This study was performed to measure the potential utilization of agro-waste to generate nanoparticles and evaluate the capability of it's as a low cost adsorbent for removal of phenol. Adsorption studies for phenol removal by using Aloe vera and Mesquite leaves nanoparticles carried out under various experimental conditions including pH, nanobioadsorbent dosage, phenol concentration, contact time, temperature and ionic strength in a batch reactor. The adsorption kinetics by pseudo-first order and pseudo-second order models and isotherm technique by Freundlich and Langmuir isotherms models were applied. The results showed that the rate of phenol adsorption increases in both nano-bioadsorbents with an increase of pH up to 7, adsorbent dosage up to 0.08 gL -1 , phenol initial concentration up to 32 mgL -1 , contact time up to 60 min and raising in temperature. The adsorption data followed the Freundlich isotherm model. The kinetic studies indicated that the adsorption of phenol with nano-bioadsorbents was best described by the pseudo second order kinetics. We found that the nanoparticles prepared from Aloe vera and Mesquite leaves had a high capability in adsorption of phenol, beside the point that they could be accessed at a low cost. These agrowastes can be used to remove phenol from aqueous environments.