Removal of both Ycf48 and Psb27 in Synechocystis sp. PCC 6803 disrupts Photosystem II assembly and alters QA− oxidation in the mature complex

Jackson, Simon A.; Hervey, John R. D.; Dale, Asher J.; Eaton‐Rye, Julian J.

doi:10.1016/j.febslet.2014.08.024

Cited by 31 publications

(43 citation statements)

References 45 publications

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“…Our results also point to a functional connection, since the expression profile for psb27 closely resembled that of slr0171 ( ycf37 ) ( Fig. S10 ; cluster J), for which the corresponding protein contributes to assembly and stabilization of PSI complexes 45 46 . Likewise, the co-transcription of psbN with genes encoding core subunits of both PSII and PSI ( Fig.…”

Section: Discussionsupporting

confidence: 59%

The Transcriptional Landscape of the Photosynthetic Model Cyanobacterium Synechocystis sp. PCC6803

Hernández‐Prieto

Semeniuk

Giner‐Lamia

et al. 2016

Sci Rep

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Cyanobacteria exhibit a great capacity to adapt to different environmental conditions through changes in gene expression. Although this plasticity has been extensively studied in the model cyanobacterium Synechocystis sp. PCC 6803, a detailed analysis of the coordinated transcriptional adaption across varying conditions is lacking. Here, we report a meta-analysis of 756 individual microarray measurements conducted in 37 independent studies-the most comprehensive study of the Synechocystis transcriptome to date. Using stringent statistical evaluation, we characterized the coordinated adaptation of Synechocystis’ gene expression on systems level. Evaluation of the data revealed that the photosynthetic apparatus is subjected to greater changes in expression than other cellular components. Nevertheless, network analyses indicated a significant degree of transcriptional coordination of photosynthesis and various metabolic processes, and revealed the tight co-regulation of components of photosystems I, II and phycobilisomes. Detailed inspection of the integrated data led to the discovery a variety of regulatory patterns and novel putative photosynthetic genes. Intriguingly, global clustering analyses suggested contrasting transcriptional response of metabolic and regulatory genes stress to conditions. The integrated Synechocystis transcriptome can be accessed and interactively analyzed via the CyanoEXpress website (http://cyanoexpress.sysbiolab.eu).

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Section: Discussionsupporting

confidence: 59%

The Transcriptional Landscape of the Photosynthetic Model Cyanobacterium Synechocystis sp. PCC6803

Hernández‐Prieto

Semeniuk

Giner‐Lamia

et al. 2016

Sci Rep

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“…Those results clearly demonstrated that enhanced ROS production can be a direct consequence of an impaired PSII repair cycle with implications for virusinduced suppression of ftsH expression in our study. Other research conducted on HCF136 knockouts in Synechocystis demonstrated an enhanced susceptibility to photodamage, which is thought to arise from compromised PSII reaction centers produced by an impaired assembly pathway (Jackson et al 2014). PSII photoinhibition and repair is also thought to be one strategy to protect PSI from irreversible damage (Tikkanen et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression is tied to photochemical efficiency reduction in virally infected Emiliania huxleyi

Gilg¹,

Archer²,

Floge³

et al. 2016

Mar. Ecol. Prog. Ser.

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“…This data collection procedure enabled normalization of the spectrum at 580 nm to the fluorescein peak observable only in the excitation spectrum at 440 nm. Variable chlorophyll a fluorescence relaxation and induction measurements were performed at room temperature as described by Jackson et al (26). For the derivation of the phycobilisome-to-PSII energy transfer capacity, we took F(J) to be the fluorescence maximum occurring after approximately 2.5 ms of actinic illumination; the amplitude of this point for the blue measuring flash data correlated with the variable fluorescence (F V ) determined from the single-turnover fluorescence relaxation experiments.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of Photosynthesis in a Glycogen-Deficient glgC Mutant of Synechococcus sp. Strain PCC 7002

Jackson

Eaton‐Rye

Bryant

et al. 2015

Appl Environ Microbiol

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c Cyanobacterial glycogen-deficient mutants display impaired degradation of light-harvesting phycobilisomes under nitrogenlimiting growth conditions and secrete a suite of organic acids as a putative reductant-spilling mechanism. This genetic background, therefore, represents an important platform to better understand the complex relationships between light harvesting, photosynthetic electron transport, carbon fixation, and carbon/nitrogen metabolisms. In this study, we conducted a comprehensive analysis of the dynamics of photosynthesis as a function of reductant sink manipulation in a glycogen-deficient glgC mutant of Synechococcus sp. strain PCC 7002. The glgC mutant showed increased susceptibility to photoinhibition during the initial phase of nitrogen deprivation. However, after extended periods of nitrogen deprivation, glgC mutant cells maintained higher levels of photosynthetic activity than the wild type, supporting continuous organic acid secretion in the absence of biomass accumulation. In contrast to the wild type, the glgC mutant maintained efficient energy transfer from phycobilisomes to photosystem II (PSII) reaction centers, had an elevated PSII/PSI ratio as a result of reduced PSII degradation, and retained a nitrogen-repletetype ultrastructure, including an extensive thylakoid membrane network, after prolonged nitrogen deprivation. Together, these results suggest that multiple global signals for nitrogen deprivation are not activated in the glgC mutant, allowing the maintenance of active photosynthetic complexes under conditions where photosynthesis would normally be abolished. Nitrogen is an essential macronutrient required for the synthesis of pigments, proteins, and nucleic acids in cyanobacteria. When nitrogen availability is limiting for cell growth and proliferation, a defined sequence of stress responses is deployed in most cyanobacteria. The immediate response is to increase the expression of genes associated with nitrogen uptake and assimilation (1-4). This is followed within hours by the cessation of growth and the mobilization of internal nitrogen stores through the degradation of the light-harvesting phycobilisomes, which contain nitrogen-rich phycobiliproteins and phycocyanin (5-7). Phycobilisome degradation also serves to reduce the light-harvesting cross section of the cell, thus avoiding excessive photon absorption, which can result in photooxidative damage in the absence of downstream metabolic oxidation reactions. The simultaneous activation of glycogen biosynthesis, degradation of thylakoid membranes, and reduction in the expression of genes associated with photosynthesis, carbon fixation, and de novo protein synthesis define the short-term acclimation events in response to nitrogen limitation (1-4). The longer-term acclimation in response to nitrogen limitation involves a decrease in metabolic activity to a minimum level that supports cell viability using energy derived from the catabolism of glycogen and cyclic electron transfer around photosystem I (PSI) (2, 3).The reductio...

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Removal of both Ycf48 and Psb27 in Synechocystis sp. PCC 6803 disrupts Photosystem II assembly and alters Q_A⁻ oxidation in the mature complex

Cited by 31 publications

References 45 publications

The Transcriptional Landscape of the Photosynthetic Model Cyanobacterium Synechocystis sp. PCC6803

The Transcriptional Landscape of the Photosynthetic Model Cyanobacterium Synechocystis sp. PCC6803

Differential gene expression is tied to photochemical efficiency reduction in virally infected Emiliania huxleyi

Dynamics of Photosynthesis in a Glycogen-Deficient glgC Mutant of Synechococcus sp. Strain PCC 7002

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