Renal Clearance of Essential Amino Acids: Their Competition for Reabsorption by the Renal Tubules

Beyer, Karl H.; Wright, Lemuel D.; Skeggs, Helen R.; Russo, Horace F.; Shaner, Grace A.

doi:10.1152/ajplegacy.1947.151.1.202

Cited by 92 publications

(13 citation statements)

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“…This finding suggests that, in line with observations in other tissues (Beyer et al, 1947;Kamin & Handler, 1951), basic and neutral amino acids move through different carrier systems across the muscle membrane.…”

Section: Movement Of Basic Amino Acidssupporting

confidence: 84%

Differences between resting and insulin-stimulated amino acid transport in frog skeletal muscle

Grinstein¹,

Erlij²

1977

J. Membrain Biol.

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We have compared some features of the resting and the insulin-stimulated uptake of alpha-aminoisobutyrate (AIB) in frog skeletal muscle. We found a substantial difference between the two processes, namely, that resting AIB uptake is Na-independent while the insulin-stimulated fraction of the AIB uptake is Na-dependent. Since the amino acid transport systems in frog skeletal muscle are poorly characterized, we have also surveyed some of their properties. One of the most interesting findings of this survey is that both the uptake and efflux of AIB are inhibited by low concentrations of PCMBS (parachloro-mercury-benzene sulfonic acid 5 X 10(-5) M). In contrast, the carrier mediated transport of basic amino acids is neither inhibited by this mercurial agent nor accelerated by insulin. The action of PCMBS strongly suggests the presence of a critical sulfhydryl group in the amino acid carrier system utilized by AIB. This group is exposed to the outside solution since PCMBS penetrates cell membranes poorly, and in addition its inhibitory actions were reverted by agents that do not penetrate the cell membrane like albumin or glutathione.

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Section: Movement Of Basic Amino Acidssupporting

confidence: 84%

Differences between resting and insulin-stimulated amino acid transport in frog skeletal muscle

Grinstein¹,

Erlij²

1977

J. Membrain Biol.

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“…When the data obtained with glycine, histidine, serine, and threonine are interpreted from the standpoint of the grand mean values, the results suggest that under these experimental conditions some one other or group of other amino acids was successfully competing for or interfering with their reabsorptive mechanisms. The results obtained when glycine was infused alone, as well as the evidence accumulated in a number of other studies (5,11,16,17), all indicate that competition for reabsorption takes place among the individual amino acids.…”

Section: Specific Features Of the Reabsorptive Mechanismsmentioning

confidence: 74%

The Renal Tubular Response to Amino Acid Loading 1

Doolan¹,

Harper²,

Hutchin³

et al. 1956

J. Clin. Invest.

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In previous studies from this laboratory (1) it was shown that renal tubular reabsorption of filtered amino acids was virtually complete in the fasting state. However, when a solution of amino acids was administered intravenously and the load filtered by the kidney thus increased, both the amounts of amino acids reabsorbed and those excreted were increased. In quantitative terms the more noteworthy event was the increase in amount reabsorbed. Considerable selectivity was exhibited in the reabsorption of individual amino acids and evidence was obtained which was interpreted as indicating a "competition" for reabsorption between cystine and one or more other amino acids.In the present studies the solution of amino acids used for loading was administered at a faster rate than in the previously reported experiments and it was supplemented with additional glycine to test the effects of a further increase in the filtered load of amino acids. In separate studies glycine was administered alone and, in terms of amounts reabsorbed and excreted, the results were compared with those obtained when glycine was given as part of the amino acid mixture and comparable filtered loads were achieved. METHODSThree normal subjects-DNW (age 21, ht. 184.2 cm., wt. 85.5 kg.), WRR (age 28, ht. 175.3 cm., wt. 95.0 kg.), and WS (age 29, ht. 168.9 cm., wt. 60.5 kg.)-were given Merck's 10 per cent solution of free amino acids. The experimental protocol is outlined in Table I-A, the rate of infusion being calculated on a basis of its glycine content. The glomerular filtration rate (GFR) was meas-

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“…From the assembled results it is apparent that the change of the fractional reabsorption of amino acids in the residual nephrons prob ably is not associated to any of the described transport systems [1,9,12,17] but that the re- absorption of practically all investigated amino acids is altered.…”

Section: Discussionmentioning

confidence: 95%

Renal Clearance of Amino Acids in Patients with Severe Chronic Renal Failure

Nádvorníková

Schück

Malý

et al. 1978

Nephron

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The renal clearance of 21 amino acids was investigated in 5 patients with chronic renal failure (inulin clearance ≈ 5–10ml/min) during the 6-week administration of a low-protein diet. The results were compared with values obtained in 6 volunteers. The renal clearance of all investigated amino acids (with the exception of Asp, His, Tau) did not differ significantly from healthy controls. The excretion fraction (amino acid clearance /inulin clearance 100) was in all investigated amino acids significantly higher than incontrols with the exception of Tau. The excretion fractions of Asp, Ser, Cys, Tyr, His, Thr, Tau and Gly were, on average, above 20%.The findings suggest that the fractional reabsorption of amino acids in residual nephrons is decreased.

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Renal Clearance of Essential Amino Acids: Their Competition for Reabsorption by the Renal Tubules

Cited by 92 publications

References 0 publications

Differences between resting and insulin-stimulated amino acid transport in frog skeletal muscle

Differences between resting and insulin-stimulated amino acid transport in frog skeletal muscle

The Renal Tubular Response to Amino Acid Loading 1

Renal Clearance of Amino Acids in Patients with Severe Chronic Renal Failure

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