Removal of Human Leukemic Cells from Peripheral Blood Mononuclear Cells by Cell Recognition Chromatography with Size Matched Particle Imprints

Chester, Rosie; Das, Anupam A. K.; Medlock, Jevan; Nees, Dieter; Allsup, David; Madden, Leigh A.; Paunov, Vesselin N.

doi:10.1021/acsabm.9b00770

Cited by 2 publications

(2 citation statements)

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“…[45] The most important step of the process, the immobilization of the antigen of interest, can be accomplished by either direct adsorption to the assay plate, or indirectly via a capture antibody that has been pre-attached to the plate beforehand. [57,58] The sandwich ELISA, or indirect detection method, uses a labelled secondary antibody for detection and is the most popular format for ELISA. This type of capture assay is called a "sandwich" assay because the analyte to be measured is bound between two antibodies-the capture primary antibody and the detection antibody.…”

Section: Sandwich Elisa-based Bioimprint Detection Methodsmentioning

confidence: 99%

Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

2020

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We have developed and tested a novel ELISA-like approach for bacterial detection based upon selective adhesion of targeted bacteria to microwells with prefabricated bacterial bioimprints. Bioimprints were produced from three bacterial species; Escherichia coli (Gram-negative), Rhodococcus rhodochrous (Gram-positive) and Sarcina aurantiaca (Gram-negative), by using molding with curable silicone from dense layers of bacterial cells deposited on a glass substrate. We demonstrated that the surface functionalized whole cell bioimprints were able to selectively recognize and bind their own bacterial cell type. In order to detect target bacteria that are bound to the matching bioimprint, we also developed silica nanoparticles dual-functionalized with (3-glycidyloxypropyl)trimethoxysilane (GLYMO) coupled with 4-hydroxyphenylboronic acid (4-HPBA), SiO 2 NPs/GLYMO/4-HPBA, which were further conjugated with horseradish peroxidase (HRP). Bacterial detection was demonstrated to work in the established ELISA-like protocol using the colorimetric reaction of the conjugated HRP with 3,3′,5,5′tetramethylbenzidine (TMB). The bioimprints were used instead of capture antibodies and HRP-coated dual functionalized silica nanoparticles instead of a secondary antibody with TMB as the enzyme-converted reagent, producing a colored byproduct. This bacterial bioimprint-based detection method does not rely on any antibodies, uses stable and inexpensive reagents, and could potentially find application for rapid diagnostics of bacterial pathogens in clinical samples at the point of care. K E Y W O R D S bacteria, bioimprints, ELISA, functionalized silica nanoparticles, horse radish peroxidase 1 INTRODUCTION Bacterial infections remain as a serious issue in modern health care. [1-3] In recent years, this need for the rapid diagnosis of bacterial infections has resulted in the This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Sandwich Elisa-based Bioimprint Detection Methodsmentioning

confidence: 99%

Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

2020

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“…[51,52]; leukemic cells with their higher abundancy than CTCs are addressed in ref. [53]. Given that this is highly challenging, the first applications that can be expected from combining SIPs with HTM readout aim at the detection of bacteria.…”

Section: Detection Of Cancer Cellsmentioning

confidence: 99%

Bioanalytical sensors using the heat-transfer method HTM and related techniques

Wagner,

Bakhshi Sichani,

Khorshid

et al. 2023

Tm - Technisches Messen

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This review provides an overview on bio- and chemosensors based on a thermal transducer platform that monitors the thermal interface resistance R th between a solid chip and the supernatant liquid. The R th parameter responds in a surprisingly strong way to molecular-scale changes at the solid–liquid interface, which can be measured thermometrically, using for instance thermocouples in combination with a controllable heat source. In 2012, the effect was first observed during on-chip denaturation experiments on complementary and mismatched DNA duplexes that differ in their melting temperature. Since then, the concept is addressed as heat-transfer method, in short HTM, and numerous applications of the basic sensing principle were identified. Functionalizing the chip with bioreceptors such as molecularly imprinted polymers makes it possible to detect neurotransmitters, inflammation markers, viruses, and environmental pollutants. In combination with aptamer-type receptors, it is also possible to detect proteins at low concentrations. Changing the receptors to surface-imprinted polymers has opened up new possibilities for quantitative bacterial detection and identification in complex matrices. In receptor-free variants, HTM was successfully used to characterize lipid vesicles and eukaryotic cells (yeast strains, cancer cell lines), the latter showing spontaneous detachment under influence of the temperature gradient inherent to HTM. We will also address modifications to the original HTM technique such as M-HTM, inverted HTM, thermal wave transport analysis TWTA, and the hot-wire principle. The article concludes with an assessment of the possibilities and current limitations of the method, together with a technological forecast.

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Removal of Human Leukemic Cells from Peripheral Blood Mononuclear Cells by Cell Recognition Chromatography with Size Matched Particle Imprints

Cited by 2 publications

References 52 publications

Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

Bioanalytical sensors using the heat-transfer method HTM and related techniques

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