Remifentanil-induced preconditioning has cross-talk with A1 and A2B adenosine receptors in ischemic-reperfused rat heart

Abstract

The purpose of this study was to determine whether there is a cross-talk between opioid receptors (OPRs) and adenosine receptors (ADRs) in remifentanil preconditioning (R-Pre) and, if so, to investigate the types of ADRs involved in the cross-talk. Isolated rat hearts received 30 min of regional ischemia followed by 2 hr of reperfusion. OPR and ADR antagonists were perfused from 10 min before R-Pre until the end of R-Pre. The heart rate, left ventricular developed pressure (LVDP),velocity of contraction (+dP/dtmax), and coronary flow (CF) were recorded. The area at risk and area of necrosis were measured. After reperfusion, the LVDP, +dP/dtmax,and CF showed a significant increase in the R-Pre group compared with the control group (no intervention before or after regional ischemia). These increases in the R-Pre group were blocked by naloxone, a nonspecific ADR antagonist, an A1 ADR antagonist, and an A2B ADR antagonist. The infarct size was reduced significantly in the R-Pre group compared with the control group. The infarct-reducing effect in the R-Pre group was blocked by naloxone, the nonspecific ADR antagonist, the A1 ADR antagonist, and the A2B ADR antagonist. The results of this study demonstrate that there is cross-talk between ADRs and OPRs in R-Pre and that A1 ADR and A2B ADR appear to be involved in the cross-talk.

Mechanisms involved in adenosine pharmacological preconditioning-induced cardioprotection

“…Various studies have documented the functional and physical interaction between adenosine and opioid receptors. Indeed, there is a cross-talk between these receptors in inducing cardioprotective effects [ 50 51 52 ]. It has been documented that adenosine may activate opioid receptors to induce cardioprotection during preconditioning [ 22 49 ].…”

“…Lee et al described that there is a cross-talk between opioid and adenosine receptors in remifentanil preconditioning-induced cardioprotection. The authors demonstrated that the protective effects of remifentanil were abolished by antagonists of opioid, A 1 and A 2B receptors suggesting the interrelationship between opioids and adenosine [ 50 ]. An earlier study also described the interaction between spinal mu-opioid receptors and adenosine in remote conditioning [ 51 ].…”

A Trial of Two Anesthetic Regimes for Minimally Invasive Mitral Valve Repair

“…18 Finally, sufentanil was used in both groups; opioids produce cardioprotection against ischemia-reperfusion injury in rodents in a manner potentially related to adenosine receptor cross-talk. 19 Future studies should address these important points in order to better inform the utility of different anesthetic preconditioning regimens in cardiac surgery.…”

Opioids in Remote Ischemic Preconditioning-Induced Cardioprotection

“…The potential cross talk between adenosine and opioid receptors in the heart has been shown by various groups of researchers. 83 -85 Peart and Gross revealed that adenosine and opioids elicit cardioprotective effects via cross talk between these respective receptors. The authors demonstrated that morphine-induced cardioprotective effects against sustained ischemia–reperfusion in rat model of myocardial infarction were abolished in the presence of selective A 1 receptor blocker, DPCPX.…”

“…This indicates that there might be an existence of potential cross talk between opioid and adenosine receptors during remifentanil preconditioning, and adenosine A 1 and A 2B receptors are likely involved in mediating the cardioprotective effects of opioids. 85…”