Remodeling of neutrophil phospholipids with 15(S)-hydroxyeicosatetraenoic acid inhibits leukotriene B4-induced neutrophil migration across endothelium.

Interleukin-1.BETA. Stimulates Transendothelial Mobilization of Human Peripheral Blood Mononuclear Cells with a Potential to Differentiate into Osteoclasts in the Presence of Osteoblasts.

“…For studies of transmigration, HUVECs were grown to confluence in HUVEC medium on gelatincoated (1% Transwell polycarbonate filters containing 5 pm pores (Costar, Cambridge, MA), which were inserted into 24 well tissue culture plates (Costar, Cambridge, MA), as described previously [16]. SaOS-2 cells were grown to confluence on 24-well plates, unless otherwise specified.…”

Apoptotic human cells inhibit migration of granulocytes via release of lactoferrin

“…Therefore, at inflammatory sites, negative signals exist that prevent neutrophil recruitment, dampen neutrophil responsiveness, and counterbalance or terminate the inflammatory response. This antiinflammatory program is characterized by cessation of neutrophil infiltration, as arachidonic acid-derived prostaglandins and leukotrienes are switched to lipoxins, resolvins, and protectins (20,21). Resolution of inflammation is ultimately achieved through neutrophil apoptosis, and apoptotic neutrophils are subsequently phagocytosed by macrophages, a process that leads to the release not only of antiinflammatory cytokines but also of such antiinflammatory and proresolving lipid mediators as lipoxin A4, resolvin E1, and protectin D1 (22)(23)(24)(25).…”

Transfection of rat kidney with human 15-lipoxygenase suppresses inflammation and preserves function in experimental glomerulonephritis

“…The anti-inflammatory actions of 15-LO-derived eicosanoids during experimental glomerulonephritis demonstrated here are likely mediated through two major pathways: (a) specific antagonism of leukotriene synthesis, receptor interactions, and biologic effects (41)(42)(43)(44)(45)(46)(47)(48)(49), and (b) generalized inactivation of polymorphonuclear cell͞macrophage functions through remodeling of leukocyte membrane phospholipids (12,17,50).…”

“…This novel membrane priming event was shown to have an impact on signal transduction. Subsequently, our own group (13,14) and others (15)(16)(17)(18) have presented evidence supporting the specific capacity of 15(S)-HETE and LXA 4 to effectively paralyze polymorphonuclear cell and macrophage activation, chemotaxis, and transendothelial migration. Data from these studies suggest that substitution of 15(S)-HETE͞LXA 4 for esterified arachidonic acid in the diacylglycerol (DAG) component of phosphatidylinositol bisphosphate leads to remodeling of membrane topology, thereby thwarting recognition of inflammatory agonists and adhesion molecules by leukocyte surface receptors.…”