Renal Effects of Gentamicin in Chronic Bile Duct Ligated Rats

Abstract

Patients with advanced cirrhosis and rats with short-term bile duct ligation (BDL) are prone to develop nephrotoxicity from aminoglycosides. In this study, we characterized the renal response to gentamicin in rats with chronic BDL. BDL and sham-operated (SO) rats were given gentamicin (20 and 40 mg/kg/d) for 7 consecutive days, starting on the 18th postoperative day. Administration of gentamicin to SO group caused a decrease in cortical and medullary prostaglandin E(2)(PGE(2)) generation. However, mild reduction in creatinine clearance and an increase in fractional excretion of sodium occurred only in the BDL rats given the high gentamicin dose. This was accompanied by a reduction in cortical and medullary PGE(2) generation and a reduction in plasma nitric oxide production. In conclusion, gentamicin administration to rats with chronic BDL causes impairment of renal function. This happens only after the occurrence of simultaneous multiple insults to the renal protective mechanisms.

The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats

“…The rats in the BDL group underwent the same procedure and the common bile duct was doubly ligated with a 4-0 silk suture and transected between the two ligatures. The rats were subjected to either bile duct ligation (BDL) or the sham operation using aseptic techniques, as previously described by [13].…”

Influence of distal ileum exclusion on hepatic and renal functions in presence of extrahepatic cholestasis

“…Functional changes of the kidney are linked to etiological agents or hemodynamic changes, characteristic of chronic liver disease, in which there is peripheral vasodilation, renal vasoconstriction and activation of the renin-angiotensin system [21][22][23][24] . There is also increased oxidative stress and increased production of free radicals 25,26 , as well as nephrotoxicity caused directly and indirectly by bile acids and bilirubin, especially in situations of renal ischemia 10,[27][28][29][30] . In this study, animals undergoing biliary-enteric anastomosis and ileal exclusion showed elevated levels of urea and creatinine.…”

Differential response related to genotoxicity in multiple organs of cirrhotic rats

“…Accumulating evidence suggests that patients with advanced cirrhosis and rats with short-term bile duct ligation are prone to develop nephrotoxicity from aminoglycosides [27]. In this regard, we were able to evaluate the effect of cirrhosis on kidney cell DNA.…”