Removal of copper improves the lipid content in Nannochloropsis oculata culture

Aguilar-Ruiz, Rocio Janeth; Martinez‐Macias, María del Rosario; Sánchez‐Machado, Dalia I.; López‐Cervantes, Jaime; Dévora-Isiordia, Germán Eduardo; Nateras-Ramírez, Omar

doi:10.1007/s11356-020-10283-4

Cited by 12 publications

(2 citation statements)

References 54 publications

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“…Stress triggers many metabolic responses in microalgae, such as the degradation of intracellular compounds such as proteins, chlorophyll, and DNA, and the accumulation of energy‐rich compounds like lipids and carbohydrates. Aguilar‐Ruiz et al (2020) indicated that stress conditions as emerging pollutant metals like copper present in the culture medium favor the production of lipids.…”

Section: Resultsmentioning

confidence: 99%

Influence of different reactor types on Nannochloropsis oculata microalgae culture for lipids and fatty acid production

Martinez‐Macias

Aguilar-Ruiz

Nateras-Ramírez

et al. 2021

J Americ Oil Chem Soc

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Greenhouse gases emitted into the atmosphere by burning of fossil fuels cause global warming. One option is obtaining biodiesel. Nannochloropsis oculata was cultured under different light intensities and reactors at 25°C for 21 days with f/2 medium to assess their effects on cell density, lipid, and fatty acids (FAs). N. oculata improved cell density on fed‐batch glass tubular reactor (7 L) at 200 μmol E m−2 s−1, yielding 3.5 × 108 cells ml−1, followed by fed‐batch Erlenmeyer flask (1 L) at 650 μmol E m−2 s−1 with 1.7 × 108 cells ml−1. The highest total lipid contents (% g lipid × g dry biomass−1) were 44.4 ± 0.8% for the reactor (1 L) at 650 μmol E m−2 s−1 and 35.2 ± 0.2% for the tubular reactor (7 L) at 200 μmol E m−2 s−1, until twice as high compared with the control culture (Erlenmeyer flask 1 L, 80 μmol E m−2 s−1) with 21.2 ± 1%. Comparing the total lipid content at 200 μmol E m−2 s−1, tubular reactor (7 L) and reactor 1 L achieved 35.2 ± 0.2% and 28.3 ± 1%, respectively, indicating the effect of shape reactor. The FAs were affected by high light intensity, decreasing SFAs to 2.5%, and increased monounsaturated fatty acids + polyunsaturated fatty acids to 2.5%. PUFAs (20:5n‐3) and (20:4n‐3) were affected by reactor shape, decreasing by half in the tubular reactor. In the best culture, fed‐batch tubular reactor (7 L) at 200 μmol E m−2 s−1 contains major FAs (16:0; 38.06 ± 0.16%), (16:1n‐7; 30.74 ± 0.58%), and (18:1n‐9; 17.15 ± 0.91%).

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Section: Resultsmentioning

confidence: 99%

Influence of different reactor types on Nannochloropsis oculata microalgae culture for lipids and fatty acid production

Martinez‐Macias

Aguilar-Ruiz

Nateras-Ramírez

et al. 2021

J Americ Oil Chem Soc

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“…Where Y is the number of cells (C mL -1 ) and X denotes the OD at 680 nm. The specific growth rate (SGR) (μ, in day -1 ) was calculated in the exponential phase using eq (2), as described by Aguilar-Ruiz et al (2020).…”

Section: Growth Inhibition Cells Viability and Size Analysismentioning

confidence: 99%

Mechanisms of Lead (II) Phycoremediation Using Scenedesmus Obliquus: Cell Physicochemical Properties, Biochemical Composition and Metabolomic Profiling

Danouche

Aasfar

et al. 2021

Preprint

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This study highlights the mechanisms involved in Pb(II)-phycoremediation using the Pb(II) tolerant strain of Scenedesmus obliquus. Firstly, monitoring of cell growth kinetics in control and Pb(II)-doped medium revealed a significant growth inhibition, while the analyses through flow cytometry and Zetasizer revealed no difference in cell viability and size. Residual weights of control and Pb(II)-loaded cells assessed by thermogravimetric analysis, were 31.34% and 57.8%, respectively, indicating the uptake of Pb(II) into S. obliquus cells. Next, the use of chemical extraction to distinguish between the intracellular and extracellular uptake indicated the involvement of both biosorption (85.5%) and bioaccumulation (14.5%) mechanisms. Biosorption interaction of the cell wall and Pb(II) was confirmed by SEM, EDX, FTIR, zeta potential, zero-charge pH, and contact angle analyses. Besides, the biochemical characterization of control and Pb(II)-loaded cells revealed that the bioaccumulation of Pb(II) induces significant increases in the carotenoids and lipids content and decreases in the proportions of chlorophyll, carbohydrates, and proteins. Finally, the metabolomic analysis indicated an increase in the relative abundance of fatty acid methyl esters, alkanes, aromatic compounds, and sterols. However, the alkenes and monounsaturated fatty acids decreased. Such metabolic adjustment may represent an adaptive strategy, that preventing high Pb(II)-bioaccumulation in cellular compartments.

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