Reliability of the Kirby-Bauer Disc Diffusion Method for Detecting Methicillin-Resistant Strains of Staphylococcus aureus

Drew, W. Lawrence; Barry, A. L.; O’Toole, Richard; Sherris, John C.

doi:10.1128/aem.24.2.240-247.1972

Cited by 130 publications

(59 citation statements)

References 13 publications

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“…The disk diffusion method is a reliable method of detection of methicillin resistance, provided a few precautions are taken. Resistance is readily detected at 350C or lower, but may be missed at 370C (54,156). Incubation temperatures of 30 and 350C give similar results for oxacillin, nafcillin, and methicillin (20, 54, 156).…”

Section: Disk Diffusionmentioning

confidence: 82%

Methicillin-resistant staphylococci.

Chambers

1988

Clinical Microbiology Reviews

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Section: Disk Diffusionmentioning

confidence: 82%

Methicillin-resistant staphylococci.

Chambers

1988

Clinical Microbiology Reviews

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“…All chemicals used were purchased from Sigma-Aldrich. The solvents used were purified by reported method [20]. Double distilled water has been used whereever necessary.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, Characterization and Antimicrobial Evaluation of Cobalt(III) Complexes of 4-(2-Substituted Phenylimino)-2-(4-Substituted Phenyl)-4H-chromen-3-ol

2019

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A series of eight Co(III) complexes [CoL1-8(H2O)2Cl] (I-1 to I-8) incorporating 4-(2-substituted phenylimino)-2-(4-substituted phenyl)-4H-chromen-3-ol, as a tridentate imino flavone ligands (L1 to L8, 2-sub. = NH2, SH, 4-sub. = OMe, OH, Cl, NMe2) have been synthesized, characterized and the geometry of the complexes were optimized by DFT. The chemical structure of synthesized imino flavone ligands and their complexes were characterized by elemental analysis, 1H NMR, 13C NMR, UV-visible, IR, ESI-mass spectral data, conductometric and magnetic measurements. The synthesized compounds have been screened for their in vitro antibacterial activities against bacteria Vibrio cholerae, Salmonella typhi, Staphylococcus aureus, Escherichia coli and antifungal activities against fungi Candida albicans and Aspergillus flavus by paper disc diffusion method. The complexes I-3, I-4, I-7 and I-8 showed good antimicrobial activities against pathogens.

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“…The antibacterial activity of the plant extract was performed by the well accepted Bauer-Kirby disc diffusion method against three Grampositive (Bacillus cereus, Bacillus subtilis and Staphylococcus aureus) and five gram-negative bacteria (Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Serratiama rcescens and Proteus mirrabillis) 15,16 .…”

Section: Antibacterial Activity By Disc Diffusion Methodsmentioning

confidence: 99%

Untitled

2018

IJPSR

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Oxidative stress is involved in the pathogenesis of various degenerative diseases like cancer, inflammation etc. Thus, the study of antioxidant activity of natural molecules has remained a fertile ground for the researchers. In continuation of that effort, the present study was designed to evaluate the antioxidant, cytotoxic, antibacterial and anti-inflammatory potential of chloroform extract of Stereospermum chelonoides leaves (SCLC). Preliminary phytochemical analysis followed by total phenol and total flavonoid determination assays were carried out. Antioxidant potential was evaluated using DPPH radical scavenging assay, total antioxidant capacity, ferric reducing antioxidant power and cupric reducing antioxidant assays. Brine shrimp lethality bioassay and MTT based cytotoxicity assay was also conducted. Antibacterial assay was carried out using disc diffusion method on both gram-positive and gram-negative bacteria. Croton oil and xylene induced ear oedema models were used to determine anti-inflammatory activity. Phytochemical screening ensured the presence of various secondary metabolites notably polyphenols (38.04 ± 0.77 mg/g, GAE) and flavonoids (130 ± 7.86, mg/g, QE) which explained its dose dependent antioxidant activities. Moreover, cupric ion reducing capacity of SCLC was as similar as standard ascorbic acid indicating strong metal chelating activity. In cytotoxicity assay, SCLC produced significant cell killing effect on hela cells in high doses. Significant antiinflammatory activity was observed in croton oil induced ear edema. Antioxidant activity of SCLC is suggestive of its ability to halt free radical generation process and its subsequent potential in the physiologic conditions adversely affected by radical generations.

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Reliability of the Kirby-Bauer Disc Diffusion Method for Detecting Methicillin-Resistant Strains of Staphylococcus aureus

Cited by 130 publications

References 13 publications

Methicillin-resistant staphylococci.

Methicillin-resistant staphylococci.

Synthesis, Characterization and Antimicrobial Evaluation of Cobalt(III) Complexes of 4-(2-Substituted Phenylimino)-2-(4-Substituted Phenyl)-4H-chromen-3-ol

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