Removing endotoxin from metallic biomaterials with compressed carbon dioxide-based mixtures

Tarafa, Pedro J.; Williams, Eve; Panvelker, Samir; Zhang, Jian; Matthews, Michael A.

doi:10.1016/j.supflu.2010.09.010

Cited by 12 publications

(8 citation statements)

References 34 publications

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“…3e shows the effect of adding Ls-54 to the solution to be minimal. Ls-54 is a non-fluorinated surfactant that has been shown in the past to have solubility in supercritical CO 2 and to be an effective scCO 2 additive for removing bacterial endotoxin from a solid surface [47,48]. However, it appears to be ineffective in enhancing decellularizaton, probably because of its dissimilar chemistry compared to SDS.…”

Section: Decellularization With Supercritical Comentioning

confidence: 99%

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

Casali

Handleton

Shazly

et al. 2018

The Journal of Supercritical Fluids

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A B S T R A C TDecellularized tissues are commonly utilized as tissue engineering scaffolds. Decellularization by extended exposure to aqueous detergents can damage the microstructure or deposit cytotoxic residue. Supercritical carbon dioxide (scCO 2 ) has been proposed for decellularization, but reportedly causes dehydration and scaffold embrittlement.Presented herein is a novel decellularization method that preserves matrix hydration state and mechanical properties. Over 97% of the water in porcine aorta is maintained by presaturating scCO 2 with water; however, complete decellularization was not attained by any process utilizing only scCO 2 . Instead, a novel hybrid method is presented that combines a brief (48 h) exposure of tissue to aqueous detergent, followed by washing with scCO 2 (1 h). The hybrid method fully decellularized the tissue, as confirmed by histology and DNA quantification (<0.04 μg DNA/mg tissue). This hybrid treatment was faster than the standard method (2 days compared to 4-7 days), while preserving tissue structure and mechanical properties.

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Section: Decellularization With Supercritical Comentioning

confidence: 99%

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

Casali

Handleton

Shazly

et al. 2018

The Journal of Supercritical Fluids

Self Cite

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“…Modern titanium fixtures have microtextured surfaces created by various combinations of acid‐etching, grit‐blasting, plasma‐spraying, and anodization, to enhance osseointegration (Le Guehennec, Soueidan, Layrolle, & Amouriq, ). These microscopic surface irregularities, when combined with protected areas between implant threads, provide physical protection to the biofilm, making professional cleaning with conventional instruments difficult or impossible (Renvert, Samuelsson, Lindahl, & Persson, ; Tarafa, Williams, Panvelker, Zhang, & Matthews, ).…”

Section: Introductionmentioning

confidence: 99%

“…protection to the biofilm, making professional cleaning with conventional instruments difficult or impossible (Renvert, Samuelsson, Lindahl, & Persson, 2009;Tarafa, Williams, Panvelker, Zhang, & Matthews, 2011).…”

mentioning

confidence: 99%

Effectiveness of implant surface debridement using particle beams at differing air pressures

Wei

Tran

Meredith

et al. 2017

Clinical & Exp Dental Res

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Because implant surface decontamination is challenging, air powder abrasive systems have been suggested as an alternative debridement method. This in vitro study investigated the effectiveness of different powder formulations and air pressures in cleaning implant surfaces and the extent of surface damage. A validated ink model of implant biofilm was used. Sterile 4.1 × 10 mm Grade 4 titanium implants were coated in a blue indelible ink to form a uniform, visually detectable biofilm‐like layer over the implant threads and mounted into a bone replica material with bony defects to approximate peri‐implantitis. Air powder abrasive treatments were undertaken using glycine, sodium bicarbonate, or calcium carbonate powder at air pressures of 25, 35, 45, and 55 psi. Digital macro photographs of the threads were stitched to give composite images of the threads, so the amount of ink remaining could be quantified as the residual area and expressed as a percentage. Implant surfaces were also examined with scanning electron microscopy to grade the surface changes. No treatment cleaned all the surface of the threads. The powders were ranked in order of decreasing effectiveness and decreasing surface change into the same sequence of calcium carbonate followed by sodium bicarbonate followed by glycine. Higher air pressure improved cleaning and increased surface change, with a plateau effect evident. All powders caused some level of surface alteration, with rounding of surface projections most evident. With air powder abrasive systems, there is a trade‐off between cleaning efficacy and surface damage. Using this laboratory model, sodium bicarbonate and calcium carbonate powders were the most effective for surface cleaning when used at air pressures as low as 25 psi.

