Removal of domain D2 or D3 of the human urokinase receptor does not affect ligand affinity

Stimulation of urokinase-type plasminogen activator receptor expression by PMA requires JNK1-dependent and -independent signaling modules

“…Urokinase can bind speci®cally and with high a nity (K D 90.5 nM) to a 45 ± 60 kDa heavily glycosylated cell surface receptor (u-PAR) (Stoppelli et al, 1986;Vassalli et al, 1985). The receptor is comprised of three similar repeats approximately 90 residues each Riittinen et al, 1996). The aminoterminal domain binds the plasminogen activator with the carboxyterminus domain serving to anchor the binding protein to the cell surface via a glycosylphosphatidylinositol chain Riittinen et al, 1996).…”

“…The receptor is comprised of three similar repeats approximately 90 residues each Riittinen et al, 1996). The aminoterminal domain binds the plasminogen activator with the carboxyterminus domain serving to anchor the binding protein to the cell surface via a glycosylphosphatidylinositol chain Riittinen et al, 1996). The u-PAR gene is 7 exons long and is located on chromosome 19q13 (Vagnarelli et al, 1992;Casey et al, 1994).…”

Elevated urokinase-type plasminogen activator receptor expression in a colon cancer cell line is due to a constitutively activated extracellular signal-regulated kinase-1-dependent signaling cascade

“…Several studies have shown that urokinase can bind to a 55 ± 60 kDa heavily glycosylated, disul®de linked, cell surface receptor (u-PAR) speci®cally and with high a nity (K D^0 .5 nM) (Stoppelli et al, 1986;Vassalli et al, 1985). This receptor is comprised of three similar repeats approximately 90 residues each Riittinen et al, 1996) the ®rst of which is required for the binding of urokinase and the last which is anchored to the cell membrane via a glycosyl-phosphatidylinositol chain . The 7 exon human u-PAR gene has been located to chromosome 19q13 (Vagnarelli et al, 1992;Casey et al, 1994) and is transcribed into a 1.4 kb mRNA or an alternatively spliced variant which lacks the carboxy-terminal membrane attachment peptide sequence (Roldan et al, 1990;Pyke et al, 1993).…”

Targeted disruption of the K-Ras oncogene in an invasive colon cancer cell line down-regulates urokinase receptor expression and plasminogen-dependent proteolysis

“…Urokinase can bind specifically and with high affinity (K D Ӎ0.5 nM) to a 45-60 kDa, heavily glycosylated, cell surface receptor (u-PAR) (Vassalli et al, 1985;Stoppelli et al, 1986) comprised of three similar repeats of approximately 90 residues each Riittinen et al, 1996). The aminoterminal domain binds the plasminogen activator with the carboxyterminus domain anchoring the binding protein to the cell surface via a glycosyl-phosphatidylinositol chain Riittinen et al, 1996).…”

“…Urokinase can bind specifically and with high affinity (K D Ӎ0.5 nM) to a 45-60 kDa, heavily glycosylated, cell surface receptor (u-PAR) (Vassalli et al, 1985;Stoppelli et al, 1986) comprised of three similar repeats of approximately 90 residues each Riittinen et al, 1996). The aminoterminal domain binds the plasminogen activator with the carboxyterminus domain anchoring the binding protein to the cell surface via a glycosyl-phosphatidylinositol chain Riittinen et al, 1996). The 7 exon u-PAR gene is located on chromosome 19q13 (Vagnarelli et al, 1992;Casey et al, 1994) and transcription of the gene yields a 1.4-kb mRNA or an alternatively spliced variant lacking the membrane attachment peptide sequence (Roldan et al, 1990;Pyke et al, 1993).…”