Removal of Escherichia coli from water by systems based on insoluble polystyrene–poly(ethylene glycol)s, –polyethylenimines, and –polyethylenepolyamines quaternized

Abstract

Insoluble polymers adsorbing bacterial cells were prepared by reactions of chloromethylated, divinylbenzene crosslinked polystyrene ( CMPS ) beads with poly (ethylene glycol) 600 (PEGGOO), PEG monolaurate (PEGLE) , polyethylenimines ( PEIs, MW = ca. 300, 600, and 1200, referred to as PEI300, PEIGOO, and PEI1200, respectively), as well as ethylenediamine (ED) and tetraethylenepentamine (TEP) as polyethylenepolyamines. CMPS-ED and CMPS-TEP were further quaternized with 1-iodooctane (10) and 1-iodododecane (IDD) , respectively. These polymers were brought into contact with Escherichia coli by stirring in sterilized, distilled, and deionized water. Although CMPS-PEG and CMPS-PEGLE did not adsorb the cells, they caused a decrease in the number of viable cells. The decrease seemed to result from the bactericidal action of substances leached from the polymers. CMPS-PEI300, CMPS-PEIGOO, and CMPS-PEI1200, CMPS-ED-10, and CMPS-TEP-IDD caused a decrease in the viable cell number by adsorption of the cells to their surfaces. It was observed with a scanning electron microscope that the cells were present on the surfaces of CMPS-PEIGOO beads. The decrease coefficient for decrease in viable cell number of E. coli caused by the polymer increased with nitrogen content of the polymer. The adhesion of the cells to CMPS-PEI300, CMPS-PEIGOO, and CMPS-PEI1200, CMPS-ED-10. and CMPS-TEP-IDD was due mainly to electrostatic interaction between them.

Poly(ethylene imine)s as Antimicrobial Agents with Selective Activity

“…[42] However, Tashiro et al reported bactericidal activity of B-PEI with MW of 600 on E. coli at the concentrations of 25–100 μg/mL in saline. [43, 44] We found that the B-PEI 12 are not active against E. coli (MIC > 1000μg/mL) in MH broth and do not significantly permeabilize the OM of E. coli . However, the lower MW B-PEIs displayed relatively weak inhibitory effects against E. coli (MIC = 250 – 500 μg/mL) although they are also not strong membrane permeabilizers.…”

Antibacterial and Bacterium Adsorbing Macromolecules

“…Insoluble, bacteria adsorbing macromolecules [93] were prepared by grafting of chloromethylated divinylbenzene crosslinked polystyrene beads (CMPS) with poly(ethylene glycol) 600 (PEG600), PEG-mono-dodecyl ether (PEGDD), polyethylenenimines (PEI, MW = ca. 300, 600, and 1 200, referred to as PEI300, PEI600, and PEI1200, respectively), as well as ethylenediamine (ED) and tetraethylenepentamine (TEP).…”

Capture of microbial cells on brush-type polymeric materials bearing different functional groups

“…Only a few studies on the interaction of microbial cells with a brush-type (graftchain-containing) interface have been reported (Kanazawa et al, 1993;Tashiro, 1991). For these brush-type interfaces, the functional groups are not immobilized directly onto the polymeric support but the grafted polymer brushes are.…”

“…Therefore, researchers have been searching for an alternative water disinfectant, such as a solid polymeric material with microbial-cell-capturing activity, to avoid the problem of residual toxicity of soluble chlorine (Kawabata et al, 1983;Tashiro, 1991).…”