Remineralizing efficacy of fluoride in the presence of oral microcosm biofilms

Kang, Min-Kyung; Kim, Hee‐Eun

doi:10.1016/j.jdent.2021.103848

Cited by 10 publications

(4 citation statements)

References 20 publications

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“…Third, the substrates used to cultivate oral microcosm biofilms were the HA disks, which, while serving as an adequate model, differ from natural human enamel in several aspects. However, we used the same protocol to prepare the surfaces of the HA disks as that for human enamel, thus minimizing any potential effects on biofilm formation [43]. Future studies can replicate these experiments in human enamel substrates to more closely mimic in vivo conditions and assess CAP's treatment efficacy in a more clinically relevant context.…”

Section: Discussionmentioning

confidence: 99%

Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms

Kim

2024

Biomedicines

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As biofilms mature, biomass and extracellular polysaccharide (EPS) content increases, enhancing pathogenicity. Therefore, this study aimed to evaluate the antibacterial efficacy of cold atmospheric plasma (CAP) against oral microcosm biofilms and the influence of biofilm maturity on treatment. Oral microcosm biofilms were cultured on hydroxyapatite disks for 2 and 6 days. Based on the treatment and biofilm maturity, these were subsequently allocated into six groups (N = 19 each): Groups 1 and 2 were incubated with distilled water for 1 min; Groups 3 and 4 were treated with CAP for 2 min, and Groups 5 and 6 were treated with 0.12% chlorhexidine gluconate for 1 min. Groups 1, 3, and 5 represent 2-day biofilms, and Groups 2, 4, and 6 represent 6-day biofilms. Treatments were repeated daily for 5 days. Antibacterial efficacy was analyzed by measuring oral biofilms’ red fluorescence intensity (RatioR/G) and quantifying EPS content and bacterial viability. The RatioR/G was 1.089-fold and 1.104-fold higher in Groups 4 and 6 than in Groups 3 and 5 following antibacterial treatment, respectively (p < 0.001). EPS content increased by 1.71-fold in Group 6 than in Group 5 (p < 0.001). Bacterial survival rate was the lowest in Group 3 (p = 0.005). These findings underscore the relevance of CAP treatment in maintaining antibacterial efficacy regardless of the biofilm development stage, highlighting its potential utility in oral care.

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Section: Discussionmentioning

confidence: 99%

Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms

Kim

2024

Biomedicines

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“…Second, the biofilms were formed on HAP disks rather than on actual enamel, which could have influenced initial biofilm adherence. Nevertheless, the HAP disk surfaces were prepared similarly to the process of specimen preparation using actual enamel [25], thereby minimizing the potential impact of the HAP disk surface characteristics on biofilm formation. Third, the CAP treatment duration was set to 2 min based on the unique characteristics of the plasma application, accounting for the 1 mm diameter jet projection onto the 7 mm disk and the output pressure of 0.02 MPa.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Cold Atmospheric Plasma on Pathogenicity of Oral Microcosm Biofilms

Kim

2024

Applied Sciences

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This study aimed to compare the longitudinal efficacy between chlorhexidine gluconate (CHX; 0.12%) and cold atmospheric plasma (CAP) in reducing oral biofilm pathogenicity, utilizing a quantitative light-induced fluorescence-digital (QLF-D) camera. Oral microcosm biofilms were developed for 2 days on 57 hydroxyapatite disks. These biofilms were treated with distilled water for 1 min, CHX for 1 min, and CAP for 2 min over the course of 6 days. The red fluorescence intensities of the biofilms were measured using a QLF-D and expressed as pre- and post-treatment red/green ratios (RatioR/G). The bacterial viability (ratio of the green-stained area to the total stained area, RatioG/G+R) was calculated using live/dead bacterial staining; the total and aciduric bacterial counts were determined. A significant intergroup difference was found between RatioR/G changes according to the treatment period (p < 0.001). The RatioR/G observed within the CAP-treated group was significantly lower compared with the CHX-treated group at every interval of measurement (p < 0.001). The CAP-treated group also exhibited a lower RatioG/G+R and more weakened bacterial aggregation compared with the CHX-treated group (p < 0.05). In the group treated with CAP, the counts of both total and aciduric bacteria were substantially reduced compared with the DW group, with a statistically significant reduction (p < 0.001). Therefore, CAP may be more effective in minimizing oral microcosm biofilm pathogenicity than 0.12% CHX.

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“…This may influence the early attachment of the biofilm. However, we prepared the surfaces of HA disks using the same protocol for preparing specimens with human enamel [33]. Therefore, we minimized the potential effects of the HA disk surface on biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Effects of Non-Thermal Atmospheric Pressure Plasma on Oral Microcosm Biofilms

Cho

Kim

2023

IJERPH

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We comparatively evaluated the antibacterial effects of non-thermal atmospheric pressure plasma (NTAPP) on oral microcosm biofilms. Oral microcosm biofilms, which are derived from inoculation with human saliva, were cultured on 48 hydroxyapatite disks for 6 d. The prepared biofilms were divided into three different daily treatment groups: distilled water for 1 min, 0.12% chlorhexidine for 1 min, and NTAPP for 5 min. Using a quantitative light-induced fluorescence-digital camera, the red fluorescence intensity of the biofilms was measured as red/green ratios (RatioR/G) before and after treatment. Total and aciduric bacteria were counted as colony-forming units. Using live/dead bacterial staining, bacterial viability was calculated as the RatioG/G+R. RatioR/G was approximately 0.91-fold lower in group 3 than in group 2 on day 1 of treatment (p = 0.001), and approximately 0.94-fold lower on both days 2 and 3 (p < 0.001). The number of total bacteria was higher in group 3 than in group 2, but not significantly different. The number of aciduric bacteria was lowest in group 2 (p < 0.001). However, bacterial viability was lowest in group 3. Restricted bacterial aggregation was observed in group 3. These findings suggest that NTAPP may more effectively reduce the pathogenicity of oral microcosm biofilms than 0.12% chlorhexidine.

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Remineralizing efficacy of fluoride in the presence of oral microcosm biofilms

Cited by 10 publications

References 20 publications

Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms

Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms

Efficacy of Cold Atmospheric Plasma on Pathogenicity of Oral Microcosm Biofilms

Antimicrobial Effects of Non-Thermal Atmospheric Pressure Plasma on Oral Microcosm Biofilms

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