Reoviruses

Rosen, Léon

doi:10.1007/978-3-7091-8206-2_2

Cited by 3 publications

(3 citation statements)

References 141 publications

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“…A similar observation was reported in the case of GSV in FHM cells (Plumb et al 1979) and turbot reovirus in EPC cells (Lupiani et al 1989). The refractory nature of Vero and BHK-21 cell lines to SKRV infection indicated the host specificity of the virus, which helps to differentiate it from reoviruses of mammalian origin (Rosen et al 1979, Joklik 1983c) and orbiviruses (Gorman et al 1983). Aquareoviruses were also, in general, found unable to grow in cells of mammalian or avian origin (Meyers 1979, Hsu et al 1989, Dopazo et al 1991.…”

Section: Discussionsupporting

confidence: 73%

Characteristics of a new reovirus isolated from epizootic ulcerative syndrome infected snakehead fish

Mr²,

Rh³

et al. 2001

Dis. Aquat. Org.

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Epizootic ulcerative syndrome (EUS) has been infecting a wide range of fishes in the South and Southeast Asia for the last 2 decades. One reovirus-like agent (snakehead reovirus, SKRV), isolated from an EUS-infected snakehead fish and investigated in the present study, is the only reovirus so far isolated from an EUS-infected fish. SKRV was characterised by the presence of a double-stranded RNA genome with icosahedral symmetry and double capsid. The virus had an average size of 71 nm, a buoyant density of 1.36 g ml -1 in CsCl and lacked a lipid-containing envelope. Apart from the above, the presence of a segmented genome and structural proteins falling into 3 specific size classes confirmed that the virus belongs to the family Reoviridae. SKRV differed from aquareoviruses by the lack of a cytopathic effect (CPE) with syncitium formation and in the segmentation pattern of RNA genome. The resistance to pH (3.0 to 9.0) and heat treatment and inability to multiply in mammalian cell lines and haemagglutinate human 'O' red blood cells (RBCs) differentiated SKRV from the rest of the similar genera in the family Reoviridae. Serological comparison indicated the antigenic distinctness of the isolate from selected American and European aquareoviruses. SKRV grew well in SSN-1 and SSN-3 cells at 25 to 30°C but not in the most common Aquareovirus susceptible coldwater fish cell line -CHSE-214.

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Section: Discussionsupporting

confidence: 73%

Characteristics of a new reovirus isolated from epizootic ulcerative syndrome infected snakehead fish

Mr²,

Rh³

et al. 2001

Dis. Aquat. Org.

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“…These viruses are ubiquitous in nature and have an exceptionally broad host range. Not only are they present in humans, but they have been recovered from wild and laboratory mice (types 2 and 3, respectively), dogs (type 1), cattle (types 1, 2, and 3), chickens (five sterotypes), and various primates (16). Also, since they are endemic within the population but have no known etiological relationship to any diseases, they are unlikely to be the target of an eradiction program.…”

mentioning

confidence: 99%

Evaluation of cell lines and immunofluorescence and plaque assay procedures for quantifying reoviruses in sewage

Ridinger¹,

Spendlove²,

Barnett³

et al. 1982

Appl Environ Microbiol

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Twelve continuous cell lines were tested to determine their sensitivity to reovirus types 1, 2, and 3 isolated from sewage. Madin-Darby bovine kidney (MDBK), rhesus monkey kidney (LLC-MK2), and human embryonic intestinal (intestinal 407) cells were most sensitive, respectively. In a similar study, MDBK cells were more sensitive than LLC-MK2 and Buffalo green monkey kidney (BGM) cells to sewage-isolated, protamine-precipitated reoviruses which had not been serotyped and had no previous cell contact. Sewage-isolated, protamineprecipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count and plaque assay procedures. The immunofluorescence assay is more sensitive and more rapid than the plaque assay. Reoviruses in excess of 104/liter of raw sewage were detected by the immunofluorescent cell count assay. Baby hamster kidney (BHK-21) University of Utah African green monkey kidney (BS-C-1

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“…Until recently, reovirus infections in humans had not been associated with any one particular disease (12,13). Consequently, serological studies of the antibody response to reovirus infection in particular patient groups have been limited.…”

mentioning

confidence: 99%

Enzyme-linked immunosorbent assays for measurement of reovirus immunoglobulin G, A, and M levels in serum

1988

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Reoviruses

Cited by 3 publications

References 141 publications

Characteristics of a new reovirus isolated from epizootic ulcerative syndrome infected snakehead fish

Characteristics of a new reovirus isolated from epizootic ulcerative syndrome infected snakehead fish

Evaluation of cell lines and immunofluorescence and plaque assay procedures for quantifying reoviruses in sewage

Enzyme-linked immunosorbent assays for measurement of reovirus immunoglobulin G, A, and M levels in serum

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