Renal medullary AA amyloidosis, hepatocyte dissociation and multinucleated hepatocytes in a 14-year-old free-ranging lioness (Panthera leo)

Abstract

A 14-year-old lioness, originating from Etosha in Namibia, and a member of a pride in Pilanesberg National Park since translocation in 1994, was euthanased due to fight-related vertebral fracture and spinal injury, incurred approximately 6-8 weeks previously. Blood specimens collected at the time of death showed mild anaemia and a leukogram reflecting stress and chronic infection. Necropsy conducted within 2 hours of death was on a dehydrated, emaciated animal with hindquarter wasting and chronic traumatic friction injuries from dragging her hindlegs. There was cellulitis in the region of bite-wounds adjacent to the thoraco-lumbar vertebral fracture, at which site there was spinal cord compression, and there was marked intestinal helminthiasis. The outer renal medullae appeared pale and waxy and the liver was macroscopically unremarkable. Histopathology and electron microscopy of the kidneys revealed multifocal to coalescing deposits of proximal medullary interstitial amyloid, which fluoresced strongly with thioflavine T, and was sensitive to potassium permanganate treatment prior to Congo Red staining, thus indicating inflammatory (AA) origin. There was diffuse hepatocyte dissociation, as well as numerous binucleated and scattered multinucleated (up to 8 nuclei/cell) hepatocytes, with swollen hepatocyte mitochondria, in liver examined light microscopically. Ultrastructurally, the mono-, bi- and multinucleated hepatocytes contained multifocal irregular membrane-bound accumulations of finely-granular, amorphous material both intra-cytoplasmically and intra-nuclearly, as well as evidence of irreversible mitochondrial injury. The incidence and relevance in cats and other species of amyloidosis, particularly with renal medullary distribution, as well as of hepatocyte dissociation and multinucleation, as reported in selected literature, is briefly overviewed and their occurrence in this lioness is discussed.

Apolipoprotein C-III amyloidosis in white lions (Panthera leo)

“…To date, 2 studies have described the histological features of renal amyloidosis in lions. Williams et al 39 reported amyloidosis characterized by proximal medullary interstitial amyloid deposits in a 14-year-old lion from Namibia (originally a wild animal). Newkirk et al 21 described a case of amyloidosis in an 18-year-old captive lion characterized by localized amyloid deposition in the corticomedullary border of the kidney.…”

Bir Ceylanda (Gazella gazella) Generalize Reaktif Amiloidoz Olgusu

“…Dağılımına göre sistemik ve lokal, patogenezine göre primer ve sekonder olarak sınıflandırılmaktadır [2] . Amiloidoz yabani hayvanlardan Sibirya kaplanlarında, vizonlarda (mink), siyah bacaklı kediler ve siyah bacaklı gelinciklerde [2] , çitalarda [3] , Dorcas ceylanında [4] , Arap ceylanında [5] , dağ ceylanında [6] , Rocky dağı büyük boynuzlu koyunlarında [7] , tavşanlarda [8] , aslanlarda [9] , kuğularda ve kınalı kekliklerde [10,11] , deniz aslanı [12] , yunus [13] ve balinalarda [14] bildirilmiştir.…”

Evaluation of goose serum amyloid a acute phase response by enzyme-linked immunosorbent assay

“…Chronically elevated SAA concentrations are a prerequisite for the pathogenesis of secondary amyloidosis, a progressive and fatal disease characterised by the deposition of insoluble unbranched fibrils composed principally of proteolytically cleaved SAA in major organs (Jensen and Whitehead, 1998). AA amyloidosis is a common disease and has been reported in a number of species (Cowan, 1995;Gruber and Linke, 1996;Papendick et al, 1997;Schulze et al, 1998;Ludlage et al, 2005;Williams et al, 2005), including domestic animals (Guo et al, 1996;van der Linde-Sipman et al, 1997;Landman et al, 1998;Mensua et al, 2003;Shtrasburg et al, 2005;Tojo et al, 2005) such as goose (Szabó et al, 2000).…”