Renotropic stimulation in rat kidney cell culture

Yun, Gao; Areas, Jorge; Yamamoto, Nobuto; Preuss, Harry G.

doi:10.1016/0024-3205(88)90249-4

Cited by 5 publications

(4 citation statements)

References 11 publications

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“…Thus, while rat renal growth factor was extracted from 24-hour uninephrectomized plasma, no activity was detected in partially purified plasma from any of the kidney donors tested at this time after UNX. Previous reports have found a maximal circu lating renotropic activity in rats at 24 h after UNX [5,13,14]. However, other studies have shown a different time course for this activity in unextracted sera of humans and rabbits, so that maximal activity was reached between 3 and 10 days after UNX, declining thereafter in both species [15,16].…”

Section: Discussionmentioning

confidence: 84%

Partial Purification of a Renotropic Activity from Plasma of Uninephrectomized Human Subjects

Garcı́a-Ocaña

Ortega²,

González‐García³

et al. 1993

Nephron

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A renotropic factor was partially purified by sequential gel filtration and anion exchanger chromatography from plasma of human kidney transplantation donors and a renal cancer patient after uninephrectomy. This activity increased the rate of [³H]thymidine incorporation into DNA in rat cortical tubules, but not in rat liver cells, within the range of 100-200 ng/ml protein. The renotropic activity was detected between 7 and 12 days after uninephrectomy, and at least in 1 case decreased thereafter. This activity was undetected in gel-filtrated plasma of patients after a nonurological surgical procedure. The potency of this renotropic activity and its elution by gel filtration are similar to those displayed by a renal growth factor activity isolated from uninephrectomized rat plasma, as recently reported.

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Section: Discussionmentioning

confidence: 84%

Partial Purification of a Renotropic Activity from Plasma of Uninephrectomized Human Subjects

Garcı́a-Ocaña

Ortega²,

González‐García³

et al. 1993

Nephron

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“…Although this phenomenon has not been previously described, the growth of primary rabbit skin fibroblasts has been found to be inhibited by approximately 10 % in the presence of nephrectomized rabbit serum (Yamamoto et al 1983). Moreover, renal fibroblast contamination of PTC cultures has been suggested to result in attenuation of the net growth‐stimulatory effects of post‐nephrectomy serum (Yamamoto et al 1983; Yun et al 1988). In vivo studies by other investigators (Williams, 1961; Lowenstein & Stern, 1963) further indicate that renotropin stimulates growth of proximal tubule epithelial cells, but not of renal fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

“…Although the existence of a kidney‐specific humoral growth factor, which incites and/or regulates compensatory renal growth, has been long established by parabiotic experiments (Van Vroonhoven, Soler‐Montesinos & Malt, 1972; Austin, Goldin & Preuss, 1981; Malt, 1983), serum injections in live animals (Lowenstein & Stern, 1963; Austin et al 1981; Malt, 1983; Pollock, Nobes, Gyory, Heng & Field, 1996) and in vitro assays (Austin et al 1981; Malt, 1983; Yamada, Kanetake, Saito, Kondo & Yamamoto, 1983; Yamamoto, Kanetake & Yamada, 1983; Yun, Areas, Yamamoto & Preuss, 1988; Esbrit, Garcia Ocana, Garcia Canero, Manzano & Jiminez Clavero, 1991; Garcia Ocana & Esbrit, 1994; Nobe, Pollock, Heng & Field, 1995), its precise identity remains elusive. Attempts at characterization have so far suggested that this factor is species specific (Yamamoto et al 1983; Fine, 1986; Yun et al 1988), unaffected by dialysis or heating (Fine, 1986), and possibly synthesized by the liver and activated by the remnant kidney in a time‐dependent fashion following nephrectomy (Dicker, Morris & Shipolini, 1977; Fine, 1986; Garcia Ocana & Esbrit, 1992).…”

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confidence: 99%

Role of insulin‐like growth factor binding proteins in human post‐nephrectomy proximal tubule cells

