these parasites, the Cyathostominae are the commonest group that infect horses. These nematodes consist of a complex tribe of 51 species, although individual horses tend to harbour 10 or so common species, in addition to a few rarer species. The Cyathostominae can be extremely pathogenic, and high levels of infection result in clinical symptoms ranging from chronic weight loss to colic, diarrhoea and death. As part of their life cycle, immature cyathostomins penetrate the large intestinal wall, where they can enter a state of inhibited larval development. These larvae can exist in this state for months to years, after which they subsequently re-emerge. If larvae re-emerge in large numbers (i.e. several million), severe pathological consequences ensue. The inhibited larvae are also relatively refractory to several of the currently available anthelmintics, so that horses treated previously with anthelmintics can still carry life-threatening burdens of these parasitic stages. Little is known about the cyathostomin larvae during their mucosal phase, and current research efforts are focused on investigating the biology of these stages. Much of the research described here highlights this area of research and details studies aimed at investigating the host immune responses that the mucosal larvae invoke. As part of this research effort, molecular tools have been developed to facilitate the identification of larval and egg stages of cyathostomins. These molecular tools are now proving very useful in the investigation of the relative contributions that individual, common cyathostomin species make to the pathology and epidemiology of mixed helminth infections. At the more applied level, research is also in progress to develop an immunodiagnostic test that will allow numbers of mucosal larvae to be estimated. This test utilises antigen-specific IgG(T) serum antibody responses as markers of infection. As anthelmintic resistance will be the major constraint on the future control of the Cyathostominae, researchers are now actively investigating this area and studies aimed at elucidating the molecular mechanisms of drug resistance are described. Another parasite which has assumed a clinically important role in horses is the tapeworm, Anoplocephala perfoliata. This parasite is prevalent world-wide and has been shown to be a significant cause of equine colic. Because previous methods of estimating the infection intensity of tapeworm were inaccurate, recent research has been directed at developing an immunodiagnostic ELISA for these cestodes. Specific IgG(T) responses to antigens secreted by adult tapeworms have been shown to provide a reasonable indication of infection intensity. An ELISA based on these responses is now commercially available. The steps involved in the development of this ELISA are described here. In addition to these recent advances in research, this review also outlines the principle areas for future research into these important equin...

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Section: An Immunodiagnostic Assay For Equine Tapeworm Infectionmentioning

confidence: 99%

Recent developments in research into the Cyathostominae and Anoplocephala perfoliata

et al. 2004

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“…Her attan bireysel dışkı örnek-leri, ya rektal yoldan ya da taze yapılmış dışkının yerle temas etmeyen kısımlarından alınarak plastik poşetlere konmuş ve etiketlenmiş, ayrıca yaş, cinsiyet, ırk gibi bilgileri de kaydedilerek laboratuvara getirilmiştir. Dışkı örnekleri laboratuvarda doymuş şekerli su (d:1.30) kullanılarak sedimentasyon+santrifüj-flotasyon tekniği ile incelenmiştir (17).…”

Section: Materyal Ve Metotunclassified

“…Anoplocephalidae türlerinin biyolojileri gereği dışkıyla düzenli yumurta çıkışının olmaması, yumurta saptamaya dayalı dışkı muayene yöntemlerinin duyarlılığını azaltan önemli faktörlerden biridir. Bu handikapı ortadan kaldırabilmek için farklı yoğunlukta sıvı kullanan, farklı uygulama prosedürlerini içeren "dışkıda yumurta saptama yöntem-leri" üzerine yapılmış çalışmalar bulunmaktadır (1,17,21,24,28,32). Bu kapsamda örneğin doymuş şekerli su ile yapılan dışkı muayenelerinin daha iyi sonuç verdiği değişik araştırıcılar tarafından bildirilmiştir (20,24,32).…”

Section: Introductionunclassified

Dışkı bakısı sonuçlarına göre spor atlarında Anoplocephalidae enfeksiyonlarının prevalansı

GİRİŞGİN¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışma, sportif amaçlı (yarış, konkur, v.b.) at yetiştirilen odaklarda Anoplocephalidae enfeksiyonlarının prevalansını saptamak amacıyla yapılmıştır. Bu amaçla Bursa, Balıkesir, Kocaeli, Tekirdağ ve Eskişehir illerinde bulunan 18 odaktaki, farklı ırk, yaş ve cinsiyetten 460 atın taze dışkı örnekleri alınmıştır. Alınan örnekler sedimentasyon+santrifüj-flotasyon tekniği ile doymuş şekerli su (d:1.30) kullanılarak, Anoplocephalidae yumurtaları yönünden incelenmiştir. Atların 56'sında (%12.2) yumurta tespit edilmiş, 8 odakta (%44.4) ise en az bir atın enfekte olduğu saptanmıştır. Enfeksiyona odak bazında en düşük %4.3, en yüksek ise %70 olarak rastlanmıştır. Anoplocephalidae enfeksiyonunun, erkeklerde, 1-3 yaş arası atlarda ve safkan Arap ırkında daha yüksek oranda bulunduğu saptanmıştır.Anahtar sözcükler: Anoplocephala magna, Perfoliata, Mamillana, dışkı bakısı, prevalans, spor atı. Prevalence of Anoplocephalidae-infections according to fecal examination in sport horsesSummary: Aim of the present study was to determine the prevalance of Anoplocephalidae infections on enterprices where horses are kept for sport purposes (race, jumping, etc). A total of 460 horses of different age, gender and breed from 18 centres located in Bursa, Balıkesir, Kocaeli, Tekirdağ and Eskişehir were examined. Individual faecal samples were collected per rectum or freshly voided faeces. The samples were examined by means of sedimentation+centrifugation-flotation technique used saturated sugar solution (d=1.3) as medium. Anoplocephalidae-eggs were detected in 56 (12.2%) horses, whereas on 8 (44.4%) farms at least one horse was found to be infected. The lowest and the highest within farm prevalence was 4.3% and 70%, respectively. Males and Arabian purebred horses had significantly higher infection rates than females and the other breeds, respectively. Differences in prevalence in relation to age -animals between 1 and 3 years of age being more affected than older and younger ones-are seen.

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“…Hinzu kommt, dass ein hoher Anteil einer Bandwurm-Population steril sein kann (Schuster, 1991). Diese Faktoren in Summe bedingen die bei Untersuchungen zum Zestoden-Befall über-einstimmend festgestellten weit höheren Befallshäufigkeiten bei der Sektion im Vergleich zur Nachweishäufigkeit von Bandwurm-Entwicklungsstadien im Kot der Pferde (Lyons et al, 1983(Lyons et al, , 1984Beroza et al, 1986;Gauderon et al, 1988;Collobert et al, 1991;Proudman und Edwards, 1992;Ihler et al, 1995;Nilsson et al, 1995;Agneessens et al, 1998;Meana et al, 1998;Sanada et al, 1998;Beelitz und Gothe, 2001 (Brem und Wojtek, 1972;Ohms, 1973;Nebel, 1976;Keller und Fries, 1979;Bauer und Stoye, 1984;Epe et al, 1993Epe et al, , 1999. Auch im Vergleich zu der von Kölbl (1975) durch Untersuchung von 620 Pferdekotproben aus Bestän-den in Österreich in den Jahren 1969-1974 festgestellten Ausscheidungsextensität von 6,7% ist eine Verringerung der Nachweishäufigkeit festzustellen.…”

Section: Diskussionunclassified