Removal of Oral Biofilm on an Implant Fixture by a Cavitating Jet

Yamada, Junki; Takiguchi, Tetsuya; Saito, Akihiro; Odanaka, Hibiki; Soyama, Hitoshi; Yamamoto, Masahide

doi:10.1097/id.0000000000000681

Cited by 13 publications

(7 citation statements)

References 20 publications

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“…Comparisons with other studies using only water jetting to decontaminate or clean implant surfaces are difficult, because not only the surface contamination-microbial or artificial-or surface models used, but also the physical properties (e.g. water pressure) of devices used were very different [24,[55][56][57].…”

Section: Discussionmentioning

confidence: 99%

Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study

et al. 2022

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Background Peri-implantitis therapy is a major problem in implantology. Because of challenging rough implant surface and implant geometry, microorganisms can hide and survive in implant microstructures and impede debridement. We developed a new water jet (WJ) device and a new cold atmospheric pressure plasma (CAP) device to overcome these problems and investigated aspects of efficacy in vitro and safety with the aim to create the prerequisites for a clinical pilot study with these medical devices. Methods We compared the efficiency of a single treatment with a WJ or curette and cotton swab (CC) without or with adjunctive use of CAP (WJ + CAP, CC + CAP) to remove biofilm in vitro from rough titanium discs. Treatment efficacy was evaluated by measuring turbidity up to 72 h for bacterial re-growth or spreading of osteoblast-like cells (MG-63) after 5 days with scanning electron microscopy. With respect to application safety, the WJ and CAP instruments were examined according to basic regulations for medical devices. Results After 96 h of incubation all WJ and CC treated disks were turbid but 67% of WJ + CAP and 46% CC + CAP treated specimens were still clear. The increase in turbidity after WJ treatment was delayed by about 20 h compared to CC treatment. In combination with CAP the cell coverage significantly increased to 82% (WJ + CAP) or 72% (CC + CAP), compared to single treatment 11% (WJ) or 10% (CC). Conclusion The newly developed water jet device effectively removes biofilm from rough titanium surfaces in vitro and, in combination with the new CAP device, biologically acceptable surfaces allow osteoblasts to grow. WJ in combination with CAP leads to cleaner surfaces than the usage of curette and cotton swabs with or without subsequent plasma treatment. Our next step will be a clinical pilot study with these new devices to assess the clinical healing process.

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Section: Discussionmentioning

confidence: 99%

Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study

et al. 2022

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“…Some proper detergents, such as a chlorhexidine gluconate scrub, are reported to be helpful in decreasing colony counts and removing biofilms (Schwechter et al, 2011 ). Some new technologies, such as low-intensity intermittent ultrasonication-induced bursting of microbubbles (Agarwal et al, 2014 ) and cavitating jets (Yamada et al, 2017 ), have been combined with these traditional therapies. While these novel methods are promising, their efficacy has yet to be shown in the clinic.…”

Section: Strategies Against Microbial Biofilmsmentioning

confidence: 99%

Promising Therapeutic Strategies Against Microbial Biofilm Challenges

Zhang

Chen

et al. 2020

Front. Cell. Infect. Microbiol.

117

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Biofilms are communities of microorganisms that are attached to a biological or abiotic surface and are surrounded by a self-produced extracellular matrix. Cells within a biofilm have intrinsic characteristics that are different from those of planktonic cells. Biofilm resistance to antimicrobial agents has drawn increasing attention. It is well-known that medical device-and tissue-associated biofilms may be the leading cause for the failure of antibiotic treatments and can cause many chronic infections. The eradication of biofilms is very challenging. Many researchers are working to address biofilm-related infections, and some novel strategies have been developed and identified as being effective and promising. Nevertheless, more preclinical studies and well-designed multicenter clinical trials are critically needed to evaluate the prospects of these strategies. Here, we review information about the mechanisms underlying the drug resistance of biofilms and discuss recent progress in alternative therapies and promising strategies against microbial biofilms. We also summarize the strengths and weaknesses of these strategies in detail.

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“…However, air polishing, a mix of air, water, and powder, is not approved for surgical interventions because water, air, and powder are applied unsterile, and the air may cause emphysema while performing flap procedures [ 34 , 35 ]. Effective removal of microorganisms or debris from teeth or implants was demonstrated several times, for example, with water pressure [ 36 ], high-pressure pulsating water [ 37 ], or cavitating systems [ 38 , 39 ] in vitro and with beneficial effects for peri-implantitis treatment in an animal model [ 40 ]. The interest in using pressured water for biofilm removal in the dental field is increasing [ 36 , 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

In-Vitro Biofilm Removal Efficacy Using Water Jet in Combination with Cold Plasma Technology on Dental Titanium Implants

Matthes

Jablonowski

Miebach

et al. 2023

IJMS

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Peri-implantitis-associated inflammation can lead to bone loss and implant failure. Current decontamination measures are ineffective due to the implants’ complex geometry and rough surfaces providing niches for microbial biofilms. A modified water jet system (WaterJet) was combined with cold plasma technology (CAP) to achieve superior antimicrobial efficacy compared to cotton gauze treatment. Seven-day-old multi-species-contaminated titanium discs and implants were investigated as model systems. The efficacy of decontamination on implants was determined by rolling the implants over agar and determining colony-forming units supported by scanning electron microscopy image quantification of implant surface features. The inflammatory consequences of mono and combination treatments were investigated with peripheral blood mononuclear cell surface marker expression and chemokine and cytokine release profiles on titanium discs. In addition, titanium discs were assayed using fluorescence microscopy. Cotton gauze was inferior to WaterJet treatment according to all types of analysis. In combination with the antimicrobial effect of CAP, decontamination was improved accordingly. Mono and CAP-combined treatment on titanium surfaces alone did not unleash inflammation. Simultaneously, chemokine and cytokine release was dramatically reduced in samples that had benefited from additional antimicrobial effects through CAP. The combined treatment with WaterJet and CAP potently removed biofilm and disinfected rough titanium implant surfaces. At the same time, non-favorable rendering of the surface structure or its pro-inflammatory potential through CAP was not observed.

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Removal of Oral Biofilm on an Implant Fixture by a Cavitating Jet

Abstract: The cavitating jet can effectively clean the biofilm formed on the rough surface of the implant screw, especially on the root sector.

Cited by 13 publications

References 20 publications

Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study

Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study

Promising Therapeutic Strategies Against Microbial Biofilm Challenges

In-Vitro Biofilm Removal Efficacy Using Water Jet in Combination with Cold Plasma Technology on Dental Titanium Implants

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