Renin in acute myeloid leukaemia blasts

Wulf, Gerald; Jahns-Streubel, G.; Nobiling, R.; Strutz, Frank; Hemmerlein, Bernhard; Hiddemann, Wolfgang; Wörmann, Bernhard

doi:10.1046/j.1365-2141.1998.00565.x

Cited by 32 publications

(25 citation statements)

References 13 publications

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“…For amplification of renin cDNA oligonucleotide primers and conditions were used as previously described [9]. Amplification of kidney RNA served as positive control.…”

Section: Methodsmentioning

confidence: 99%

“…Wulf et al, have demonstrated that myeloid blast cells have renin in the cytosol [8] and recently they have also described that BM blast cells from some acute myeloid leukemia (AML) expressed renin [9]. This finding could be responsible for the activation of the RAS [8,10] and the presence of hypokalemia in 25 -59% of the untreated AML patients [10,11] which could be a mechanism protecting from the hyperkalemia usually associated to AML [12] In accordance with these results, we investigated the expression of renin in BM of patients with hematological malignancies and found a significant renin expression in cells from AML patients.…”

Section: Introductionmentioning

confidence: 97%

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Renin Expression in Hematological Malignancies and its Role in the Regulation of Hematopoiesis

Casares

Iglesia

Perera

et al. 2002

Leukemia & Lymphoma

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It has been demonstrated that some myeloid blasts express renin, but normal bone marrow (BM) does not display this expression. The aim of the present work was to analyze the renin expression in different hematological malignancies and different myeloid cell lines. We investigated the expression of renin by RT-PCR in BM from patients with hematological malignancies (106 patients), in nine normal BM from healthy donors and in leukemic cell lines (K562, KU812, MEG-01, U-937 and HL60), as well in K562 cell line subjected to differentiation treatments. We have observed renin expression in cells from acute myeloid leukemia (AML), chronic myelogenous leukemia (CML) and acute lymphoblastic leukemia (ALL) cases. The highest frequency was observed in AML-non acute promyelocytic leukemia(APL) cases (47.2% of the cases). The disappearance of this expression was associated with the status of complete remission of AML. Renin is expressed in some myeloid human leukemia cell lines such as K562, KU812 and MEG-01. However, when K562 cells were treated with inducers of growth inhibition and/or differentiation, the expression did not disappear, indicating that renin expression is associated with a blastic phenotype rather than with cell proliferation. The obtained findings suggest that the renin expression could have a role on the disease development and could be used as an aberrant marker of leukemia.

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“…For amplification of renin cDNA oligonucleotide primers and conditions were used as previously described [9]. Amplification of kidney RNA served as positive control.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Renin Expression in Hematological Malignancies and its Role in the Regulation of Hematopoiesis

Casares

Iglesia

Perera

et al. 2002

Leukemia & Lymphoma

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“…Renin-like enzyme activity converting ANGTS to Ang-I had been detected in leukemic blast cells. 24,25 Specific immunoreactive renin-like peptide of 47 kDa was isolated from AML blast cells. 24,25 Renin is expressed in some myeloid human leukemia cell lines such as K562, KU812 and MEG-01.…”

Section: Discussionmentioning

confidence: 99%

“…24,25 Specific immunoreactive renin-like peptide of 47 kDa was isolated from AML blast cells. 24,25 Renin is expressed in some myeloid human leukemia cell lines such as K562, KU812 and MEG-01. 9,18,26 Renin system is present in the K562 leukemic cell line in vitro model.…”

Section: Discussionmentioning

confidence: 99%

Renin-Angiotensin System (RAS) Expressions in Myeloid Leukemic Cell Lines.

Uz¹

2013

UHOD

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The aim of this study is to search critical renin-angiotensin system (RAS) elements in myeloid leukemic cell lines. Human acute myeloid leukemia (AML) cell lines, KG-1 and HL-60, were cultured. We searched for the gene expression of the major RAS components in KG-1 and HL-60 cell lines by quantitative real-time polymerase chain reaction analysis (qRT-PCR). Angiotensin-converting enzyme I (ACE I) and ACE II mRNA expressions were detected in KG-1 AML cell line. Relative expression of ACE I was higher than ACE II expression in this myeloid leukemic cells. Likewise, RENIN, angiotensinogen (ANGTS), and ACE I mRNA expressions were detected in HL-60 promyelocytic cell line. Relative RENIN expression was the highest, whereas ACE I expression was the lowest in HL-60 neoplastic myeloid cells. These findings indicate that there is a biologically active local RAS in the hematopoietic system in normal and pathological states.Keywords: Renin-angiotensin system, KG-1 AML cell line, HL-60 promyelocytic cell line, mRNA expressions ÖZET Miyeloid Lösemik Hücre Dizilerinde Renin-Anjiyotensin Sistem (RAS) Ekspresyonlar›Bu çal›flman›n amac› miyeloid lösemik hücre dizilerinde kritik renin-anjiyotensin sistemi (RAS) elemanlar›n›n varl›¤›n› araflt›rmakt›r. ‹n-san akut miyeloid lösemi (AML) hücre dizileri, KG-1 ve HL-60, kültüre edildi. Temel RAS elemanlar›n›n KG-1 ve HL-60 hücre dizilerindeki gen ekspresyon düzeyleri kuantitatif gerçek zamanl› polimeraz zincir reaksiyonu (qRT-PCR) ile araflt›r›ld›. KG-1 AML hücre dizisinde anjiyotensin-dönüfltürücü enzim I (ACE I) ve ACE II mRNA ekspresyonlar› saptand›; ACE I rölatif ekspresyonu, ACE II ekspresyonundan yüksek idi. Benzer flekilde, HL-60 promyelositik hücre dizisinde RENIN, anjiyotensinojen (ANGTS) ve ACE I mRNA ekspresyonlar› saptand›. HL-60 neoplastik miyeloid hücrelerinde rölatif RENIN ekspresyonu en yüksek iken, ACE I ekspresyonu en düflük düzeyde bulundu. Bu bulgular hematopoetik sistemde normal ve patolojik durumlarda biyolojik aktif lokal bir RAS varl›¤›n› göstermek-tedir.Anahtar Kelimeler: Renin-anjiyotensin sistemi, KG-1 AML hücre dizisi, HL-60 promiyelositik hücre dizisi, mRNA ekspresyonlar› ULUSLARARASı HEMATOLOJI-ONKOLOJI DERGISI ARTICLE

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“…It has been demonstrated that the RAS component renin is expressed in the bone marrow microenvironment as well as in hematopoietic cells (Abali et al, 2002). Bone marrow blast cells of some types of AML (M4 and M5 FAB types) express renin (Wulf et al, 1998), however no expression has been detected in normal bone marrow from healthy donors (Gómez Casares et al, 2002). Our group also detected renin expression in AML patients, but it did not relate to monocytic dififerentiation or to the existence of other cytogenetic risk markers (Gómez Casares et al, 2002).…”

Section: Other Molecular Markers In Research: Renin Expressionmentioning

confidence: 99%