Johnson

Saunders

Field

et al. 1998

The Journal of Physiology

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In order to determine the role of the insulin‐like growth factor‐I (IGF‐I)/IGF binding protein (IGFBP) axis in the augmentation of tubule growth and function following reductions in nephron mass, primary cultures of human proximal tubule cells (PTCs) were generated from the histologically normal sections of ten surgically removed kidneys. PTC hypertrophy (cellular protein content), DNA synthesis (thymidine incorporation) and apical sodium‐hydrogen exchange (NHE) activity (ethylisopropylamiloride‐sensitive apical 22Na+ uptake) were measured following 24 h incubation in media supplemented with 10 % pre‐ or post‐nephrectomy sera obtained from these patients. The results were compared with the effects of pre‐ and post‐operative control sera collected from seven patients undergoing retroperitoneal operations not involving removal of renal tissue. Day 1 post‐nephrectomy sera promoted a significant 73 % increase in apical NHE activity, which was accompanied by a significant increase in PTC binding of 125I‐IGF‐I (post‐ vs. pre‐nephrectomy, 163 ± 6 vs. 142 ± 4 fmol (mg protein)−1; P < 0.05). Subsequent post‐nephrectomy sera significantly stimulated PTC protein content and thymidine incorporation, peaking at day 7 (127.7 ± 14.0 and 118.4 ± 9.0 % of pre‐nephrectomy values, respectively; P < 0.05). The growth effects were cell specific, as they were not observed with renal cortical fibroblasts. No change was detected in any of these measured variables following exposure to control sera. Serum IGF‐I and IGFBP‐1 levels did not significantly change over time or between groups. IGFBP‐3 levels progressively decreased in both control and nephrectomized sera from pre‐operative values of 3580 ± 305 and 3360 ± 217 ng ml−1, respectively, to 2670 ± 341 and 2600 ± 347 ng ml−1 at 1 week post‐operation. Serum IGFBP‐2 levels increased to a comparable extent in both controls (day 0 vs. day 7, 2940 ± 1024 vs. 7010 ± 2520 ng ml−1; P < 0.01) and nephrectomized patients (day 0 vs. day 7, 3070 ± 656 vs. 9130 ± 2010 ng ml−1; P < 0.01). The results indicate that nephrectomy engenders the elaboration of one or more humoral factor(s), which promotes increased binding of IGF‐I to PTCs and which may in turn specifically stimulate PTC Na+ transport and growth.

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“…As to the mediator of functional adaptation, a humoral factor is likely: A "renotropic" factor is present in rat plasma 20 hours after total kidney removal (39), and the putative factor conveys to normal recipient rats an increased salt and water excretion and increase in single nephron GFR (7). This stimulating factor in rat plasma was present after 24 hours and then waned (40). We speculate that observed early changes in natriuretic peptide plasma concentration in patients would be compatible with such an acute and transient effect in human plasma.…”

Section: Urine Cgmp and Albumin Excretion And Plasma Aldosterone Afte...mentioning

confidence: 99%

Functional adaptation after kidney tissue removal in patients is associated with increased plasma atrial natriuretic peptide concentration

Azawi

Jensen

et al. 2021

Nephrology Dialysis Transplantation

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Background Following nephrectomy, the remaining kidney tissue adapts by an increase in GFR. In rats, hyperfiltration can be transferred by plasma. We examined whether natriuretic peptides (ANP, BNP) increase in plasma proportionally with kidney mass reduction and, if so, whether the increase relates to an increase in GFR. Methods Patients (n = 54) undergoing partial or total unilateral nephrectomy at two Danish centers were followed for one year in an observational study. Glomerular filtration rate was measured before, 3, and 12 months after surgery. Natriuretic propeptides (proANP and proBNP) and aldosterone were measured in plasma before and at 24 hours, five days, 21 days, three months, and 12 months. Cyclic GMP was determined in urine. Results There was no baseline difference in GFR between total- and partial nephrectomy (90.1 mL/min ±14.6 vs. 82.9±18, p = 0.16). Single-kidney GFR increased after 3 and 12 months (12.0 and 11.9 ml/min/1.73m2, +23.3%). There was no change in measured GFR 3 and 12 months after partial nephrectomy. ProANP and proBNP increased 3-fold 24h after surgery and returned to baseline after five days. The magnitude of acute proANP and proBNP increases did not relate to kidney mass removed. ProANP, not proBNP, increased 12 months after nephrectomy. Plasma aldosterone and urine cGMP did not change. Urine albumin/creatine ratio increased transiently after partial nephrectomy. Blood pressure was similar between groups. Conclusion ANP and BNP increase acutely in plasma with no relation to degree of kidney tissue ablation. After 1year, only unilateral nephrectomy patients display increased plasma ANP which could support adaptation.

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Renotropic stimulation in rat kidney cell culture

Cited by 5 publications

References 11 publications

Partial Purification of a Renotropic Activity from Plasma of Uninephrectomized Human Subjects

Partial Purification of a Renotropic Activity from Plasma of Uninephrectomized Human Subjects

Role of insulin‐like growth factor binding proteins in human post‐nephrectomy proximal tubule cells

Functional adaptation after kidney tissue removal in patients is associated with increased plasma atrial natriuretic peptide concentration

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