Remineralization of bleached enamel with novel nutraceutical agents: An in vitro study

Abstract

Background/Purpose: The eff ect of bleaching agents on dental hard tissues was uncertain. Moreover, reverting the side eff ects caused by bleaching using nutraceuticals as remineralizing agents remained uninvestigated. Hence, the aim of this study was to evaluate the eff ect of 22% carbamide peroxide (CP) on the mineral content of enamel and to evaluate the remineralization of bleached enamel after treatment with three diff erent remineralizing agents. Materials and Methods: A total of 30 extracted human maxillary incisors were selected, and their initial mineral content was analyzed using scanning electron microscopy with energy dispersive X-ray spectroscopy (EDX). All the specimens were bleached with 22% CP and their mineral content was reanalyzed. They were then randomly divided into three groups. Group A: Remineralization with casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), Group B: Remineralization with a 6.5% grape seed extract (GSE) solution prepared in phosphate buff er, and Group C: Remineralization with 6.5% wheat grass solution in phosphate buff er. Remineralization was carried out for 10 min, and EDX analysis was carried out again. Statistical analysis was done using oneway ANOVA and Student's t-test. Results: Bleaching with 22% CP signifi cantly decreased the mineral content of the enamel. All the three remineralizing agents signifi cantly increased the mineral content. Group A (CPP-ACP) showed the highest remineralization than Group B and C but was not statistically signifi cant. Conclusion: Within the limitations of the present study, it can be concluded that bleaching with CP decreases the mineral content of enamel and agents such as CPP-ACP, GSE, and wheat grass help in remineralizing the bleached surface.

Enamel Changes of Bleached Teeth Following Application of an Experimental Combination of Chitosan-Bioactive Glass

“…However, literature is controversial regarding the mineral content of tooth structure following bleaching. Some studies reported a signi cant reduction in both calcium and phosphorous contents after bleaching [9,24] while some others reported no signi cant change in this regard [1,26]. Junior et al [27] reported a reduction in calcium and an increase in phosphorous content after bleaching [27].…”

“…Many different remineralizing agents have been used to eliminate the adverse demineralizing effects of bleaching agents [8][9][10][11][12][13]. Bioactive glass (BG) is a biocompatible remineralizing agent that can induce cell signaling for tissue regeneration.…”

An In Vitro Evaluation of the Remineralizing Efficacy of Two concentrations of Silica Doped Nanohydroxyapatite on Bleached Enamel

“…All samples were decoronated using a double-faced diamond disc attached to a laboratory micromotor, tested for mineral content at three different thirds of the teeth using Energy dispersive X-Ray spectroscopy (14,15).…”