Removal of Biofilms from Tracheoesophageal Speech Valves Using a Novel Marine Microbial Deoxyribonuclease

Abstract

The nontoxic deoxyribonuclease NucB was effective in releasing more microorganisms from biofilms on tracheoesophageal speech valves. This reflects its potential ability to break up and disperse these biofilms. Future studies will aim to develop NucB as a novel agent to prolong the life span of tracheoesophageal speech valves, thus reducing health care costs.

Evolving Marine Biomimetics for Regenerative Dentistry

“…In a special finding during an investigation into shifting films on seaweeds, the bacterial biofilms formed by a ubiquitous Bacillus licheniformis released an enzyme that allowed bacterial dispersal from the embedding medium of the biofilm. The NucB enzyme involved in this process is being developed to disrupt biofilms on the tooth surface after the successful disruption of the biofilms that cause sinusitis [119,120]. However, it remains to be seen whether this enzyme is generic and can effectively dislocate the biofilm structures secreted by other species.…”

Use of physiological information and process optimisation enhances production of extracellular nuclease by a marine strain of Bacillus licheniformis

“…Recently we reported a secreted bacterial nuclease, NucB, as an alternative novel biofilm dispersing enzyme. NucB was hypothesised to be released as an important enzyme for biofilm degradation and its mechanism of action on dispersing preformed biofilms is very effective (Nijland et al, 2010;Shakir et al, 2012). This could potentially be extended to breakup the ubiquitously present eDNA, leading to loosening of the EPS matrix and complete removal of majority biofilms.…”

Lipopeptide biosurfactants fromPaenibacillus polymyxainhibit single and mixed species biofilms

“…The marine environment and especially biofilm bacteria themselves have proven to be a rich source of antibiofilm compounds (Burgess et al 2003;Ortega-Morales et al 2008). Some of these compounds are involved in the detachment of biofilms, for example lipopeptides (Janek et al 2012), surfactins (Mireles et al 2001), glycolipids (Dusane et al 2011), whilst others can destabilise the biofilm matrix (Shakir et al 2012).…”

“…Surfaces with different physical properties such as glass may promote a different biofilm composition from those examined in this investigation. Shakir et al (2012) have also suggested that techniques such as SEM may not be appropriate for the quantification of biofilms with any significant depth.…”

“…In recent years, there has been an increase in solutions to biofilm inhibition such as modifications in the design of the substratum material (Hook et al 2012) and the disruption of the biofilm matrix (Shakir et al 2012). However, one of the most promising avenues has been the use of biosurfactants such as those described in this manuscript (Rivardo et al 2009;Banat et al 2010;Dusane et al 2011;Janek et al 2012).…”