2022
DOI: 10.1021/acs.jafc.2c04454
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Remodeling Bacillus amyloliquefaciens Cell Wall Rigidity to Reduce Cell Lysis and Increase the Yield of Heterologous Proteins

Abstract: Bacillus amyloliquefaciens has great potential as a host for heterologous protein production, but its severe autolytic behavior has precluded its industrial application to date. Because d,l-endopeptidase activity-guided cell wall rigidity is considered essential for autolysis resistance, we investigated the effects of d,l-endopeptidase genes lytE, lytF, cwlO, and cwlS play on the growth, lysis, and morphology remodeling of B. amyloliquefaciens strain TCCC11018. Individual and combinatorial deletion of lytE, ly… Show more

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Cited by 4 publications
(2 citation statements)
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“…9 By deletion of the dlt operon (dlt ABCD), the production of nattokinase, α-amylase, and β-mannanase in Bacillus licheniformis were increased. 10 Tandem deletion of four autolysis genes in B. subtilis 168 also increased the expression of β-galactosidase and nattokinase. 11 Knockout of autolysis-related genes lytC and sigD effectively increased exogenous protein production and delayed autolysis by 10−15 days.…”
Section: ■ Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…9 By deletion of the dlt operon (dlt ABCD), the production of nattokinase, α-amylase, and β-mannanase in Bacillus licheniformis were increased. 10 Tandem deletion of four autolysis genes in B. subtilis 168 also increased the expression of β-galactosidase and nattokinase. 11 Knockout of autolysis-related genes lytC and sigD effectively increased exogenous protein production and delayed autolysis by 10−15 days.…”
Section: ■ Introductionmentioning
confidence: 99%
“…subtilis ATCC 6051 has been reported to increase the density of the bacterium and to improve the amylase activity by 1.6-fold . By deletion of the dlt operon ( dlt ABCD), the production of nattokinase, α-amylase, and β-mannanase in Bacillus licheniformis were increased . Tandem deletion of four autolysis genes in B.…”
Section: Introductionmentioning
confidence: 99%