Remote ischemic preconditioning does not influence lectin pathway protein levels in head and neck cancer patients undergoing surgery

Abstract

BackgroundCancer patients who undergo tumor removal, and reconstructive surgery by transfer of a free tissue flap, are at high risk of surgical site infection and ischemia-reperfusion injury. Complement activation through the lectin pathway (LP) may contribute to ischemia-reperfusion injury. Remote ischemic preconditioning (RIPC) is a recent experimental treatment targeting ischemia-reperfusion injury. The study aims were to investigate LP protein plasma levels in head and neck cancer patients compared with healthy individuals, to explore whether RIPC affects LP protein levels in head and neck cancer surgery, and finally to examine the association between postoperative LP protein levels and the risk of surgical site infection. MethodsHead and neck cancer patients (n = 60) undergoing tumor resection and reconstructive surgery were randomized 1:1 to RIPC or sham intervention administered intraoperatively. Blood samples were obtained preoperatively, 6 hours after RIPC/sham, and on the first postoperative day. LP protein plasma levels were measured utilizing time-resolved immunofluorometric assays. ResultsH-ficolin and M-ficolin levels were significantly increased in cancer patients compared with healthy individuals (both P � 0.02). Conversely, mannan-binding lectin (MBL)-associated serine protease (MASP)-1, MASP-3, collectin liver-1 (CL-L1), and MBL-associated protein of 44 kilodalton (MAp44) levels were decreased in cancer patients compared with healthy individuals (all P � 0.04). A significant reduction in all LP protein levels was observed after surgery (all P < 0.001); however, RIPC did not affect LP protein levels. No difference was demonstrated in postoperative LP protein levels between patients who developed surgical site infection and patients who did not (all P > 0.13).

COVID-19: Oxidative Preconditioning as a Potential Therapeutic Approach

“…In randomized controlled trials, in patients undergoing surgical brain tumors, remote ischemic preconditioning enhanced cerebral perfusion and improved the surgery outcome by reducing the incidence of postoperative brain ischemia in these patients. − Despite the very high safety profile of preconditioning (i.e., patients with severe carotid artery stenosis), it should be carefully monitored when applied to COVID-19 patients.…”

Loss of ficolin-3 expression is associated with poor prognosis in patients with hepatocellular carcinoma

“…High expressions of FCN3 have been demonstrated in the liver and lungs, with the liver assumed to account for circulating levels [9]. Previously, a lower expression of FCN3 has been found and proved either in tissue or serum from numerous cancer patients such as ovarian cancer, multiple myeloma, lymphoma, acute myeloid leukemia, head and neck cancer, and lung cancer [8,[10][11][12][13][14][15]. However, few studies have investigated differences in the expression of FCN3 between patients with HCC with/without hepatitis viral infection and normal tissue, and the possible role of FCN3 in O-GlcNAcylation recognition in bioinformatic, cell line, RNA, and serum experiments [16][17][18].…”