The rotational Doppler effect (RDE), as a counterpart of the conventional well-known linear Doppler effect in the rotating frame, has attracted increasing attention in recent years for rotating object detection. However, the effect of the beam size on the RDE is still an open question. In this article, we investigated the influence of the size of the probe light; i.e., the size of the ring-shaped orbital angular momentum (OAM)-carrying optical vortex (OV), on the RDE. Both the light coaxial and noncoaxial incident conditions were considered in our work. We analyzed the mechanism of the influence on the RDE under the light coaxial, lateral misalignment, and oblique incidence conditions based on the small-scatterer model. A proof-of-concept experiment was performed to verify the theoretical predictions. It was shown that both the signal-to-noise ratio and the frequency spectrum width were related to the OV size. The larger the beam size, the stronger the RDE signal observed in the practical detection. Especially in the lateral misalignment condition, the large OV size effectively reduced the signal spreading and enhanced the signal strength. These findings may be useful for practical application of the optical RDE in remote sensing and metrology.