Removal of Listeria monocytogenes biofilms on stainless steel surfaces through conventional and alternative cleaning solutions

Mazaheri, T.; Cervantes-Huamán, Brayan R.H.; Turitich, L; Ripolles‐Avila, Carolina; Rodríguez-Jerez, José Juan

doi:10.1016/j.ijfoodmicro.2022.109888

Cited by 10 publications

(4 citation statements)

References 40 publications

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“…It is well known that biofilms have high cell densities ( Flemming et al, 2016 ). On sites with attached bacterial cells and established biofilms, chemical cleaning together with mechanical cleaning strategies such as scraping or enzymatic disruption of biofilms would be essential for biofilm removal ( Chmielewski and Frank, 2003 ; Kumar et al, 2021 ; Mazaheri et al, 2022 ). The top five most abundant genera within the biofilm-positive sample group were Acinetobacter , Pseudomonas , Janthinobacterium , Brevundimonas , and Exiguobacterium .…”

Section: Discussionmentioning

confidence: 99%

Exploring the occurrence of Listeria in biofilms and deciphering the bacterial community in a frozen vegetable producing environment

Pracser,

Voglauer,

Thalguter

et al. 2024

Front. Microbiol.

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The establishment of Listeria (L.) monocytogenes within food processing environments constitutes a significant public health concern. This versatile bacterium demonstrates an exceptional capacity to endure challenging environmental conditions in the food processing environment, where contamination of food products regularly occurs. The diverse repertoire of stress resistance genes, the potential to colonize biofilms, and the support of a co-existing microbiota have been proposed as root causes for the survival of L. monocytogenes in food processing environments. In this study, 71 sites were sampled after cleaning and disinfection in a European frozen vegetable processing facility, where L. monocytogenes in-house clones persisted for years. L. monocytogenes and L. innocua were detected by a culture-dependent method at 14 sampling sites, primarily on conveyor belts and associated parts. The presence of biofilms, as determined by the quantification of bacterial load and the analysis of extracellular matrix components (carbohydrates, proteins, extracellular DNA) was confirmed at nine sites (12.7%). In two cases, L. innocua was detected in a biofilm. Furthermore, we explored the resident microbial community in the processing environment and on biofilm-positive sites, as well as the co-occurrence of bacterial taxa with Listeria by 16S rRNA gene sequencing. Pseudomonas, Acinetobacter, and Exiguobacterium dominated the microbial community of the processing environment. Using differential abundance analysis, amplicon sequence variants (ASVs) assigned to Enterobacterales (Enterobacter, Serratia, unclassified Enterobacteriaceae) and Carnobacterium were found to be significantly higher abundant in Listeria-positive samples. Several Pseudomonas ASVs were less abundant in Listeria-positive compared to Listeria-negative samples. Acinetobacter, Pseudomonas, Janthinobacterium, Brevundimonas, and Exiguobacterium were key players in the microbial community in biofilms, and Exiguobacterium and Janthinobacterium were more relatively abundant in biofilms. Further, the microbial composition varied between the different areas and the surface materials.

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Section: Discussionmentioning

confidence: 99%

Exploring the occurrence of Listeria in biofilms and deciphering the bacterial community in a frozen vegetable producing environment

Pracser,

Voglauer,

Thalguter

et al. 2024

Front. Microbiol.

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“…For the present study, both Gram-positive and Gram-negative food-related bacteria, including foodborne pathogens and spoilage microbes, were selected. Namely, Enterococcus faecalis QSE123 [ 21 ], E. faecalis V583 [ 22 ], Listeria monocytogenes CECT 937 (serotype 3b) [ 23 ], L. monocytogenes CECT 935 (serotype 4b) [ 24 ], Aeromonas hydrophila A259 (from our laboratory collection), and Brochothrix thermosphacta ATCC 11509 T [ 25 ] were selected. To facilitate the visualization of the results, the bacterial strains were given codes, which are used throughout this document (see Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and Antibiofilm Potential of Thymus vulgaris and Cymbopogon flexuosus Essential Oils against Pure and Mixed Cultures of Foodborne Bacteria

