Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Lo, David; Hyun, Jeong S.; Chung, Michael T.; Montoro, Daniel T.; Zimmermann, Andrew; Grova, Monica; Lee, Min; Wan, Derrick C.; Longaker, Michael T.

doi:10.3791/4221

Cited by 19 publications

(16 citation statements)

References 13 publications

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“…Similar to our study, a short in vitro pretreatment of human ASCs followed by in vivo engraftment without recombinant protein may be just as advantageous without potentially stimulating neo-osteoclastogenesis [30,31]. Consistent with the findings from other groups [16,18,32], we found that the transplantation of PLGA scaffold seeded with osteogenically induced ASCs from NORM rats significantly promoted calvarial defect repair compared to implantation with PLGA alone.…”

Section: Discussionsupporting

confidence: 91%

A comparison of tissue engineering based repair of calvarial defects using adipose stem cells from normal and osteoporotic rats

Pei

McConda

et al. 2015

Bone

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Repairing large bone defects presents a significant challenge, especially in those people who have a limited regenerative capacity such as in osteoporotic (OP) patients. The aim of this study was to compare adipose stem cells (ASCs) from both normal (NORM) and ovariectomized (OVX) rats in osteogenic potential using both in vitro and in vivo models. After successful establishment of a rat OP model, we found that ASCs from OVX rats exhibited a comparable proliferation capacity to those from NORM rats but had significantly higher adipogenic and relatively lower osteogenic potential. Thirty-two weeks post-implantation with poly (lactic-co-glycolic acid) (PLGA) alone or PLGA seeded with osteogenic-induced ASCs for critical-size calvarial defects, the data from Herovici’s collagen staining and micro-computed tomography suggested that the implantation of ASC-PLGA constructs exhibited a higher bone volume density compared to the PLGA alone group, especially in the NORM rat group. Intriguingly, the defects from OVX rats exhibited a higher bone volume density compared to NORM rats, especially for implantation of the PLGA alone group. Our results indicated that ASC based tissue constructs are more beneficial for the repair of calvarial defects in NORM rats while implantation of PLGA scaffold contributed to defect regeneration in OVX rats.

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Section: Discussionsupporting

confidence: 91%

A comparison of tissue engineering based repair of calvarial defects using adipose stem cells from normal and osteoporotic rats

Pei

McConda

et al. 2015

Bone

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“…In addition, subcapsular transplants of these cells form multilineage ossicles containing marrow cavity in vivo (Figure 5B-iii–v, 7D). We previously observed that high concentrations of BMP2 triggers skeletal reprogramming of adipose stromal cells in mice (Chan et al, 2015a; Lo et al, 2012). Therefore, we tested whether subcutaneous injection of human adipose stroma with high levels of recombinant BMP2 in matrigel can give rise to heterotopic ossicles enriched for PDPN+CD146-CD73+CD164+ hSSCs (Figure 5C-i).…”

Section: Resultsmentioning

confidence: 99%

Identification of the Human Skeletal Stem Cell

Chan

Gulati

Sinha

et al. 2018

Cell

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486

482

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Summary Stem cell regulation and hierarchical organization of human skeletal progenitors remain largely unexplored. Here, we report the isolation of a self-renewing and multipotent human skeletal stem cell (hSSC) that generates progenitors of bone, cartilage, and stroma, but not fat. Self-renewing and multipotent hSSCs are present in fetal and adult bones and can also be derived from BMP2-treated human adipose stroma (B-HAS) and induced pluripotent stem cells (iPSCs). Gene expression analysis of individual hSSCs reveals overall similarity between hSSCs obtained from different sources and partially explains skewed differentiation towards cartilage in fetal and iPSC-derived hSSCs. hSSCs undergo local expansion in response to acute skeletal injury. In addition, hSSC-derived stroma can maintain human hematopoietic stem cells (hHSCs) in serum-free culture conditions. Finally, we combine gene expression and epigenetic data of mouse skeletal stem cells (mSSCs) and hSSCs to identify evolutionarily conserved and divergent pathways driving SSC-mediated skeletogenesis.

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“…None of the women had any major medical comorbidities. The stem cells were isolated through a series of digestions using type II collagenase as previously described [26]. All cells were passage 1 or 2.…”

Section: Methodsmentioning

confidence: 99%

Enhancing In Vivo Survival of Adipose-Derived Stromal Cells Through Bcl-2 Overexpression Using a Minicircle Vector

Hyun

Grova

Nejadnik

et al. 2013

Stem Cells Translational Medicine

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Tissue regeneration using progenitor cell-based therapy has the potential to aid in the healing of a diverse range of pathologies, ranging from short-gut syndrome to spinal cord lesions. However, there are numerous hurdles to be overcome prior to the widespread application of these cells in the clinical setting. One of the primary barriers to effective stem cell therapy is the hostile environment that progenitor cells encounter in the clinical injury wound setting. In order to promote cellular survival, stem cell differentiation, and participation in tissue regeneration, relevant cells and delivery scaffolds must be paired with strategies to prevent cell death to ensure that these cells can survive to form de novo tissue. The Bcl-2 protein is a prosurvival member of a family of proteins that regulate the mitochondrial pathway of apoptosis. Using several strategies to overexpress the Bcl-2 protein, we demonstrated a decrease in the mediators of apoptosis in vitro and in vivo. This was shown through the use of two different clinical tissue repair models. Cells overexpressing Bcl-2 not only survived within the wound environment at a statistically significantly higher rate than control cells, but also increased tissue regeneration. Finally, we used a nonintegrating minicircle technology to achieve this in a potentially clinically applicable strategy for stem cell therapy. STEM CELLS TRANSLATIONAL MEDICINE 2013;2:690 -702

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Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Cited by 19 publications

References 13 publications

A comparison of tissue engineering based repair of calvarial defects using adipose stem cells from normal and osteoporotic rats

A comparison of tissue engineering based repair of calvarial defects using adipose stem cells from normal and osteoporotic rats

Identification of the Human Skeletal Stem Cell

Enhancing In Vivo Survival of Adipose-Derived Stromal Cells Through Bcl-2 Overexpression Using a Minicircle Vector

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