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“…However, the nuclei were not affected by the treatments (Supplementary Figure S1A,B), and therefore, the tested conditions are ineffective for hASCs and hDFbs cell sheet decellularization. To increase the SCF's density and mass transfer capacity [23], we then considered the use of an entrainer, ethanol, or Dehypon ® . Pressure and time of incubation with the entrainer were also varied with the expectation to further enhance the SCF capacity to transport the DNA molecules from the inside to the outside of the sample.…”

Section: Optimized Supercritical Extractionmentioning

confidence: 99%

“…Additionally, this technology allows for the combination of the SCF with mild detergents, enhancing its efficacy since the polar entrainer/detergent fosters the interaction of the CO 2 with other polar molecules, such as phospholipids and DNA molecules [20,21]. For example, the use of Dehypon ® LS45, a fatty alcohol with surfactant-like properties and the ability to interact, diffuse, and micelle within the scCO 2 due to its alcohol group [22,23], improves decellularization of soft tissues (skin and tendon) [20]. Nonetheless, this was not effective for articular cartilage, and the effect of the polar entrainer/detergent on the ECM content of both soft and hard tissues has yet to be demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Bioinks Enriched with ECM Components Obtained by Supercritical Extraction

et al. 2022

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Extracellular matrix (ECM)-based bioinks have been steadily gaining interest in the field of bioprinting to develop biologically relevant and functional tissue constructs. Herein, we propose the use of supercritical carbon dioxide (scCO2) technology to extract the ECM components of cell-sheets that have shown promising results in creating accurate 3D microenvironments replicating the cell’s own ECM, to be used in the preparation of bioinks. The ECM extraction protocol best fitted for cell sheets was defined by considering efficient DNA removal with a minor effect on the ECM. Cell sheets of human dermal fibroblasts (hDFbs) and adipose stem cells (hASCs) were processed using a customised supercritical system by varying the pressure of the reactor, presence, exposure time, and type of co-solvent. A quantification of the amount of DNA, protein, and sulfated glycosaminoglycans (sGAGs) was carried out to determine the efficiency of the extraction in relation to standard decellularization methodologies. The bioinks containing the extracted ECM were fabricated by combining them with alginate as a support polymer. The influence of the alginate (1%, 2% w/vol) and ECM (0.5% and 1.5% w/vol) amounts on the printability of the blends was addressed by analysing the rheological behaviour of the suspensions. Finally, 3D printed constructs were fabricated using an in-house built extrusion-based bioprinter, and the impact of the extrusion process on cell viability was assessed. The optimised scCO2 protocol allowed efficient removal of DNA while preserving a higher number of proteins and sGAGs than the standard methodologies. The characterization of extract’s composition also revealed that the ECM produced by hDFbs (fECM) and hASCs (aECM) is distinctively affected by the extraction protocols. Furthermore, rheological analysis indicated an increase in viscosity with increasing ECM composition, an effect even more prominent in samples containing aECM. 3D printing of alginate/ECM constructs demonstrated that cell viability was only marginally affected by the extrusion process, and this effect was also dependent on the ECM source. Overall, this work highlights the benefits of supercritical fluid-based methods for ECM extraction and strengthens the relevance of ECM-derived bioinks in the development of printed tissue-like constructs.

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Removing endotoxin from metallic biomaterials with compressed carbon dioxide-based mixtures

Cited by 12 publications

References 34 publications

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

Effectiveness of implant surface debridement using particle beams at differing air pressures

Bioinks Enriched with ECM Components Obtained by Supercritical Extraction

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