et al. 2023

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The spread of pathogenic and food spoilage microorganisms through the food chain still faces major mitigation challenges, despite modern advances. Although multiple cleaning and disinfection procedures are available for microbial load reduction in food-related settings, microbes can still remain on surfaces, equipment, or machinery, especially if they have the ability to form biofilms. The present study assessed the biofilm-forming properties of pure and mixed cultures of foodborne and spoilage bacteria (Listeria monocytogenes, Enterococcus faecalis, Aeromonas hydrophila, Brochothrix thermosphacta), using polystyrene and stainless steel contact surfaces. Subsequently, the antimicrobial and antibiofilm properties of Thymus vulgaris and Cymbopogon flexuosus essential oils—EOs—were evaluated against these bacteria. Moreover, in silico prediction of the absorption and toxicity values of the EOs’ major constituents was also performed, perceiving the putative application in food-related settings. Overall, biofilm formation was observed for all microbes under study, at different temperatures and both contact surfaces. In polystyrene, at 25 °C, when comparing pure with mixed cultures, the combination Listeria–Aeromonas achieved the highest biofilm biomass. Moreover, at 4 °C, increased biofilm formation was detected in stainless steel. Regarding thyme, this EO showed promising antimicrobial features (especially against A. hydrophila, with a MIC of 0.60 µg/µL) and antibiofilm abilities (MBEC of 110.79 µg/µL against L. monocytogenes, a major concern in food settings). As for lemongrass EO, the highest antimicrobial activity, with a MIC of 0.49 µg/µL, was also observed against L. monocytogenes. Overall, despite promising results, the in situ effectiveness of these essential oils, alone or in combination with other antimicrobial compounds, should be further explored.

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“…Two different coupons were used as a growth surface: SS, to simulate general industrial equipment surface, 40 and CI, as it is one of the most frequent materials used for DWDS in North America. 41 All coupons were 1.27 cm in diameter.…”

Section: Methodsmentioning

confidence: 99%

“…The reactor was operated in batch mode for 24 h at low rpm (∼50 rpm). Then, the reactor was set to continuous flow reactor mode with 3.33% of TSB solution and a flow rate of 10 mL/min for another 24 h. Two different coupons were used as a growth surface: SS, to simulate general industrial equipment surface, 40 and CI, as it is one of the most frequent materials used for DWDS in North America. 41 All coupons were 1.27 cm in diameter.…”

Section: Experimental Setup For Biofilm Growth In Cdc Reactorsmentioning

confidence: 99%

Comparison of Legionella pneumophila and Pseudomonas fluorescens Quantification Methods for Assessing UV LED Disinfection

Lara de Larrea,

MacIsaac,

Rauch

et al. 2023

ACS EST Water

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This study assesses the efficacy of ultraviolet light-emitting diodes (UV LEDs) for deactivating Legionella pneumophila (pure culture) and Pseudomonas fluorescens (pure culture and biofilms) on relevant drinking water distribution system surfaces (cast iron and stainless steel). UV LED treatment at 280 nm demonstrated superior performance compared to that at 365 nm, achieving a 4.8 log reduction value (LRV) for P. fluorescens pure cultures and, for biofilms, 4.02 LRV for stainless steel and 2.96 LRV for cast iron at 280 nm. Conversely, the results were less effective at 365 nm, with suspected photolytic reactions on cast iron. Quantification of L. pneumophila yielded varying results: 4 LRV using standard plate counts, 1.8 LRV with Legiolert, and 1 LRV with quantitative polymerase chain reaction at 280 nm, while the results were less than 1.5 LRV at 365 nm. This study provides insights into managing opportunistic pathogens and biofilms, emphasizing the need for improved quantification tools to better assess treatment efficacy.

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Removal of Listeria monocytogenes biofilms on stainless steel surfaces through conventional and alternative cleaning solutions

Cited by 10 publications

References 40 publications

Exploring the occurrence of Listeria in biofilms and deciphering the bacterial community in a frozen vegetable producing environment

Exploring the occurrence of Listeria in biofilms and deciphering the bacterial community in a frozen vegetable producing environment

Antimicrobial and Antibiofilm Potential of Thymus vulgaris and Cymbopogon flexuosus Essential Oils against Pure and Mixed Cultures of Foodborne Bacteria

Comparison of Legionella pneumophila and Pseudomonas fluorescens Quantification Methods for Assessing UV LED Disinfection

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