Removal of cartilage rings of the graft and omentopexy for extended tracheal autotransplantation

Failure of airway healing in an ovine autotransplantation model that includes basic fibroblast growth factor

“…Single-stage and multistage procedures compete with one another in this case. 14,15 Successful procedures involving muscular wrapping 3,16 and the greater omentum 14,17 have been described. Cryopreservation, 12,13,[18][19][20] detergents, 21 and immunosuppression 22 compete as methods of reducing immunogenicity.…”

“…Whether the almost obligatory pulmonary contamination of the sheep plays a role in this remains unclear. The reason that certain study groups required extensive intervention to achieve the same purpose 3,7,14,16,17 also remains unclear.…”

Current Solutions for Long-Segment Tracheal Reconstruction

“…This was further modified by Murai and colleagues. 19) when they removed the cartilage rings in order to increase contact to improve blood supply and re-epithelialization. In an attempt to overcome rejection, cryopreservation of the allografts was used and resulted in success even without immunosuppression.…”

Seeding of Recipient‐Originated Epithelial Cells Attenuates Epithelial to Mesenchymal Transition in Rat Tracheal Allotransplantation

“…Each donor tracheal transplant was isometrically divided into 3 segments of about 4 cartilage rings to allow for improved blood supply after implantation. 20 Then the 3 segments were successively implanted into 3 different dorsal subcutaneous sites of each recipient. Tracheal epithelial cells were prepared from untreated Wistar rats (n = 30) 4 days after transplantation and then seeded into the donor tracheas in the seeded allogeneic group after 2 days of isolating.…”