Removing bacteria from rough surface titanium discs with chlorhexidine and additional brushing with dentifrice

Park, Jun‐Beom; Koh, Minchul; Jang, Young Eun; Choi, Bong‐Kyu; Kim, Kack-Kyun; Ko, Youngkyung

doi:10.1111/ger.12106

Cited by 6 publications

(5 citation statements)

References 39 publications

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“…The LED toothbrush may have dual action for dental biofilm; brushing is essential for oral hygiene to break the biofilm and LED light may be as effective as photodynamic therapy (PDT). Park et al 31 applied brushing with dentifrice to resorbable blasting media titanium disks incubated with P. gingivalis. The authors described significantly reduced bacterial adherence, but some bacteria remained in pits on the titanium.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study

Park

Kim

et al. 2019

J Dent Rehabil Appl Sci

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The purpose of this study was to evaluate the antimicrobial effects of a toothbrush with light-emitting diodes (LEDs) on periodontitis-associated dental biofilm attached to a zirconia surface by static and dynamic methods. Materials and Methods: Zirconia disks (12 mm diameter, 2.5 mm thickness) were inserted into a 24-well plate (static method) or inside a Center for Disease Control and Prevention (CDC) biofilm reactor (dynamic method) to form dental biofilms using Streptococcus gordonii and Fusobacterium nucleatum. The disks with biofilm were subdivided into five treatment groups-control, commercial photodynamic therapy (PDT), toothbrush alone (B), brush with LED (BL), and brush with LED+erythrosine (BLE). After treatment, the disks were agitated to detach the bacteria, and the resulting solutions were spread directly on selective agar. The number of viable bacteria and percentage of bacterial reduction were determined from colony counts. Scanning electron microscopy (SEM) was performed to visualize alterations in bacterial morphology. Results: No significant difference in biofilm formation was observed between dynamic and static methods. A significant difference was observed in the number of viable bacteria between the control and all experimental groups (P < 0.05). The percentage of bacterial reduction in the BLE group was significantly higher than in the other treated groups (P < 0.05). SEM revealed damaged bacterial cell walls in the PDT, BL, and BLE groups, but intact cell walls in the control and B groups. Conclusion: The findings suggest that an LED toothbrush with erythrosine is more effective than other treatments in reducing the viability of periodontitis-associated bacteria attached to zirconia in vitro.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study

Park

Kim

et al. 2019

J Dent Rehabil Appl Sci

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“…Brushing is essential for oral hygiene management. Park et al [ 24 ] evaluated the effects of brushing on the efficiency of bacterial removal. After resorbable blasting media titanium disks were incubated with P. gingivalis for 2 days, brushing was performed with a dentifrice for a total of 40 seconds, which significantly reduced the amount of bacterial adherence.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of an LED toothbrush on a Porphyromonas gingivalis biofilm on a sandblasted and acid-etched titanium surface: an in vitro study

Lee

Kim

et al. 2018

J Periodontal Implant Sci

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PurposeThe aim of this study was to evaluate the antimicrobial effect of a newly devised toothbrush with light-emitting diodes (LEDs) on Porphyromonas gingivalis attached to sandblasted and acid-etched titanium surfaces.MethodsThe study included a control group, a commercial photodynamic therapy (PDT) group, and 3 test groups (B, BL, and BLE). The disks in the PDT group were placed in methylene blue and then irradiated with a diode laser. The B disks were only brushed, the BL disks were brushed with an LED toothbrush, and the BLE disks were placed into erythrosine and then brushed with an LED toothbrush. After the different treatments, bacteria were detached from the disks and spread on selective agar. The number of viable bacteria and percentage of bacterial reduction were determined from colony counts. Scanning electron microscopy was performed to visualize bacterial alterations.ResultsThe number of viable bacteria in the BLE group was significantly lower than that in the other groups (P<0.05). Scanning electron microscopy showed that bacterial cell walls were intact in the control and B groups, but changed after commercial PDT and LED exposure.ConclusionsThe findings suggest that an LED toothbrush with erythrosine treatment was more effective than a commercial PDT kit in reducing the number of P. gingivalis cells attached to surface-modified titanium in vitro.

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“…To determine the antibacterial effect of LF, cleaning the dental abutment surfaces by mechanical methods, such as brushing and ultrasonic cleaning, and/or chemical rinsing, should be performed before the repeated application of LF solution. 43,44 Further study is needed to confirm the effect of repeated application of LF on dental abutment surfaces with various conditions, such as contamination with oral bacteria other than S. gordonii. It is also necessary to investigate the adsorption behavior of LF in detail, i.e., concentration dependence on the amount of adsorbed LF, time for achieving adsorption equilibrium, and chemical and thermal stability of adsorbed LF, to determine the optimal conditions for LF adsorption on Ti abutment, such as LF concentration of conditioning solution, time required for conditioning, and interval of conditioning.…”

Section: Application Of Lf Adsorption Technique To Inhibit Biofilmmentioning

confidence: 99%

Inhibition of initial bacterial adhesion on titanium surfaces by lactoferrin coating

et al. 2014

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Because dental implant abutments are located at transmucosal sites, their surface should inhibit bacterial accumulation to prevent peri-implantitis. The authors examined the effects of human lactoferrin (LF), an antibacterial protein present in saliva, as an antibacterial coating on the titanium surface and evaluated its effects before and after mucin-containing artificial saliva (AS) incubation. In the control group, titanium disks were soaked in distilled water, whereas in the LF group, titanium disks were soaked in LF solution to coat the disks. In the control-AS and LF-AS groups, half of the control and LF disks were incubated with AS. To confirm LF adsorption, the fluorescence intensity of fluorescein isothiocyanate-labeled LF was measured. The LF and LF-AS groups showed significantly higher intensity than the control and control-AS groups (P < 0.01). There was no significant difference between the LF and LF-AS groups (P > 0.05). The amount of adhered Streptococcus gordonii significantly increased by incubation with AS (P < 0.01) and significantly decreased by adsorption of LF (P < 0.01). There was no interaction between the two factors, LF adsorption and AS incubation (P = 0.561). These results suggest that the adsorbed LF inhibited bacterial adhesion following AS incubation. According to qualitative LIVE/DEAD analysis, viable bacteria appeared to be decreased in the presence of LF and SEM observation indicated that altered morphologies increased in LF and LF-AS groups. These results suggest that the adsorbed LF remained on the titanium surface after incubation with AS, and the remaining LF inhibited bacterial adhesion and exhibited bactericidal effects. Therefore, the adsorption of LF on the abutment material appears to be effective in preventing peri-implantitis.

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Removing bacteria from rough surface titanium discs with chlorhexidine and additional brushing with dentifrice

Abstract: This study clearly showed that burnishing with CHX influenced the RBM titanium surface, and burnishing with CHX pellets and brushing with dentifrice were efficient in removing bacteria from the contaminated titanium surface.

Cited by 6 publications

References 39 publications

Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study

Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study

Efficacy of an LED toothbrush on a Porphyromonas gingivalis biofilm on a sandblasted and acid-etched titanium surface: an in vitro study

Inhibition of initial bacterial adhesion on titanium surfaces by lactoferrin coating

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