Renal Transplantation Normalized Hydrogen Peroxide Production of Neutrophils within the First Day

Abstract

Background: Hemodialysis patients are in a state of oxidant stress. In renal transplantation reactive oxygen species (ROS) are considered to be important factors of ischemia-reperfusion injury. Neutrophils produce ROS as part of the host defense against invading bacteria. This study was designed to investigate whether neutrophil function in hemodialysis patients is immediately affected by renal transplantation. Methods: We evaluated the neutrophil respiratory burst and phagocytic activity in renal transplant patients with living-related donor (LRD) and cadaveric donor (CAD) grafts using flow cytometry techniques. Twenty patients (LRD = 6, CAD = 14) and 20 healthy volunteers were included in the study. Venous blood samples were drawn before anesthesia, 5 min before reperfusion, 1 h and 1, 3 and 7 days after reperfusion. Results: Before surgery, a significant increase in hydrogen peroxide production in neutrophils was seen for both renal transplantation groups compared to healthy subjects. Within 24 h after reperfusion hydrogen peroxide production almost decreased to normal values. The phagocytic capacity of neutrophils was continuously depressed. There were no differences between the CAD and LRD groups. Conclusions: We found that the enhanced respiratory burst activity of patients with chronic renal failure decreased to normal values within 1 day following renal transplantation. Our results suggest that reduced respiratory burst activity resulting in a diminished risk of tissue damage by the uncontrolled production of ROS.

Pleiotropic effects of 4-hydroxynonenal on oxidative burst and phagocytosis in neutrophils

“…In addition, activated neutrophils undergo rapid apoptosis [8] , [44] and NETosis [45] , [46] , releasing DNA, myeloperoxidase and proteases creating a highly reactive and oxidative environment capable of inducing lipid peroxidation and generating reactive lipid species such as 4-HNE. The uncontrolled generation of reactive metabolites in chronic inflammatory diseases has been shown to induce cellular and organelle dysfunction in a variety of cell types [47] , [48] . In vivo and in vitro experimental models support this hypothesis and suggest that increased oxidative stress has profound effects on leukocytes.…”

Reduced post‐operative neutrophil activation in liver transplant recipients suffering from post‐hepatitic cirrhosis

“…Whereas MPO level rose one h after reperfusion, and was significantly increased 24 h after reperfusion in both studied groups. We concluded that this elevation resulting from Kupffer cell activation and generation of ROS, which is characterized by massive neutrophil graft infiltration and further production of the inflammatory mediators (8, 21). In this context we found CRP, which is produced only by hepatocytes (22), and PCT levels significantly increased from 1 to 24 h after reperfusion (Table 2).…”

“…During liver transplantation, the reperfusion phase is associated with Kupffer cell activation, release of pro‐inflammatory cytokines such as TNF‐α, interleukin 1, activated complement factors, platelet‐activating factor, and generation of ROS (8). These cytokines, together with the increased expression of adhesion molecules by sinusoidal endothelial cells, promote liver neutrophil infiltration, thus contributing to the progression of parenchymal injury (17).…”

“…Respiratory burst activity of neutrophils was determined with the content of rhodamine in the phagocytic cells, expressed as mean FL intensity of the FL 1 histogram, as we described recently (8).…”

Unsaturated long-chain fatty acids induce the respiratory burst of human neutrophils and monocytes in whole blood

“…In addition, as we described recently [ 18 ], respiratory burst activity was determined with the content of rhodamine (FL 1) in the phagocytic cells, expressed as mean fluorescence intensity of the FL 1 histogram.…”