Renoprotective and hepatoprotective activity of
            <i>Lepidium draba L.</i>
            extracts on oxymetholone‐induced oxidative stress in rat

Feng, Jinge; Gao, Hongbo; Yang, Lin; Xie, Yu; El-Kenawy, Ayman El-Meghawry; El‐kott, Attalla F.

doi:10.1111/jfbc.14250

Cited by 5 publications

(3 citation statements)

References 51 publications

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“…In this study, dilated congested blood vessels appeared between the tubules in the oxymetholone-treated group. This finding was attributed to oxymetholone toxicity [ 4 , 36 ]. Additionally, basal insertion of sperm in between spermatogenic cells may be due to rapid disruption of the junctions between Sertoli and germ cells [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Platelet-Rich Plasma on the Oxymetholone-Induced Testicular Toxicity

et al. 2023

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Oxymetholone is one of the anabolic steroids that has widely been used among teenagers and athletes to increase their muscle bulk. It has undesirable effects on male health and fertility. In this study, the therapeutic effects of platelet-rich plasma (PRP) on oxymetholone-induced testicular toxicity were investigated in adult albino rats. During the experiments, 49 adult male albino rats were divided into 4 main groups: Group 0 (donor group) included 10 rats for the donation of PRP, Group I (control group) included 15 rats, Group II included 8 rats that received 10 mg/kg of oxymetholone orally, once daily, for 30 days, and Group III included 16 rats and was subdivided into 2 subgroups (IIIa and IIIb) that received oxymetholone the same as group II and then received PRP once and twice, respectively. Testicular tissues of all examined rats were obtained for processing and histological examination and sperm smears were stained and examined for sperm morphology. Oxymetholone-treated rats revealed wide spaces in between the tubules, vacuolated cytoplasm, and dark pyknotic nuclei of most cells, as well as deposition of homogenous acidophilic material between the tubules. Electron microscopic examination showed vacuolated cytoplasm of most cells, swollen mitochondria, and perinuclear dilatation. Concerning subgroup IIIa (PRP once), there was a partial improvement in the form of decreased vacuolations and regeneration of spermatogenic cells, as well as a reasonable improvement in sperm morphology. Regarding subgroup IIIb (PRP twice), histological sections revealed restoration of the normal testicular structure to a great extent, regeneration of the spermatogenic cells, and most sperms had normal morphology. Thus, it is recommended to use PRP to minimize structural changes in the testis of adult albino rats caused by oxymetholone.

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Section: Discussionmentioning

confidence: 99%

“…Some studies demonstrated that oxymetholone was associated with oxidative stress and cellular damage in many organs, particularly the kidney and the liver [ 4 ], as well as inducing disturbances in the testicular enzymes and hormones [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Platelet-Rich Plasma on the Oxymetholone-Induced Testicular Toxicity

et al. 2023

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“…ALOF extract was received, and the procedure was similar to the previous step. Finally, the lowest dose in which toxic symptoms or death were observed ( D L ), along with the highest dose ( D S ) in which rats did not have any toxic symptoms, were placed in the following formula and LD 50 of ALOF extract was calculated (Feng et al, 2022).

normalL D_{50} goodbreak= ()(, D_{L} goodbreak\times D_{S})

…”

Section: Methodsmentioning

confidence: 99%

Protective effects of Althaea officinalis L. extract against N‐diethylnitrosamine‐induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti‐inflammatory, mitochondrial apoptosis and PI3K/Akt/mTOR signaling pathways

Wang

Jiang

Zhang

et al. 2023

Food Science & Nutrition

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Hepatocellular carcinoma is the fourth cause of death due to cancer and includes 90% of liver tumors. Therefore, in this study, it was tried to show that Althaea officinalis L. flower extract (ALOF) can protect hepatocytes against N‐diethylnitrosamine (DEN)‐induced hepatocellular carcinoma. Totally, 70 Wistar rats were divided into seven groups (n = 10/group) of sham, DEN, treatment with silymarin (SIL; DEN + SIL), treatment with ALOF (DEN + 250 and 500 ALOF), and cotreatment with SIL and ALOF (DEN + SIL + 250 and 500 ALOF). At the end of the study, the serum levels of liver indices (albumin, total protein, bilirubin, C‐reactive protein, ALT, AST, and ALP), inflammatory cytokines (IL‐6, IL‐1β, IL‐10, and TNF‐α), and oxidants parameters (glutathione peroxidase [GPx], superoxide dismutase [SOD], catalase [CAT] activity along with nitric oxide [NO] levels) were evaluated. The level of Bax, Bcl‐2, Caspase‐3, p53, PI3K, mTOR, and AKT genes were measured. ALOF in cotreatment with SIL was able to regulate liver biochemical parameters, improve serum antioxidant indices, and decrease the level of proinflammatory cytokines significantly (p < .05). ALOF extract in both doses of 250 and 500 mg/kg in cotreatment with SIL caused a significant (p < .05) decrease in the p53‐positive cells and a significant (p < .05) increase in Bcl‐2‐positive cells. Therefore, ALOF was able to modulate the proliferation of cancer cells and protect normal cells through the regulation of Bax/Bcl‐2/p53 and PI3K/Akt/mTOR signaling pathways. It seems that ALOF can be used as a prodrug or complementary treatment in the protection of hepatocytes in induced damages caused by carcinogens.

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Panicum miliaceum L. and Bone Healing Properties in Rat Model of Femur Fracture by Activating Phosphate Stimulating Macrophages via BMP2 and RANK Signaling Pathway

Zhang,

Wang,

Pan

et al. 2023

Pharmacognosy Magazine

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Objective The aim of this study was to investigate the effect of Panicum miliaceum involved in the repair of femur bone fracture in rats. Materials and Methods In this study, 40 Wistar rats in five groups were studied as healthy, rats with lesion induced by surgery in femur (FX), FX rats treated with P. miliaceum extract (FX +100 and 200 P. miliaceum) and FX rats treated with Osteocare syrup (FX + OC). After 40 days, the serum levels of blood calcium (Ca), phosphorus (P), and alkaline phosphatase (ALP) along with activity of glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) enzymes, and nitric oxide (NO) level were measured. The serum level of cytokines (IL-6, C-reactive protein [CRP], IL-10, IL-1β, and tumor necrosis factor-α [TNF-α]) was also measured by ELISA. The expression of OPG, RANK, RANKL, and BMP-2 genes and proteins were evaluated by real-time PCR and Western blotting techniques, respectively. Results The results showed that P. miliaceum was able to decrease the activity of ALP enzyme in addition to increasing serum Ca and P levels and GPx, SOD, and CAT activity. P. miliaceum reduces serum IL-6, IL-1β, and TNF-α, and NO levels. Also, the extract of this plant could increase the expression of OPG/RANK/BMP-2 pathway genes and proteins. Conclusions P. miliaceum can be used as a prodrug in traditional medicine to repair bone fractures and osteoporosis.

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Renoprotective and hepatoprotective activity of Lepidium draba L. extracts on oxymetholone‐induced oxidative stress in rat

Cited by 5 publications

References 51 publications

Effects of Platelet-Rich Plasma on the Oxymetholone-Induced Testicular Toxicity

Effects of Platelet-Rich Plasma on the Oxymetholone-Induced Testicular Toxicity

Protective effects of Althaea officinalis L. extract against N‐diethylnitrosamine‐induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti‐inflammatory, mitochondrial apoptosis and PI3K/Akt/mTOR signaling pathways

Panicum miliaceum L. and Bone Healing Properties in Rat Model of Femur Fracture by Activating Phosphate Stimulating Macrophages via BMP2 and RANK Signaling Pathway

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