Remote Ischemic Preconditioning on Neovascularization and Follicle Viability on Ovary Autotransplantation in Rats

Damous, Luciana Lamarão; Silva, S.M.; Simões, Ricardo Santos; Morello, Ricardo José; Carbonel, A.P.F.; Simões, Manuel; Montero, Edna Frasson de Souza

doi:10.1016/j.transproceed.2008.02.065

Cited by 15 publications

(13 citation statements)

References 31 publications

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“…In the animals submitted to R-IPC, this was performed by clamping the common iliac artery, immediately bellow the aorta bifurcation, with two vascular micro clamps for a 15 minute ischemia period, followed by a 15 minute reperfusion 9 , before the other procedures above described were performed (ooforectomy and transplant). In the group without R-IPC, the animals underwent a thirty minute observation period which corresponded to R-IPC time in order to standardize the operatory timing.…”

Section: Methodsmentioning

confidence: 99%

“…In one of our previous studies it was reported that R-IPC for a 15 minute period caused a better early graft revascularization via VEGF (Vascular endothelial growth factor) as well as a better preservation of ovarian follicles 9 . Thus, the object of the present study is to evaluate the R-IPC impact on the quality of the ovarian graft by means of vaginal smear of transplanted female rats.…”

Section: Resumo Objetivomentioning

confidence: 97%

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Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning

et al. 2009

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Purpose:To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats. Methods: Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles. Results: R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05). Conclusion: R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones.

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Section: Methodsmentioning

confidence: 99%

Section: Resumo Objetivomentioning

confidence: 97%

Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning

et al. 2009

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“…Considering that the IPC protects the gut in hypothermic condition improving post-transplantation 7 ; the mechanism involves R-IPC different paths of action in relation to IPC, and that in a previous study in our lab, showed increased IPC-R cell proliferation and neovascularization of the ovarian graft 8 ; it was decided to verify the role of intestinal transplantation IPC-R and its impact on intestinal development and in the process of acute allogeneic rejection.…”

Section: Introductionmentioning

confidence: 99%

Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice

et al. 2016

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PURPOSE:To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model. METHODS:Two groups were constituted: The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups: Tx, IPC-R, Fk, IPC-Fk, in both strains. Intestinal grafts were stained with hematoxylin and eosin and immunohistochemically. RESULTS:The graft development evaluation in ISO group showed that IPC-R reduced the development compared with ISO-Tx and Fk: 9.0±0.6). The graft rejection was lower in groups treated with IPC-R (-18%), Fk (-68%) or both (-61%) compared with ALO-Tx. CONCLUSION:Remote ischemic preconditioning showed benefic effect even associate with Tacrolimus on the development and acute rejection of the fetal small bowel graft in the Isogenic and Allogenic transplants.

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“…In a study of xenotransplantation, Lan et al [11] demonstrated that the 6-hour fetal ovary culture without hypoxia prior to transplantation improved graft viability and increased the proportion of primordial and preantral ovarian follicles. Previous studies of ours showed that ischemic preconditioning of the ovary improves graft quality [12,13]. This motivated us to research other preconditioning forms to determine whether they could also increase the viability and longevity of grafts.…”

Section: Introductionmentioning

confidence: 99%

Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function

Damous

Nakamuta

Soares-Jr

et al. 2014

Journal of Ovarian Research

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BackgroundCryopreservation of the ovarian tissue has shown promising results. However, there remain controversial issues such as the short half-life of grafts. In this aspect, there are some evidences that preconditioning the ovarian tissue before transplantation is beneficial.ObjectiveTo determine the effect of hypoxic preconditioning in vitro on ovarian tissue prior to transplantation.MethodsEighteen female adult Wistar rats, were sorted into three experimental groups. Ovaries were maintained in DMEM low glucose serum free at 37°C with 5% CO2, at atmospheric oxigen concentration (normoxia) or 1% O2 (hypoxia) for 16 hours. Oxigen concentration was determined by injection of nitrogen in the incubator. Animals submitted to ovarian transplantation immediately after oophorectomy were the Control Group (C). After this, the ovaries were implanted in the retroperitoneum with nonabsorbable suture and animals evaluated for thirty days after transplantation. Beginning on postoperative (PO) day 11, a daily collection of vaginal smear was carried out. Analyses comprised morphological, morphometric (counting ovarian follicles and corpora lutea) and immunohistochemistry for cleaved caspase-3 (apoptosis).ResultsIn normoxia and control groups all animals recovered their estrous cycles, while in the hypoxia group, two animals did not ovulate but, among those which did, resumption took longer than in the other groups (p < 0.05). The number of ovarian follicles and corpora lutea decreased significantly in the hypoxia group when compared to the other two groups (p < 0.001) and apoptosis was increased in the few ovarian follicles which remained viable (p < 0.001).ConclusionThe hypoxic preconditioning in vitro was not beneficial to the graft and worsened their viability, compromising its functionality or delaying the return of this.

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Remote Ischemic Preconditioning on Neovascularization and Follicle Viability on Ovary Autotransplantation in Rats

Cited by 15 publications

References 31 publications

Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning

Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning

Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice

Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function

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