Laser cutting is a promising technology for the singulation of conventional and advanced electrodes for lithium-ion batteries. Even though the continuous development of laser sources, beam guiding, and handling systems enable industrial relevant high cycle times, there are still uncertainties regarding the influence of, for this process, typical cutting edge characteristics on the electrochemical performance. To investigate this issue, conventional anodes and cathodes were cut by a pulsed fiber laser with a central emission wavelength of 1059–1065 nm and a pulse duration of 240 ns. Based on investigations considering the pulse repetition frequency, cutting speed, and line energy, a cell setup of anodes and cathodes with different cutting edge characteristics were selected. The experiments on 9 Ah pouch cells demonstrated that the cutting edge of the cathode had a greater impact on the electrochemical performance than the cutting edge of the anode. Furthermore, the results pointed out that on the cathode side, the contamination through metal spatters, generated by the laser current collector interaction, had the largest impact on the electrochemical performance.