Remediation of deterioration in microbial structure in continuous Pinellia ternata cropping soil by crop rotation

Abstract

Pinellia ternata is a traditional Chinese herb that suffers from continuous cropping (CC), which significantly decreases both yield and quality. The influence of CC on the microbiome in P. ternata rhizosphere and the effects of remediation on microbiota by rotational cropping (CR) were assessed by Illumina high-throughput sequencing technology. CC tends to decrease the α-diversities as a function of cultivation time, whereas CR tends to increase them. Differentially abundant analysis showed that microbial structure was important in maintaining the health status of P. ternata rhizosphere. Results suggested that CC soils were mainly enriched for Pseudomonas, Rhizobium, and Streptophyta operational taxonomic units (OTUs), while the CR soils were mainly enriched for Rhizobium, Pseudomonas, Flavobacterium, Sphingomonas, Rhizobacter, and Arthrobacter OTUs. On the basis of the community dissimilarities, we grouped all sample replicates into three post hoc clusters in which soils were defined as healthy, health-suppressed, and health-depressed soils. The three soil types represented different soil physicochemical properties. The activities of the microbiome features, including ammonia oxidizer, sulfate reducer, nitrite reducer, dehalogenation, xylan degrader, sulfide oxidizer, nitrogen fixation, atrazine metabolism, chitin degradation, degraded aromatic hydrocarbons, and chlorophenol degradation, were also considerably different among the three soils.

Nitrogen Fertilization in a Faba Bean–Wheat Intercropping System Can Alleviate the Autotoxic Effects in Faba Bean

“…As the essence of Chinese traditional agriculture, intercropping is an effective measure to alleviate the negative effects of continuous cropping by combining land use and land maintenance in agricultural production [ 41 ]. Reasonable intercropping can reduce the autotoxic effect and negative effect of continuous cropping [ 42 , 43 ]. Through the water culture and pot experiment, Zhao et al proved that American ginseng and P. frutescens intercropping could effectively overcome the autotoxic effect of the continuous cropping of American ginseng [ 16 ].…”

A green garlic (Allium sativum L.) based intercropping system reduces the strain of continuous monocropping in cucumber (Cucumis sativus L.) by adjusting the micro-ecological environment of soil

“…Long-term continuous cropping resulted in simplification and imbalance of the microbial structure, which is a critical factor contributing to continuous cropping obstacle (Fu et al, 2017). Many researches suggested that rotational cropping could relieve cropping obstacle by shifting the rhizosphere microbial community (Acosta-Martínez & Cotton, 2017; He et al, 2019). In this study, PCA analysis showed that more bacterial FAMEs biomarkers were accumulated in the intercropped soils (GB and GR), while more fungal FAMEs biomarkers were accumulated in the monoculture soils (CB and CR).…”

Advances in Soil Engineering: Sustainable Strategies for Rhizosphere and Bulk Soil Microbiome Enrichment

“…Sphingomonas and Rhizobium, may play critical functional and structural roles at different plant growth stages and in different crops [80]. Pinellia ternate, a traditional Chinese herb, also showed significant decreases for both yield and quality when exposed to continuous cropping, where soils enriched for Pseudomonas, Rhizobium, and Streptophyta were observed, while the crop rotation soils enriched for Pseudomonas, Rhizobium, Flavobacterium, Sphingomonas, Rhizobacter and Arthrobacter [47]. Alfalfa-rice rotation displayed variations in the relative abundance of Acidobacteria, Proteobacteria, Chloroflexi, Actinobacteria and Bacteroidetes [81].…”