Renoprotective effect of<i>Bacopa monnieri via</i>inhibition of advanced glycation end products and oxidative stress in STZ-nicotinamide-induced diabetic nephropathy

Kishore, Lalit; Kaur, Navpreet; Singh, Randhir

doi:10.1080/0886022x.2016.1227920

Cited by 42 publications

(26 citation statements)

References 59 publications

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“…16 In agreement with GST, animal experiments on LPO have yielded the same results. 17,18 In addition, genetic investigation also found that knockout of GST coding genes can lead to decreased GST levels and increased malondialdehyde (MDA) levels, an important biomarker of LPO, demonstrating that GST has an effect against oxidative stress. 19 Human research was consistent with the experimental studies above.…”

Section: Biomarkers Of Glutathione Antioxidant System and Lipid Peroxmentioning

confidence: 99%

Advances in early biomarkers of diabetic nephropathy

Zhang

Liu

Qin

2018

Rev. Assoc. Med. Bras.

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Diabetic nephropathy is the main cause of chronic kidney disease, and represents the most common and serious complication of diabetes. The exact pathogenesis is complex and not elucidated. Several factors and mechanisms contribute to the development and outcome of diabetic nephropathy. An early diagnosis and intervention may slow down disease progression. A variety of biological markers associated with diabetic nephropathy were found in recent years, which was important for predicting the occurrence and development of the disease. Therefore, this article provides an overview of early biomarkers that are associated with diabetic nephropathy.

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Section: Biomarkers Of Glutathione Antioxidant System and Lipid Peroxmentioning

confidence: 99%

Advances in early biomarkers of diabetic nephropathy

Zhang

Liu

Qin

2018

Rev. Assoc. Med. Bras.

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“…Antiglycation activity of SalA in vitro was tested by the ability to inhibit the fluorescence of AGEs as described in previous study [19] with a minor modification. Briefly, a reaction mixture of 5 mg/ml bovine serum albumin (BSA) , 0.5 M glucose, 1% glyoxal, 0.1 mM PMSF, 100 U/ml penicillin and 100 U/ml streptomycin in 0.1 M phosphate buffered-saline (PBS), pH 7.4 with different concentrations of SalA was incubated in darkness at 37 °C for 4 days.…”

Section: Methodsmentioning

confidence: 99%

Salvianolic Acid A Protects Against Diabetic Nephropathy through Ameliorating Glomerular Endothelial Dysfunction via Inhibiting AGE-RAGE Signaling

Hou

Qiang

Zhao

et al. 2017

Cell Physiol Biochem

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Background/Aims: Glomerular endothelium dysfunction leads to the progression of renal architectonic and functional abnormalities in early-stage diabetic nephropathy (DN). Advanced glycation end products (AGEs) and receptor for AGEs (RAGE) are proved to play important roles in diabetic nephropathy. This study investigated the role of Salvianolic acid A (SalA) on early-stage DN and its possible underlying mechanism. Methods: In vitro AGEs formation and breaking rate were measured to illustrate the effect of SalA on AGEs. Type 2 diabetic nephropathy rats were induced by high-fat diet and low-dose streptozocin (STZ). After eight-week treatment with SalA 1 mg/kg/day, 24h-urine protein, creatinine clearance was tested and renal structural injury was assessed by PAS and PASM staining. Primary glomerular endothelial cell permeability was evaluated after exposed to AGEs. AGEs-induced RhoA/ROCK and subsequently activated disarrange of cytoskeleton were assessed by western blot and immunofluorescence. Results: Biochemical assay and histological examination demonstrated that SalA markedly reduced endothelium loss and glomerular hyperfiltration, suppressed glomerular hypertrophy and mesangial matrix expansion, eventually reduced urinary albumin and ameliorated renal function. Further investigation suggested that SalA exerted its renoprotective effects through inhibiting AGE-RAGE signaling. It not only inhibited formation of AGEs and increased its breaking in vitro, but also reduced AGEs accumulation in vivo and downregulated RAGE expression. SalA restored glomerular endothelial permeability through suppressing AGEs-induced rearrangement of actin cytoskeleton via AGE-RAGE-RhoA/ ROCK pathway. Moreover, SalA attenuated oxidative stress induced by AGEs, subsequently alleviated inflammation and restored the disturbed autophagy in glomerular endothelial cell and diabetic rats via AGE-RAGE-Nox4 axis. Conclusion: Our study indicated that SalA restored glomerular endothelial function and alleviated renal structural deterioration through inhibiting AGE-RAGE, thus effectively ameliorated early-stage diabetic nephropathy. SalA might be a promising therapeutic agent for the treatment of diabetic nephropathy.

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“…In a 10‐year study of type 2 diabetics, 38% developed microalbuminuria as a symptom of nephropathy (Kishore, Kaur, Kajal, & Singh, ). Abnormalities in the lipoprotein metabolism are associated with patients with diabetic nephropathy due to the development of microalbuminuria (Kishore, Kaur, & Singh, ). In STZ‐diabetic rats, significantly increased the albumin levels in urine thus demonstrating that albuminuria was related to deteriorating kidney function.…”

Section: Discussionmentioning

confidence: 99%

Attenuation of STZ-induced diabetic nephropathy byCucurbita pepoL. seed extract characterized by GCMS

Kaur¹,

Kishore²,

Singh³

2017

J Food Biochem

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The present study was aimed to evaluate the potential of petroleum ether and hydro‐alcoholic extract of Cucurbita pepo (CPE and CHE) in STZ‐induced diabetic nephropathy. GC‐MS analysis of CPE revealed the presence of different fatty acids, heterocyclic compounds, and so on. Diabetes was induced by intraperitoneal administration of STZ (65 mg/kg) for the development of diabetic nephropathy. After 30 days of STZ‐administration, 100, 200, and 400 mg/kg of CPE and CHE were administered for 45 days. CPE and CHE significantly increased body weight, lowered blood glucose levels, and ameliorated kidney hypertrophy index in the STZ‐diabetic rats. The extract also decreased the levels of creatinine, blood urea nitrogen, total cholesterol, triglycerides, AGEs and albumin in urine, respectively. The results indicated that via amelioration oxidative stress and formation of AGEs, C. pepo produced significant nephroprotective effect in STZ‐ induced diabetic nephropathy in rats. Practical applications DN is a syndrome mainly characterized as increase in excretion of urine albumin, urea, uric acid, and creatinine; glomerular lesions and reduce glomerular filtration rate (GFR) in diabetics. Fruit and seeds of Cucurbita pepo have significant hypoglycemic and antioxidant activity. C. pepo possesses antioxidant, antihyperglycemic, antihyperlipidemic, and antiglycation activity and thus, exhibits a protective action in STZ‐induced diabetic nephropathy. Moreover, further work is necessary to elucidate in detail the mechanism of action of C. pepo at the cellular and molecular levels.

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Renoprotective effect ofBacopa monnieri viainhibition of advanced glycation end products and oxidative stress in STZ-nicotinamide-induced diabetic nephropathy

Cited by 42 publications

References 59 publications

Advances in early biomarkers of diabetic nephropathy

Advances in early biomarkers of diabetic nephropathy

Salvianolic Acid A Protects Against Diabetic Nephropathy through Ameliorating Glomerular Endothelial Dysfunction via Inhibiting AGE-RAGE Signaling

Attenuation of STZ-induced diabetic nephropathy byCucurbita pepoL. seed extract characterized by GCMS

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