Rep-PCR Analyses Reveal Genetic Variation of Ralstonia solanacearum Causing Wilt of Solanaceaous Vegetables in Bangladesh

Hossain, Md. Belal; Masud, Md. Mostafa; Haque, Mohammad Mahbubul; Uddi̇n, Mai̇n; Alam, Md. Zahangir; Islam, Md. Rashidul

doi:10.1007/s00284-022-02932-3

Cited by 5 publications

(14 citation statements)

References 32 publications

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“…It is possible that this outcome is because the majority of the isolates from this study were purified from wilted pepper and eggplant. Prior studies, including our meta-analysis of 8,000 RSSC isolations, have shown that phylotype I isolates are the most common etiological agents of bacterial wilt on eggplant and pepper while all RSSC phylotypes are commonly isolated from tomato plants (Gurjar et al 2015; Sagar et al 2014; Ramesh et al 2014; Kumar et al 2014; Hossain et al 2022; Lowe-Power et al 2022). Globally, phylotypes II and III have both been occasionally isolated from eggplant and pepper (Cellier and Prior 2010; Ravelomanantsoa et al 2016; Lee et al 2020; Deberdt et al 2014; Bihon et al 2020; N’guessan et al 2013; Sedighian et al 2020; Safni et al 2014), while phylotype IV has been isolated from pepper but has not been reported on eggplant (Safni et al 2014).…”

Section: Discussionmentioning

confidence: 85%

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Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Subedi,

Cowell,

Cope-Arguello

et al. 2023

Preprint

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In South Asia, bacterial wilt pathogens in the Ralstonia solanacearum species complex (RSSC) impose major constraints on eggplant, tomato, and pepper production. To improve the efficacy of bacterial wilt management, the goals of this study were to (1) conduct a survey of RSSC pathogens in Bangladesh and Nepal, (2) characterize the genetic diversity of these isolates, and (3) screen 37 tomato, eggplant, and pepper accessions for resistance to six representative isolates from South Asia. We isolated 99 isolates from Bangladesh and 20 isolates from Nepal and determined that all are phylotype I isolates of the Ralstonia pseudosolanacearum species. We sequenced and assembled draft genomes for 25 isolates. Phylogenomic analyses suggest that there is a wide diversity of endemic phylotype I isolates in South Asia, and possible introductions of two clonal phylotype I lineages into Bangladesh and Nepal. We contextualize our newly described isolates based on prior reports of RSSC diversity in South Asia and global reports of RSSC pathogens on eggplant and pepper. Greenhouse trials revealed multiple tomato, eggplant, and pepper accessions that exhibit promising levels of resistance to six phylotype I isolates from South Asia.

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Section: Discussionmentioning

confidence: 85%

“…Additionally, phylotype IV has become established in the hills of Meghalaya, the Indian state east of Bangladesh (Gurjar et al 2015; Sagar et al 2014). Although RSSC are prevalent pathogens in Bangladesh and Nepal (Ahmed et al 2013; Pradhanang et al 2000; Hossain et al 2022), little is known about their genetic diversity.…”

Section: Introductionmentioning

confidence: 99%

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Subedi,

Cowell,

Cope-Arguello

et al. 2023

Preprint

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“…Rep-PCR is a conventional and broadly used technique in microbiology and genetic diversity. Such as plant pathogenic bacteria wilt of Solanaceaous vegetables R. solanacearum (Hossain et al 2022), fungal disease strawberry anthracnose (Karimi et al 2019), and zoonotic disease brucellosis (Amoupour et al 2019). In this study, using rep-PCR technique, the genetic diversity of 60 walnut bacterial-associated bacteria from different regions was investigated through three sets of primers (ERIC, BOX, and REP).…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus pumilus group classification recently (Husni et al 2021). Species-specific REPs have been identified in many animal and plant-associated bacteria already (Hossain et al 2022;Mirzaei et al 2021). For instance, it has been used on human pathogens (Escherichia coli, Salmonella enterica, and Pseudomonas aeruginosa), plant pathogens (Agrobacterium tumefaciens and Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Genetic diversity of walnut blight-associated bacteria from China using rep-PCR

et al. 2022

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Abstract. Zheng C, Zhu J, Yang X, Fu B. 2022. Short Communication: Genetic diversity of walnut blight-associated bacteria from China using rep-PCR. Biodiversitas 23: 5681-5686. Walnut blight is caused by the bacterium Xanthomonas arboricola pv. juglandis (Xaj), is one of the most serious diseases in walnut production around the world. Understanding the genetic diversity of walnut blight pathogen is of great significance to developing a scientific and reasonable prevention and control strategy. The rep-PCR (Repetitive Sequence Polymerase Chain Reaction) technique is a powerful tool for analyzing plant pathogens. In this paper, using three pairs of rep-PCR universal primers (ERIC, BOX, and REP), a genetic diversity analysis was carried out on walnut blight-associated bacteria mainly isolated from different counties of Hubei Province, China. A study of 60 walnut blight-associated bacteria isolated in 2016 found that they had a high genetic similarity among them. There is no clear correlation between the genetic diversity of these isolates and their geographical or tissue origins. Interestingly, these clusters can also distinguish copper-resistant isolates from sensitive ones. Therefore, rep-PCR is a powerful tool to study the population of walnut pathogenic bacteria.

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“…To get a better estimate of the diversity of potential hosts for Ralstonia, we did a search of the literature using search terms like "weeds", "host range", and older names for the pathogen (Pseudomonas solanacearum, Burkholderia solanacearum). [189][190][191][192][193] 2022 [194][195][196][197][198] Converting strain metadata into a structured format for data science:…”

Section: Article Selection Criteria and Search Strategymentioning

confidence: 99%

A Meta-analysis of the known Global Distribution and Host Range of theRalstoniaSpecies Complex

Lowe‐Power

Chipman

2020

Preprint

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The Ralstonia species complex is a genetically diverse group of plant wilt pathogens. Ralstonia strains are classified by a “phylotype-sequevar” phylogenetic system. Since the development of the phylotype-sequevar system, over one hundred papers have described the genetic diversity of Ralstonia strains isolated from agriculturally important crops, ornamental plants, and plants in natural ecosystems. Our goal is to create a database that contains the reported global distribution and host range of Ralstonia sequevars. In this first release, we have catalogued information from 35 manuscripts that report one or more Ralstonia strain isolated from 50 geographic regions. The database is hosted as a GitHub repository (https://github.com/lowepowerlab/Ralstonia_Global_Diversity) that will be updated regularly.

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Rep-PCR Analyses Reveal Genetic Variation of Ralstonia solanacearum Causing Wilt of Solanaceaous Vegetables in Bangladesh

Cited by 5 publications

References 32 publications

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Short Communication: Genetic diversity of walnut blight-associated bacteria from China using rep-PCR

A Meta-analysis of the known Global Distribution and Host Range of theRalstoniaSpecies Complex

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