Relocation of the replication terminus, terC, of Bacillus subtilis to a new chromosomal site

Iismaa, Tiina P.; Carrigan, C.M.; Wake, R.G.

doi:10.1016/0378-1119(88)90395-2

Cited by 5 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, mutants selected for deletions in a specific region on the chromosome have also been reported (for example, at the gnt locus [15] or the terC region [18]); however, these mutants were all induced under certain specific environmental stress or were selected. Thus, the extents and locations of the mutations are distinct from those found in this study.…”

Section: Discussionmentioning

confidence: 99%

“…To introduce the I-SceI site at the terC region, a neomycin resistance gene cassette (20) carrying the sequence neo(ISceI), (19) was inserted at the unique BglII site within the terC region cloned in pWS10 (terC) (18), resulting in pBMAP106SCB [terC::neo(I-SceI)]. pBMAP106SCB was used to transform B. subtilis 168 trpC2 and RM125, resulting in neomycin-resistant strains BEST2069 and BEST2071, respectively (Table 1).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Stability and asymmetric replication of the Bacillus subtilis 168 chromosome structure

Itaya

1993

J Bacteriol

View full text Add to dashboard Cite

Chromosomal DNAs from a number of strains derived from Bacillus subtilis 168 were digested with restriction endonucleases NotI or Sfil, and the locations of chromosomal alterations were compared with the recently constructed standard NotI-SfiI restriction map (M. Itaya and T. Tanaka, J. Mol. Biol. 220:631-648, 1991). In general, the chromosome structure of B. subtilis 168 was found to be stable, as expected from the genetic stability of this species. DNA alterations, typically deletions, are formed in three limited loci on the chromosome. One of these alterations was characterized as a spontaneous deletion formed between rrn operons, and another occurred as a result of prophage SPI3 excision. I found that oriC and terC are not located on precisely opposite sides of the chromosome. Replication in the counter clockwise direction was 196 kb longer than replication in the clockwise direction. The characteristic of length difference is not changed by deletion formation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%