Removal of phenols from acidic environment by horseradish peroxidase (HRP): Aqueous thermostabilization of HRP by polysaccharide additives

Kalaiarasan, Ellappan; Palvannan, Thayumanavan

doi:10.1016/j.jtice.2013.07.003

Cited by 34 publications

(39 citation statements)

References 37 publications

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“…It will cause severe effects on human being [1]. According to Mohammadi et al [2], the concentration of phenols present in the wastewater from the industries is 6-500 mg/L for refineries, 28-3900 mg/L for coal processing and 2.8-1220 mg/L for petrochemical plants.…”

Section: Introductionmentioning

confidence: 99%

Easy removing of phenol from wastewater using vegetable oil-based organic solvent in emulsion liquid membrane process

Othman

Noah

Shu

et al. 2017

Chinese Journal of Chemical Engineering

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Section: Introductionmentioning

confidence: 99%

Easy removing of phenol from wastewater using vegetable oil-based organic solvent in emulsion liquid membrane process

Othman

Noah

Shu

et al. 2017

Chinese Journal of Chemical Engineering

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“…Phenolic compounds are considered some of the most important toxic organic pollutants in the wastewater streams of a wide variety of industrial processes . Wastewater‐containing phenolic compounds are toxic and carcinogenic to humans due to their high toxicity, poor biodegradability, and long‐term ecological damage .…”

Section: Introductionmentioning

confidence: 99%

Immobilization of horseradish peroxidase on modified PAN‐based membranes for the removal of phenol from buffer solutions

Wang

Liu

Zheng³

et al. 2016

Can J Chem Eng

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Polyacrylonitrile ultrafiltration membranes have been widely used in many separation processes. In this paper, horseradish peroxidase was immobilized onto polyacrylonitrile ultrafiltration membrane by crosslinking with glutaraldehyde. The immobilized enzyme possessed a protein loading of 0.025 mg/cm2membrane and a specific activity of 105 U/mgprotein (105 μmol/min/mgprotein). The initial modified and immobilized membranes were observed using SEM and FTIR. Potential applications of HRP membranes were investigated in the removal of phenol through oxidation with the addition of hydrogen peroxide. The optimum pH of the immobilized enzyme was determined to be 6.0, and the optimum hydrogen peroxide concentration to be 30 mmol/L. Almost 100 % removal of phenol (1 ∼ 10 mg/L) from water was achieved by HRP membranes. For high concentrated solutions, successive cycles were successfully used to improve the degree of phenol oxidation. Furthermore, the immobilized horseradish peroxidase was operationally stable. These results suggest that the HRP membrane has promising applications for the removal of phenol.

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“…For this investigation, a 2 3 fractional factorial CCD for three independent variables consisting of eight factorial points, six axial points, and six replicates at the center points leading to a total number of 20 experiments was used in this study. The number of experimental runs was calculated from the following equation :

N = 2^{k} + 2 k + x_{0}

where N is the number of experiments required, k the number of variables, and x 0 is the number of central points. The range of variables investigated is presented in Tab.…”

Section: Methodsmentioning

confidence: 99%

“…The effects of uncontrolled factors were minimized by randomizing the experimental sequence . The three responses were received at 60°C for 24 h. Second degree polynomials, which include all interaction terms, were used to calculate the predicted response by the following equation:

Y = b_{0} + {true \sum}_{i = 1}^{n} b_{i} x_{i} + {[true \sum_{i = 1}^{n} b_{ii} x_{i}]}^{2} + {true \sum}_{i = 1}^{n - 1} {true \sum}_{j = i + 1}^{n} b_{ij} x_{i} x_{j}

where Y is the predicted response, b 0 the constant coefficient, b i the linear coefficients, b ij the interaction coefficients, b ii the quadratic coefficients, and x i , x j are the coded values of variables of the HRP stabilization . Statistical analysis of the model was carried out in order to evaluate the analysis of variance (ANOVA) .…”

Section: Methodsmentioning

confidence: 99%

Efficiency of Carbohydrate Additives on the Stability of Horseradish Peroxidase (HRP): HRP‐Catalyzed Removal of Phenol and Malachite Green Decolorization from Wastewater

Kalaiarasan

Palvannan

2014

CLEAN Soil Air Water

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The present study was undertaken to develop a cost effective stabilized horseradish peroxidase (HRP) for removal of phenol and decolorization of malachite green from synthetic wastewater. As revealed by enzyme stabilization assays, guar gum and galactose improved the stability of HRP successfully in solution state as compared as other additives such as pectin, carrageenan, or starch, respectively. In order to enhance the stability of HRP, combination of galactose and guar gum were effectively optimized together along with pH by constructing an appropriate response surface methodology (RSM). The RSM results suggest 18.25% of galactose and 0.35% of guar gum for maximum stabilization of HRP at 60°C, pH 7.4. In this study, optimization of removal of phenol and decolorization of malachite green using stabilized HRP was successfully carried out. Various process parameters such as aqueous phase of pH (7.4), concentration of enzyme (1.8 U/mL), phenol concentration (0.1 mM), and reaction time of 180 min were optimized effectively to achieve maximum removal of phenol from synthetic waste water. Dye decolorization studies indicated that stabilized HRP (1.2 U/mL) required lower concentration than the native enzyme (2 U/mL) to obtain similar decolorization efficiency. In addition, stabilized enzyme successfully decolorized the dye at 60°C, pH 7.2.

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Removal of phenols from acidic environment by horseradish peroxidase (HRP): Aqueous thermostabilization of HRP by polysaccharide additives

Cited by 34 publications

References 37 publications

Easy removing of phenol from wastewater using vegetable oil-based organic solvent in emulsion liquid membrane process

Easy removing of phenol from wastewater using vegetable oil-based organic solvent in emulsion liquid membrane process

Immobilization of horseradish peroxidase on modified PAN‐based membranes for the removal of phenol from buffer solutions

Efficiency of Carbohydrate Additives on the Stability of Horseradish Peroxidase (HRP): HRP‐Catalyzed Removal of Phenol and Malachite Green Decolorization from Wastewater

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